GapMind for Amino acid biosynthesis

 

Aligments for a candidate for leuB in Paraburkholderia bryophila 376MFSha3.1

Align D-malate dehydrogenase (decarboxylating) (EC 1.1.1.83) (characterized)
to candidate H281DRAFT_01534 H281DRAFT_01534 tartrate dehydrogenase/decarboxylase / D-malate dehydrogenase

Query= BRENDA::P76251
         (361 letters)



>lcl|FitnessBrowser__Burk376:H281DRAFT_01534 H281DRAFT_01534
           tartrate dehydrogenase/decarboxylase / D-malate
           dehydrogenase
          Length = 364

 Score =  343 bits (881), Expect = 3e-99
 Identities = 178/362 (49%), Positives = 236/362 (65%), Gaps = 9/362 (2%)

Query: 2   MKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERW-GFALSFEQMEWASCEYYSHHGKMMPD 60
           M+T RIA IPGDGIGKEV+P G +VL+A A     FA  FE  +W   +YY  HG MMP 
Sbjct: 1   MQTYRIATIPGDGIGKEVVPAGAQVLEALARTTKSFAFEFENFDWGG-DYYRKHGVMMPA 59

Query: 61  DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA 120
           D  + +   DAI FG+ G PD + DHI+LWG  LK  + FDQY N+RP R+ PG+  PL 
Sbjct: 60  DGLDAIRGKDAILFGSAGDPD-IADHITLWGLRLKICQGFDQYANVRPTRILPGIDAPLK 118

Query: 121 GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS 180
             +P D+++ +VREN+EGEYS +GGRV++G   E     S+ TR GV+RI+R+AF LAQS
Sbjct: 119 RCKPEDLNWVIVRENSEGEYSGVGGRVHQGHPIEAATDVSIMTRAGVERIMRFAFRLAQS 178

Query: 181 RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV 240
           RPRK LT  TKSN    +M  WDE    +++ +P++ WDK+ +D   AR + +P   D +
Sbjct: 179 RPRKLLTVITKSNAQRHAMVMWDEIALQVSKEFPDVTWDKELVDASTARMINRPASLDTI 238

Query: 241 VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT 300
           VA+NL  DILSDL  A  G++GIAP+ N++PER FPS+FEP+HGSA DI GK +ANP+ T
Sbjct: 239 VATNLHADILSDLAAALAGSLGIAPTGNIDPERRFPSMFEPIHGSAFDIMGKGLANPVGT 298

Query: 301 IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA-HGPKTPDMKGNATTPQVADAICKII 359
            W+  M+L+ LG  D     A N ++ A+E V A     T D+ G ATT QV  A+C  I
Sbjct: 299 FWSVVMLLEHLGELD-----AANRVMQAVEAVTADKSLHTGDLGGTATTAQVTAAVCAFI 353

Query: 360 LR 361
            R
Sbjct: 354 ER 355


Lambda     K      H
   0.321    0.138    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 391
Number of extensions: 17
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 361
Length of database: 364
Length adjustment: 29
Effective length of query: 332
Effective length of database: 335
Effective search space:   111220
Effective search space used:   111220
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory