Align propionate--CoA ligase (EC 6.2.1.17) (characterized)
to candidate H281DRAFT_05523 H281DRAFT_05523 propionyl-CoA synthetase (EC 6.2.1.17)
Query= metacyc::MONOMER-21334 (629 letters) >FitnessBrowser__Burk376:H281DRAFT_05523 Length = 648 Score = 648 bits (1671), Expect = 0.0 Identities = 318/633 (50%), Positives = 435/633 (68%), Gaps = 20/633 (3%) Query: 8 ESWKSDPEAFWMEAAKSIDWVEAPSRALDDSNAPLYEWFTDAKVNTCWNAVDRHVEAGRG 67 E W++DP AFW AA S+ W L + WF A N +N +DRHVEAGRG Sbjct: 8 EDWRTDPMAFWARAASSVAWSRPWDEVLTHQPGQRFSWFDGAYTNASYNCLDRHVEAGRG 67 Query: 68 EQTAIIYDSPITHTKRQISYVELRNRVAMLAGALRAKGVEKGDRVIIYMPMIPEALEAML 127 EQ AI ++SP+T T + ++Y LR++VA AG L+ +GV GD V+IYMP+IP+A+ AML Sbjct: 68 EQVAIAFESPVTGTAQTLTYASLRDQVAGFAGVLQLQGVRMGDVVVIYMPVIPQAIIAML 127 Query: 128 ACARLGAVHSVVFGGFAANELAVRIDDAQPKAIIAASCGLEPGRVVHYKPLLDGAIDMAK 187 ACAR+GA H VVFGGFAA+ELA R+ D P+ I+ ASCG E R + YK +D A+ + Sbjct: 128 ACARIGATHCVVFGGFAASELAKRLRDTLPRVIVTASCGFEVKRRIPYKETVDEAVLQSA 187 Query: 188 HKPEFCVIFQREQEVAHLEEGRDYNWHGFQYGVEPAECVPVEGNHPAYILYTSGTTGAPK 247 + +++QRE L E RD +W G +PV HP YIL+TSGTTG+PK Sbjct: 188 WQGPV-IVYQRELGRVELAE-RDVDWERAMCGTYGVSPIPVPAGHPLYILHTSGTTGSPK 245 Query: 248 GVLRPTAGHLVALNWTMKNIYNVDPGDVFWAASDVGWVVGHSYICYGPLIHGNTTIVFEG 307 G+ R T G++ AL W+M NIY PGDVFWAASD+GWVVGHSY+ YGPL++G TT++FEG Sbjct: 246 GIQRDTGGYMTALLWSMNNIYRSLPGDVFWAASDIGWVVGHSYMTYGPLLNGCTTVLFEG 305 Query: 308 KPVGTPDAGTFWRVISEHKVKSFFTAPTAIRAVKREDP---------KGEMLAKYDLSHL 358 KPVGTPDA FWRVI++H+V FTAPTA+RA+K+EDP GE+ +YDL L Sbjct: 306 KPVGTPDAAAFWRVIAKHRVNVLFTAPTALRAIKKEDPGGYQLREPVMGELHWRYDLGSL 365 Query: 359 KALYLAGERADPDTIIWAQKALSVPVIDHWWQTETGWTIAGNP----LGIEELPTKLGSP 414 ++++LAGER D TI WA+ L VPV+DHWWQTE GW +A + LG E + G+ Sbjct: 366 RSVFLAGERCDTTTIEWAEDLLHVPVLDHWWQTEAGWPMAASTAKGALGGER---RRGAA 422 Query: 415 AKAMPGYDVQILDEGGHQMKPGELGAIAVKLPLPPGTLPGLWNAEARFRKSYLEHFPGYY 474 +A+PG+DV+ +D+ G ++ G G + LPLPPG + +WNA R+ +SY FPG+Y Sbjct: 423 GRALPGWDVRCVDDQGAEVDAGASGNVVCGLPLPPGAMTTIWNAHERYERSYFGRFPGWY 482 Query: 475 ETGDAGMIDEDGYLYIMARTDDVINVAGHRLSTGGMEEVLAGHEDVAECAVIGVSDDLKG 534 +TGDAG +D DG++++M RTDDVINVAGHRLSTG +EE+++ + VAECAV+G++ LKG Sbjct: 483 DTGDAGYVDADGFVFVMGRTDDVINVAGHRLSTGAIEEIVSRLDAVAECAVVGMAHPLKG 542 Query: 535 QMPLGFLCLNNGCNRDHGDVVK-EVVKLVRDKIGPVAAFKLAVVVDRLPKTRSGKILRGT 593 Q+PL FL L +G ++D D + VK+VR+++G VA+ + A VV+RLPKTRSGKILR + Sbjct: 543 QVPLVFLVLKDG-SKDGADAAETAAVKVVREQLGAVASMQAAFVVERLPKTRSGKILRRS 601 Query: 594 MVSIADGKEYKMPATIDDPAILDEIKVALQSLG 626 ++ I DGK +PATI+DP +L +++ +++ G Sbjct: 602 LLQIVDGKAVAIPATIEDPGVLVDVERIVKARG 634 Lambda K H 0.319 0.137 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1331 Number of extensions: 64 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 629 Length of database: 648 Length adjustment: 38 Effective length of query: 591 Effective length of database: 610 Effective search space: 360510 Effective search space used: 360510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory