Alignments for a candidate for hisF in Caulobacter crescentus NA1000

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisF in Caulobacter crescentus NA1000

Align imidazole glycerol-phosphate synthase (subunit 2/2) (EC 4.3.2.10) (characterized)
to candidate CCNA_03853 CCNA_03853 imidazole glycerol phosphate synthase, cyclase subunit

Query= BRENDA::Q5NMD6
         (255 letters)



>FitnessBrowser__Caulo:CCNA_03853
          Length = 260

 Score =  327 bits (839), Expect = 1e-94
 Identities = 164/251 (65%), Positives = 200/251 (79%), Gaps = 2/251 (0%)

Query: 3   LCTRIIPCLDVADGRVVKGVNFTDLMDAGDPVEQAKVYDAAGADELCFLDISASHEGRGT 62
           L TRIIPCLDV DGRVVKGVNF  L DAGDPVEQA+ YDAAGADEL FLDI+AS EGRG 
Sbjct: 2   LKTRIIPCLDVKDGRVVKGVNFVSLRDAGDPVEQARAYDAAGADELMFLDITASSEGRGL 61

Query: 63  MLDVVARTAEVCFMPLTVGGGVRQVEDARALLLAGADKVAVNSAAVARPELVAEIADRFG 122
           +LDV++RTAEVCFMP++VGGGVRQV D R LLLAGADKV+VN+AAV  P+L+A  AD FG
Sbjct: 62  ILDVISRTAEVCFMPVSVGGGVRQVSDMRRLLLAGADKVSVNTAAVENPDLIAGGADAFG 121

Query: 123 AQCVVAAID--ARRNGDHWEVYTHGGRRPTGINALDHALNLTRLGAGEILLTSMDKDGTR 180
           +QCVV AID  AR +G  W V+T+GGR+ TGI+ ++ A  +   GAGEILLTSMD+DG +
Sbjct: 122 SQCVVVAIDAKAREDGSGWNVWTYGGRKDTGIDVVEWAAKVVERGAGEILLTSMDRDGAK 181

Query: 181 DGYDLELTRLVADSVPVPVIASGGVGNLDHMVEGVTKGHASALLAASIFHFGQYSLAEAH 240
            GYD+ L + V  +V VPVIASGG G  +H++E   +GHA+A+LAASIFHFG+ S+ EA 
Sbjct: 182 IGYDIPLLQAVTGAVNVPVIASGGAGKTEHLIEAAREGHAAAVLAASIFHFGEISIGEAK 241

Query: 241 EALAKAGLTVR 251
           +A+A AG+ VR
Sbjct: 242 QAMADAGIPVR 252


Lambda     K      H
   0.320    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 278
Number of extensions: 11
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 255
Length of database: 260
Length adjustment: 24
Effective length of query: 231
Effective length of database: 236
Effective search space:    54516
Effective search space used:    54516
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate CCNA_03853 CCNA_03853 (imidazole glycerol phosphate synthase, cyclase subunit)
to HMM TIGR00735 (hisF: imidazoleglycerol phosphate synthase, cyclase subunit)

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00735.hmm
# target sequence database:        /tmp/gapView.17691.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00735  [M=254]
Accession:   TIGR00735
Description: hisF: imidazoleglycerol phosphate synthase, cyclase subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.7e-112  360.8   2.9   1.9e-112  360.6   2.9    1.0  1  lcl|FitnessBrowser__Caulo:CCNA_03853  CCNA_03853 imidazole glycerol ph


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Caulo:CCNA_03853  CCNA_03853 imidazole glycerol phosphate synthase, cyclase subunit
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  360.6   2.9  1.9e-112  1.9e-112       1     254 []       1     252 [.       1     252 [. 0.99

  Alignments for each domain:
  == domain 1  score: 360.6 bits;  conditional E-value: 1.9e-112
                             TIGR00735   1 mlakriipCLdvkdgrvvkGvqfknlrdaGdpvelakkydeeGadelvflditassekretmlevvervaekv 73 
                                           ml  riipCLdvkdgrvvkGv+f +lrdaGdpve a++yd+ Gadel+flditasse+r  +l+v++r+ae  
  lcl|FitnessBrowser__Caulo:CCNA_03853   1 MLKTRIIPCLDVKDGRVVKGVNFVSLRDAGDPVEQARAYDAAGADELMFLDITASSEGRGLILDVISRTAEVC 73 
                                           7999********************************************************************* PP

                             TIGR00735  74 fiPltvgGGiksiedvkkllraGadkvsintaavkapelikeladrfGsqaivvaidakreaeneeakyevti 146
                                           f+P++vgGG+++++d+++ll aGadkvs+ntaav++p+li+  ad fGsq++vvaidak +++   + ++v +
  lcl|FitnessBrowser__Caulo:CCNA_03853  74 FMPVSVGGGVRQVSDMRRLLLAGADKVSVNTAAVENPDLIAGGADAFGSQCVVVAIDAKAREDG--SGWNVWT 144
                                           ************************************************************9987..79***** PP

                             TIGR00735 147 kgGrestdldvvewakeveelGaGeilltsmdkdGtksGydlellkkvkeavkiPviasgGaGkaehleeafl 219
                                           +gGr+ t++dvvewa++v e+GaGeilltsmd+dG k Gyd+ ll++v  av++PviasgGaGk+ehl ea  
  lcl|FitnessBrowser__Caulo:CCNA_03853 145 YGGRKDTGIDVVEWAAKVVERGAGEILLTSMDRDGAKIGYDIPLLQAVTGAVNVPVIASGGAGKTEHLIEAAR 217
                                           ************************************************************************* PP

                             TIGR00735 220 kgkadaaLaasvfhkreltieevkeylaergvkvr 254
                                           +g+a a+Laas+fh++e++i+e k+ +a+ g++vr
  lcl|FitnessBrowser__Caulo:CCNA_03853 218 EGHAAAVLAASIFHFGEISIGEAKQAMADAGIPVR 252
                                           *********************************98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (254 nodes)
Target sequences:                          1  (260 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 7.91
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis