GapMind for Amino acid biosynthesis

 

Protein CCNA_02900 in Caulobacter crescentus NA1000

Annotation: FitnessBrowser__Caulo:CCNA_02900

Length: 437 amino acids

Source: Caulo in FitnessBrowser

Candidate for 3 steps in Amino acid biosynthesis

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arginine biosynthesis argE lo Predicted argE by GapMind curators (no experimental data) 32% 94% 171 N(alpha)-acyl-glutamine aminoacylase; N-AGA; Axillary malodor releasing enzyme; AMRE; EC 3.5.1.133 38% 245.4
L-proline biosynthesis argE lo Predicted argE by GapMind curators (no experimental data) 32% 94% 171 N(alpha)-acyl-glutamine aminoacylase; N-AGA; Axillary malodor releasing enzyme; AMRE; EC 3.5.1.133 38% 245.4
L-lysine biosynthesis dapL lo N-acetyldiaminopimelate deacetylase; EC 3.5.1.47 (uncharacterized) 32% 94% 166.4 N(alpha)-acyl-glutamine aminoacylase; N-AGA; Axillary malodor releasing enzyme; AMRE; EC 3.5.1.133 38% 245.4

Sequence Analysis Tools

View CCNA_02900 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKSLFAASALALLIATAAQAGPLNVPATQKVISAQLDRDYPALEALYKDIHAHPELGFQE
VETAKKLAAQMRALGFTVTEGVGKTGVVAVLKNGEGPKVLIRTELDGLPMQEKSGLAWAS
QATATWNGEKVFVAHACGHDIHMAAWVGAARQLVAMKAKWKGTLVFVAQPSEETVRGARA
MLDDGLWDKIGGKPDYGFALHVGSGPAGEVYYKAGVLTSTSDGLDITFNGRGGHGSMPSA
TIDPVLMAARFTVDVQSVISREKDPSAFGVVTVGSIQAGSAGNIIPDKARVRGTIRTQDN
AVREKILDGVRRTVKAVTDMAGAPPADLKLTPGGKMVVNDAALTDRTAVVFKAAFGARAV
AQDKPGSASEDYSEFVLAGVPSVYFAIGGSDPAELAKAKAEGREPPVNHSPYFAPVAEPT
IRTGVEAMTLAVLNVLK

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory