Aligments for a candidate for aroC in Caulobacter crescentus NA1000

GapMind for Amino acid biosynthesis

Aligments for a candidate for aroC in Caulobacter crescentus NA1000

Align Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EPSP phospholyase; EC 4.2.3.5 (characterized)
to candidate CCNA_03256 CCNA_03256 chorismate synthase

Query= SwissProt::P12008
         (361 letters)



>FitnessBrowser__Caulo:CCNA_03256
          Length = 372

 Score =  415 bits (1067), Expect = e-121
 Identities = 218/363 (60%), Positives = 259/363 (71%), Gaps = 16/363 (4%)

Query: 1   MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHDLDRRRPGTSRYTTQRR 60
           M+ NT G LFRVTT+GESHG ALGC+VDG PPGI LT   +Q  LD+RRPG  ++ TQR+
Sbjct: 1   MSHNTFGHLFRVTTWGESHGPALGCVVDGCPPGIALTAEMIQVFLDKRRPGNGKFVTQRQ 60

Query: 61  EPDQVKILSGVFEGV------TTGTSIGLLIENTDQRSQDYSAIKDVFRPGHADYTYEQK 114
           EPD V+ILSGVFE        TTGT I L+IENTDQRS+DYS I   FRPGHADY Y  K
Sbjct: 61  EPDAVRILSGVFEDARSNGQRTTGTPISLMIENTDQRSKDYSEIAQAFRPGHADYAYFAK 120

Query: 115 YGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQMGDIPLDIK--DWSQ 172
           YG+RDYRGGGRSSARETA RVAAGA+A+  +    G+ +R  L Q+G   +D    DW+Q
Sbjct: 121 YGVRDYRGGGRSSARETAARVAAGAVARLIIP---GVTVRAALVQIGPHKIDRSNWDWAQ 177

Query: 173 VEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGLGEPVFDRLDADIAH 232
            EQNP++ PD   +   +E +  ++K G S GA V V A+GVPAG G P++ +LDA++A 
Sbjct: 178 TEQNPYWSPDAAIVPVWEEHLEKIRKAGSSTGAVVEVEATGVPAGWGAPLYAKLDAELAA 237

Query: 233 ALMSINAVKGVEIGDGFDVVALRGSQNRDEITK--DG---FQSNHAGGILGGISSGQQII 287
           ALMSINA KGVEIGDGFD  AL G  N D +    DG   FQSNHAGGILGGISSGQ I+
Sbjct: 238 ALMSINAAKGVEIGDGFDSAALTGEDNADTMRMGDDGQPVFQSNHAGGILGGISSGQPIV 297

Query: 288 AHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRQ 347
           A +A KPTSSI +P  T+N  GEE+E+ TKGRHDPCVGIR VP+ EAM A VL D  LR 
Sbjct: 298 ARVAFKPTSSILIPRETVNEAGEEIELRTKGRHDPCVGIRGVPVVEAMTACVLADAFLRH 357

Query: 348 RAQ 350
           R Q
Sbjct: 358 RGQ 360


Lambda     K      H
   0.319    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 415
Number of extensions: 13
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 361
Length of database: 372
Length adjustment: 30
Effective length of query: 331
Effective length of database: 342
Effective search space:   113202
Effective search space used:   113202
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate CCNA_03256 CCNA_03256 (chorismate synthase)
to HMM TIGR00033 (aroC: chorismate synthase (EC 4.2.3.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00033.hmm
# target sequence database:        /tmp/gapView.4993.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00033  [M=351]
Accession:   TIGR00033
Description: aroC: chorismate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.2e-130  421.5   0.0   1.3e-130  421.3   0.0    1.0  1  lcl|FitnessBrowser__Caulo:CCNA_03256  CCNA_03256 chorismate synthase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Caulo:CCNA_03256  CCNA_03256 chorismate synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  421.3   0.0  1.3e-130  1.3e-130       1     350 [.      10     360 ..      10     361 .. 0.96

  Alignments for each domain:
  == domain 1  score: 421.3 bits;  conditional E-value: 1.3e-130
                             TIGR00033   1 lrlttfGeSHgkalgaiidGlPaglelteediqkelkrRrpgqsrltrmrkEeDeveilsGvfe.....G.kT 67 
                                           +r+tt+GeSHg+alg+++dG+P+g+ lt+e+iq  l++Rrpg++++ ++r+E D+v+ilsGvfe     G +T
  lcl|FitnessBrowser__Caulo:CCNA_03256  10 FRVTTWGESHGPALGCVVDGCPPGIALTAEMIQVFLDKRRPGNGKFVTQRQEPDAVRILSGVFEdarsnGqRT 82 
                                           89************************************************************9744444446* PP

                             TIGR00033  68 tGaPiallikNkdvrskdyedikelpRPgHadytylkKYgikdregggrsSaReTaarvaaGavakklLketa 140
                                           tG+Pi+l+i+N+d+rskdy++i++ +RPgHady+y+ KYg++d++gggrsSaReTaarvaaGava+  +    
  lcl|FitnessBrowser__Caulo:CCNA_03256  83 TGTPISLMIENTDQRSKDYSEIAQAFRPGHADYAYFAKYGVRDYRGGGRSSARETAARVAAGAVARLIIPG-- 153
                                           *****************************************************************977655.. PP

                             TIGR00033 141 gieivayvvklgeveleeesakeiskerldkspvrcpdaeaekemeeeidkakkdgdsvGgvvevvvsnvpvg 213
                                            + + a +v++g  ++++++ +     +++++p   pda      ee ++k++k+g s G+vvev +++vp+g
  lcl|FitnessBrowser__Caulo:CCNA_03256 154 -VTVRAALVQIGPHKIDRSNWDW---AQTEQNPYWSPDAAIVPVWEEHLEKIRKAGSSTGAVVEVEATGVPAG 222
                                           .99**************998884...68899****************************************** PP

                             TIGR00033 214 lGeplfdkldaelasallsinAvKgveiGdGFeaasvrGseanDelvle.ddkirrktnnsGGieGGitnGed 285
                                           +G pl+ kldaela+al+sinA+KgveiGdGF++a  +G +  D + +  d++  +++n+ GGi+GGi+ G++
  lcl|FitnessBrowser__Caulo:CCNA_03256 223 WGAPLYAKLDAELAAALMSINAAKGVEIGDGFDSAALTGEDNADTMRMGdDGQPVFQSNHAGGILGGISSGQP 295
                                           ***********************************************99677889****************** PP

                             TIGR00033 286 irvriavKpiptikkplktvdletkekakatkgRhDpcvvpravpvvEamvalvladallekras 350
                                           i+ r+a+Kp+++i  p++tv+ +++e   +tkgRhDpcv +r vpvvEam+a vlada+l++r++
  lcl|FitnessBrowser__Caulo:CCNA_03256 296 IVARVAFKPTSSILIPRETVNEAGEEIELRTKGRHDPCVGIRGVPVVEAMTACVLADAFLRHRGQ 360
                                           *************************************************************9976 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (351 nodes)
Target sequences:                          1  (372 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 7.11
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis