Alignments for a candidate for dapL in Caulobacter crescentus NA1000

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapL in Caulobacter crescentus NA1000

Align N-acetyldiaminopimelate deacetylase; EC 3.5.1.47 (uncharacterized)
to candidate CCNA_02540 CCNA_02540 N-acyl-L-amino acid amidohydrolase

Query= curated2:B7GIC0
         (378 letters)



>FitnessBrowser__Caulo:CCNA_02540
          Length = 432

 Score =  174 bits (442), Expect = 3e-48
 Identities = 120/347 (34%), Positives = 176/347 (50%), Gaps = 19/347 (5%)

Query: 6   RRDLHQIPELGFQEFKTQQYILDYLATLPSERLQIKTWRTGILVRVHGTAPTKTIGYRAD 65
           RRD+H+ PELG QE +T   I   L  L  E ++    +TG++  + G  P K +  RAD
Sbjct: 44  RRDIHENPELGNQEVRTAALIAKELKALGIE-VREGVGKTGVVGVLKGGKPGKVVALRAD 102

Query: 66  MDGLPIDEQTDVPFRS----THEGR----MHACGHDMHMAIALGVLTHV--VHHPIRDDM 115
           MD LP++E+T +PF S    T EGR    MHACGHD H+A+ LG  T +  +   I+  +
Sbjct: 103 MDALPVEEKTGLPFASKVKATWEGRTVPVMHACGHDTHVAMLLGAATVLAGMKQDIQGTV 162

Query: 116 LFIFQPAEEGP-----GGALPMLESDEMKQWMPDMILALHIAPAYPVGTIATKEGLLFAN 170
           +FIFQPAEEGP     GGA  M+    +     D +  +HI P  P       EG  +A+
Sbjct: 163 VFIFQPAEEGPPAGEEGGAKLMIRDGALDNPKVDAVFGVHIGPGDPHELNYRPEGF-YAS 221

Query: 171 TSELFIDLIGKGGHAAFPHETKDMVVAASSLIMQLQTIVSRNVNP-LDSAVITIGKLTSG 229
           +  + I + G+  H A P    DM   A+ +I     I +R V+  +  +V+TI  +  G
Sbjct: 222 SDRITITVKGRQTHGARPWAGIDMASVAADIIQATNQIAARQVDVGVSPSVLTIATINMG 281

Query: 230 TVQNVIAERARLEGTIRTLSPEAMEKVKGRIEAIVRGIEVAYDCQAHIDYGSMYYQVYND 289
             QN+I E  ++EGT+RT S E  E +  R++  V  I   Y  +A + +   Y   YND
Sbjct: 282 FRQNIIPEDLKMEGTMRTFSKERREDLIARMQKSVTAIGDRYGAKAEVAFTQPYPVTYND 341

Query: 290 ETLTNEFMQFVEKETDVHLVRCQEAMTG-EDFGYMLARIPGFMFWLG 335
             L+      +EK +   +      +TG EDF     ++PG    LG
Sbjct: 342 PALSKWVKGTLEKASPGKVDDNAALVTGAEDFSMYAEKVPGVFIQLG 388


Lambda     K      H
   0.322    0.137    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 425
Number of extensions: 23
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 378
Length of database: 432
Length adjustment: 31
Effective length of query: 347
Effective length of database: 401
Effective search space:   139147
Effective search space used:   139147
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis