Align Acetolactate synthase isozyme 2 large subunit; AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit; EC 2.2.1.6 (characterized)
to candidate H281DRAFT_00356 H281DRAFT_00356 acetolactate synthase, large subunit
Query= SwissProt::P0DP90 (548 letters) >FitnessBrowser__Burk376:H281DRAFT_00356 Length = 621 Score = 429 bits (1102), Expect = e-124 Identities = 246/573 (42%), Positives = 336/573 (58%), Gaps = 29/573 (5%) Query: 1 MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEH----------LLCRHEQGA 50 M+GA ++ L QGV+T+FGY GGAI+P YDA++ H ++ +EQ A Sbjct: 22 MSGADIILRVLAEQGVDTLFGYSGGAILPTYDAVFRFNESHAARPERQIKFVVPANEQAA 81 Query: 51 AMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQ 110 A GYARA+GK GV + TSGPGATN +T +AD DSIPVV I GQV IG+DAFQ Sbjct: 82 GFMAAGYARASGKVGVFMVTSGPGATNAVTPIADCNGDSIPVVLICGQVPRAAIGSDAFQ 141 Query: 111 EVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKDIQLASGDLEP 170 E V + AC K FLV +L + + AF++A +GRPGPV+VD+PKDIQ G + Sbjct: 142 EAPVFNIMSACAKQVFLVTDPAKLEQTLRTAFEIARTGRPGPVVVDVPKDIQNWVGQYQG 201 Query: 171 WFTT-----------VENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREF 219 T V N + Q +L ++++P+LYVGGGV A A LR+F Sbjct: 202 QGTLHFRGYSDRLQLVANGSRLDEQKGAQFFDLLRQSERPLLYVGGGVIAAGATAELRQF 261 Query: 220 LAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTG 279 ++P TL GLGA+ A +P LGMLGMHG AN+AV++CD LIAVGARFDDRV G Sbjct: 262 AERYEIPVVTTLMGLGAIPAQHPLSLGMLGMHGAACANYAVEDCDFLIAVGARFDDRVAG 321 Query: 280 -KLNTFAPHAS-VIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQ----PLNQYDWQQH 333 + + FA A V H+DID AE+NK+++AH + GD L AL + L+ W Sbjct: 322 GRPDMFAAGARHVAHIDIDEAEINKVKRAHWSHIGDARQALRALMEHESVALSSALWIGW 381 Query: 334 CAQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPEN 393 +LR H YD I ++++LS+ +++T VGQHQMW AQ T P + Sbjct: 382 VTELRQRHGMNYDRSNPLIQPQRVIEKLSEITGGRAIISTGVGQHQMWTAQFYQFTEPRS 441 Query: 394 FITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLL 453 F+TS +GTMGFGLPAA+GAQ+ARP+ V+ I GDGS MN EL T +P+K++LL Sbjct: 442 FLTSGSMGTMGFGLPAAIGAQLARPDALVIDIDGDGSIRMNAGELETASTYGVPVKVLLL 501 Query: 454 DNQRLGMVRQWQQLFFQER-YSETTLTDNPDFLMLASAFGIH-GQHITRKDQVEAALDTM 511 +N+ GM+RQWQ+LF++ R + DF+M A A G + +++ L Sbjct: 502 NNRGDGMIRQWQRLFYEGRTFVSDKALHRKDFVMAARADGFEFACRVETPSELDEKLKAF 561 Query: 512 LNSDGPYLLHVSIDELENVWPLVPPGASNSEML 544 + +GP L V ID+ +V+P+V PG S S M+ Sbjct: 562 VAFEGPAFLEVMIDQNADVFPMVGPGQSYSNMI 594 Lambda K H 0.320 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 884 Number of extensions: 50 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 548 Length of database: 621 Length adjustment: 36 Effective length of query: 512 Effective length of database: 585 Effective search space: 299520 Effective search space used: 299520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate H281DRAFT_00356 H281DRAFT_00356 (acetolactate synthase, large subunit)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.31783.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.4e-204 666.6 0.0 1.6e-204 666.4 0.0 1.0 1 lcl|FitnessBrowser__Burk376:H281DRAFT_00356 H281DRAFT_00356 acetolactate syn Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Burk376:H281DRAFT_00356 H281DRAFT_00356 acetolactate synthase, large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 666.4 0.0 1.6e-204 1.6e-204 1 555 [. 22 595 .. 22 597 .. 0.95 Alignments for each domain: == domain 1 score: 666.4 bits; conditional E-value: 1.6e-204 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydaly..........dselehilvrheqaaahaadG 56 ++ga+i+++ l ++gv+t+fGy GGa+lp yda++ + +++++++ eqaa +a G lcl|FitnessBrowser__Burk376:H281DRAFT_00356 22 MSGADIILRVLAEQGVDTLFGYSGGAILPTYDAVFrfneshaarpERQIKFVVPANEQAAGFMAAG 87 79********************************98876656555568****************** PP TIGR00118 57 yarasGkvGvvlatsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvt 122 yarasGkvGv ++tsGPGatn+vt+ia+ ds+P+v++ Gqv++++iGsdafqe+ ++ i+ ++ lcl|FitnessBrowser__Burk376:H281DRAFT_00356 88 YARASGKVGVFMVTSGPGATNAVTPIADCNGDSIPVVLICGQVPRAAIGSDAFQEAPVFNIMSACA 153 ****************************************************************** PP TIGR00118 123 khsflvkkaedlpeilkeafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykpt....vk 184 k flv++++ l ++l+ afeia tGrPGPv+vd+Pkd+++ +++ + +++++gy + + lcl|FitnessBrowser__Burk376:H281DRAFT_00356 154 KQVFLVTDPAKLEQTLRTAFEIARTGRPGPVVVDVPKDIQNWVGQYQGQGTLHFRGYSDRlqlvAN 219 *********************************************************755111145 PP TIGR00118 185 ghklqikk...aleliekakkPvllvGgGviiaeaseelkelaerlkipvtttllGlGafpedhpl 247 g++l +k +l++++++P+l+vGgGvi+a+a++el+++aer +ipv+ttl+GlGa+p++hpl lcl|FitnessBrowser__Burk376:H281DRAFT_00356 220 GSRLDEQKgaqFFDLLRQSERPLLYVGGGVIAAGATAELRQFAERYEIPVVTTLMGLGAIPAQHPL 285 666665551115679*************************************************** PP TIGR00118 248 algmlGmhGtkeanlavseadlliavGarfddrvtg.nlakfapeak.iihididPaeigknvkvd 311 +lgmlGmhG++ an+av+++d liavGarfddrv+g ++fa+ a+ + hidid aei+k+ +++ lcl|FitnessBrowser__Burk376:H281DRAFT_00356 286 SLGMLGMHGAACANYAVEDCDFLIAVGARFDDRVAGgRPDMFAAGARhVAHIDIDEAEINKVKRAH 351 **********************************8626799****98799**************** PP TIGR00118 312 ipivGdakkvleellkklkeeekkekeWlekieewkkeyilkldeeeesikPqkvikelskllkde 377 + +Gda++ l+ l+++ + ++ W+ ++e+++++ +++d+++ i+Pq+vi++ls+++ + lcl|FitnessBrowser__Burk376:H281DRAFT_00356 352 WSHIGDARQALRALMEHESVALSSA-LWIGWVTELRQRHGMNYDRSNPLIQPQRVIEKLSEITGGR 416 ***************9877665554.6*************************************** PP TIGR00118 378 aivttdvGqhqmwaaqfyktkkprkfitsgGlGtmGfGlPaalGakvakpeetvvavtGdgsfqmn 443 ai++t+vGqhqmw+aqfy++++pr+f+tsg +GtmGfGlPaa+Ga++a p++ v++++Gdgs+ mn lcl|FitnessBrowser__Burk376:H281DRAFT_00356 417 AIISTGVGQHQMWTAQFYQFTEPRSFLTSGSMGTMGFGLPAAIGAQLARPDALVIDIDGDGSIRMN 482 ****************************************************************** PP TIGR00118 444 lqelstiveydipvkivilnnellGmvkqWqelfyeerysetklaselpdfvklaeayGvk.giri 508 el+t++ y++pvk+++lnn+ Gm++qWq+lfye+r + a + dfv a+a G + + r+ lcl|FitnessBrowser__Burk376:H281DRAFT_00356 483 AGELETASTYGVPVKVLLLNNRGDGMIRQWQRLFYEGRTFVSDKALHRKDFVMAARADGFEfACRV 548 **************************************9999888889***********87589** PP TIGR00118 509 ekpeeleeklkealeskepvlldvevdkeeevlPmvapGagldelve 555 e+p+el+eklk +++ ++p++l+v +d++++v+Pmv pG++ +++++ lcl|FitnessBrowser__Burk376:H281DRAFT_00356 549 ETPSELDEKLKAFVAFEGPAFLEVMIDQNADVFPMVGPGQSYSNMIT 595 ********************************************985 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (621 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 10.36 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory