GapMind for Amino acid biosynthesis

 

Protein Echvi_2000 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Echvi_2000 aspartate kinase

Length: 815 amino acids

Source: Cola in FitnessBrowser

Candidate for 6 steps in Amino acid biosynthesis

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-methionine biosynthesis hom hi Aspartokinase; EC 1.1.1.3; EC 2.7.2.4 (characterized, see rationale) 45% 100% 707.6 aspartate kinase (EC 2.7.2.4) 37% 308.1
L-threonine biosynthesis hom hi Aspartokinase; EC 1.1.1.3; EC 2.7.2.4 (characterized, see rationale) 45% 100% 707.6 aspartate kinase (EC 2.7.2.4) 37% 308.1
chorismate biosynthesis asp-kinase med Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic; AK-HD 1; AK-HSDH 1; Beta-aspartyl phosphate homoserine 1; EC 2.7.2.4; EC 1.1.1.3 (characterized) 40% 90% 587.4
chorismate biosynthesis asp-kinase hi aspartate kinase (EC 2.7.2.4) (TIGR00657) 99% 393.8
L-lysine biosynthesis asp-kinase med Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic; AK-HD 1; AK-HSDH 1; Beta-aspartyl phosphate homoserine 1; EC 2.7.2.4; EC 1.1.1.3 (characterized) 40% 90% 587.4
L-lysine biosynthesis asp-kinase hi aspartate kinase (EC 2.7.2.4) (TIGR00657) 99% 393.8
L-methionine biosynthesis asp-kinase med Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic; AK-HD 1; AK-HSDH 1; Beta-aspartyl phosphate homoserine 1; EC 2.7.2.4; EC 1.1.1.3 (characterized) 40% 90% 587.4
L-methionine biosynthesis asp-kinase hi aspartate kinase (EC 2.7.2.4) (TIGR00657) 99% 393.8
L-threonine biosynthesis asp-kinase med Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic; AK-HD 1; AK-HSDH 1; Beta-aspartyl phosphate homoserine 1; EC 2.7.2.4; EC 1.1.1.3 (characterized) 40% 90% 587.4
L-threonine biosynthesis asp-kinase hi aspartate kinase (EC 2.7.2.4) (TIGR00657) 99% 393.8

Sequence Analysis Tools

View Echvi_2000 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MKIIKFGGSSIANYENIQKVFSIIEQKSEKEAFALVFSAFGGVTEQLLQCANIAQQSEES
YHTILQELEKRHLEIVKKLVPVQQQSTALTFVKVRFNELGDLFHGIYLIKECSNRTMDYV
LSFGERLSNFILAAGLQAKGIGTSYVDARDLVKTDDRFGHAKVNFKTTNKLIQDHFKSHD
DIKVITGFIGSTEKGETTTVGRSGSDYTASIFAAALGAEQVEIWTDVSGVMTADPRLVYT
AFTIPQLSYNEAMELSHFGAKVVFPATMQPAMKEDIPIYIKNTFKPDEAGTRISKDSGEG
KIIKGISSMDNISILNVQGPGLVEVVGVSQRFFGTLANNGINIILISQASSEHSICVAIA
SKDASRAKSVIEEEFRYEIQSGEMDEIQIVPDMAVIAVVGENMQHNPGASGRMFQALGRN
NVNVAAIAQGSSELNISAVITQADLQKALNALHEAFFLSDYKVLHLFLVGVGLIGKALTK
MIHQQLKNLQEENMLDIQIHGMANSRYMKFHEDGFDLATVGPPDENDEPMDMDKFIGTMT
EMNFSNSVFVDCTASQDVADIYEQILDSKVGIVTPNKKANSGPLETYKKLKKLAGQRGVR
FFYETNVAAGLPVINTLQDLMLSGDHVHRIEAVLSGSMNYIFSELEKGMPFSEVVAQAKE
KGYTEPDPRDDLSGMDVARKILILGREAGQDLHFEDVEIQSMVPEDCEDAASVPEFFKKL
QKHDGHFQQLLDEANAKGEKLRFMATLENGKAKVGLNSLDSEHPFFTLKGSDNMILFTTE
RYNDFPMIVRGPGAGADVTAAGVFADIIRLGNYSR

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory