Align Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.- (characterized)
to candidate CCNA_02518 CCNA_02518 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Query= SwissProt::Q9RF06 (475 letters) >FitnessBrowser__Caulo:CCNA_02518 Length = 498 Score = 425 bits (1092), Expect = e-123 Identities = 221/483 (45%), Positives = 328/483 (67%), Gaps = 15/483 (3%) Query: 3 FETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAMRA 62 +E V+GLEVH ++ + SK+FS + FGA PN +++D A PG+LPV+N+ V+ A++ Sbjct: 15 WEMVLGLEVHAQVASKSKLFSGAAVGFGAGPNEQVSLVDAAMPGMLPVLNRFCVEQAVKT 74 Query: 63 AMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGENGYIDIEVD-GETKRIGITRL 121 + L +I +S+FDRKNYFYPD P+ YQISQFDQPI G + +E D G T + I RL Sbjct: 75 GLGLRAQINLKSRFDRKNYFYPDLPQGYQISQFDQPIVGEGVVSVERDDGTTFDVRIERL 134 Query: 122 HMEEDAGKSTHKGE--YSLVDLNRQGTPLIEIVSEPDIRSPKEAYAYLEKLRSIIQYTGV 179 H+E+DAGKS H + + VDLNR GT L+EIVS+PD+R+ +EA AY++KLR+I+ Y G Sbjct: 135 HLEQDAGKSLHDQDPNATYVDLNRAGTALMEIVSKPDMRTSEEAAAYVKKLRTILVYLGT 194 Query: 180 SDVKMEEGSLRCDANISL-RPYGQEKF---------GTKAELKNLNSFNYVRKGLEYEEK 229 D ME+G+LR D N+S+ RP EKF GT+ E+KN+NS+ Y+++ +EYE + Sbjct: 195 CDGDMEKGNLRADVNVSVCRPGDYEKFRATGDFKHLGTRCEIKNVNSYRYIQQAIEYEAR 254 Query: 230 RQEEELLSGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKERVR 289 RQ E L GG++ QETR FD + G+T MR KE + DYRYFP+PD++PL +D AW + + Sbjct: 255 RQIEILEDGGKVDQETRLFDPTKGETRSMRSKEEAHDYRYFPDPDLLPLVLDPAWVKSIE 314 Query: 290 QTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVN 349 +T+PELPD +KA+ ++ GL AYDA VL + + +D+FE+ + G D KL +NW+ + Sbjct: 315 ETLPELPDAKKARLQSQYGLSAYDAGVLIIDADRADYFEAAAK-GRDAKLVANWVTNELL 373 Query: 350 EYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNGL 409 L+ E ++ L ++A +++LIE+GT+SSKIAK+VF + A G +I+E GL Sbjct: 374 AKLSAAGTEFTNSPLPHTDIAQLVELIENGTISSKIAKEVFEHMWAGEGRPAEIVEKRGL 433 Query: 410 VQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLK 469 VQI+D + K +++ + N E K+ K +A+G+ VGQ+MKA+ G+ANP VN+LL+ Sbjct: 434 VQINDTGAIEKAIDDLIAGNPDKAEAVKD-KPQALGWFVGQVMKATGGKANPGTVNELLR 492 Query: 470 QEL 472 ++L Sbjct: 493 KKL 495 Lambda K H 0.313 0.133 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 646 Number of extensions: 32 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 475 Length of database: 498 Length adjustment: 34 Effective length of query: 441 Effective length of database: 464 Effective search space: 204624 Effective search space used: 204624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 52 (24.6 bits)
Align candidate CCNA_02518 CCNA_02518 (aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00133.hmm # target sequence database: /tmp/gapView.7868.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00133 [M=481] Accession: TIGR00133 Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.8e-162 525.7 0.0 5.6e-162 525.5 0.0 1.0 1 lcl|FitnessBrowser__Caulo:CCNA_02518 CCNA_02518 aspartyl/glutamyl-tRN Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Caulo:CCNA_02518 CCNA_02518 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 525.5 0.0 5.6e-162 5.6e-162 3 480 .. 14 495 .. 12 496 .. 0.95 Alignments for each domain: == domain 1 score: 525.5 bits; conditional E-value: 5.6e-162 TIGR00133 3 eyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalnskivse 75 ++e+v+GlEvH q+ ksKlF+ + + pN++v+ v+ ++PG lPvlN+ v++A+k +l l ++i+ lcl|FitnessBrowser__Caulo:CCNA_02518 14 DWEMVLGLEVHAQVASKSKLFSGAAVGFGA-GPNEQVSLVDAAMPGMLPVLNRFCVEQAVKTGLGLRAQIN-L 84 679*************************99.9*************************************77.5 PP TIGR00133 76 vsvFdRKhYfYpDlPkgyqitqqdlPiaedGkleieleeke.keigierlhlEeDtgksqykesdkdkqslvD 147 +s FdRK+YfYpDlP+gyqi+q+d+Pi+ +G++++e +++ ++ierlhlE+D+gks + ++ ++ ++vD lcl|FitnessBrowser__Caulo:CCNA_02518 85 KSRFDRKNYFYPDLPQGYQISQFDQPIVGEGVVSVERDDGTtFDVRIERLHLEQDAGKSLHDQD--PNATYVD 155 9***********************************99877479*****************966..79***** PP TIGR00133 148 fNRsgvPLlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsirlkGq..........e 210 +NR+g+ L+EiV+kPd+++++ea+a++kklr+il yl+ dgd+e+G++R+DvNvs+ G+ + lcl|FitnessBrowser__Caulo:CCNA_02518 156 LNRAGTALMEIVSKPDMRTSEEAAAYVKKLRTILVYLGTCDGDMEKGNLRADVNVSVCRPGDyekfratgdfK 228 *********************************************************7654422222222246 PP TIGR00133 211 kegtrvEiKNlnslksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDYRYfpePdlp 283 + gtr EiKN+ns + i++aieyE Rq+++l++g +v qetr fd +k t s+R+Kee++DYRYfp+Pdl lcl|FitnessBrowser__Caulo:CCNA_02518 229 HLGTRCEIKNVNSYRYIQQAIEYEARRQIEILEDGGKVDQETRLFDPTKGETRSMRSKEEAHDYRYFPDPDLL 301 789********************************************************************** PP TIGR00133 284 pieideevvkekveeklpelPeakrirlkkeyglseedakvlvsdlelldafeevvklikepklavnWileel 356 p+++d+ +vk+ +ee+lpelP+ak++rl+++ygls++da vl+ d++ +d+fe ++k + kl++nW+++el lcl|FitnessBrowser__Caulo:CCNA_02518 302 PLVLDPAWVKS-IEETLPELPDAKKARLQSQYGLSAYDAGVLIIDADRADYFEAAAKGRDA-KLVANWVTNEL 372 **********9.****************************************999887666.*********** PP TIGR00133 357 lgeLnkkkislaeallkpeelaeliklikegkisqksakelleellenkkdpkklieklgliqisdekelvki 429 l++L + ++++++ l +++a+l++li++g+is+k+ake++e++ ++ p +++ek+gl+qi+d+ ++ k+ lcl|FitnessBrowser__Caulo:CCNA_02518 373 LAKLSAAGTEFTNSPLPHTDIAQLVELIENGTISSKIAKEVFEHMWAGEGRPAEIVEKRGLVQINDTGAIEKA 445 ************************************************************************* PP TIGR00133 430 veevikenpkevekyksgkekalkflvGqvmkktkgradpkevekllkell 480 +++ i+ np++ e k+ k +al+++vGqvmk t g+a+p +v++ll+++l lcl|FitnessBrowser__Caulo:CCNA_02518 446 IDDLIAGNPDKAEAVKD-KPQALGWFVGQVMKATGGKANPGTVNELLRKKL 495 ***********998876.6789*************************9988 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (481 nodes) Target sequences: 1 (498 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 7.66 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory