Align Carbamoyl-phosphate synthase small chain; Carbamoyl-phosphate synthetase glutamine chain; EC 6.3.5.5 (characterized)
to candidate RR42_RS13520 RR42_RS13520 carbamoyl phosphate synthase small subunit
Query= SwissProt::P0A6F1 (382 letters) >FitnessBrowser__Cup4G11:RR42_RS13520 Length = 378 Score = 509 bits (1311), Expect = e-149 Identities = 247/376 (65%), Positives = 296/376 (78%), Gaps = 4/376 (1%) Query: 4 SALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNV 63 SA+L L DGT F G +IGA+G +GEVVFNT++TGYQEILTDPSYSRQIVTLTYPHIGNV Sbjct: 7 SAILALADGTVFRGYSIGASGHTIGEVVFNTALTGYQEILTDPSYSRQIVTLTYPHIGNV 66 Query: 64 GTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYLKRHNIVAIADIDTRKLTRLLR 123 G N D E+++VHA GL+I+DLPL+ASNFR L YLK+ +VAIA IDTRKLTR+LR Sbjct: 67 GVNTEDVEATKVHAAGLIIKDLPLLASNFRKEHSLGHYLKQEKVVAIAGIDTRKLTRILR 126 Query: 124 EKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAKEVTTAEAYSWTQGSWTLTGGLPE 183 EKGAQNGCI+AG++ + A++ AR+FPGL GMDLAK V+ E Y W Q W L G Sbjct: 127 EKGAQNGCILAGED-NVQKAIDLARSFPGLAGMDLAKVVSVKEPYEWNQTEWVLGQGYG- 184 Query: 184 AKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTIVPAQTSAEDVLKMNPDGIFLSNGPG 243 K + FHVVA+D+G K NILRML RGC++T++PAQ SAED L +NPDG+FLSNGPG Sbjct: 185 --KLESPRFHVVAFDYGVKFNILRMLAARGCKVTVLPAQASAEDALALNPDGVFLSNGPG 242 Query: 244 DPAPCDYAITAIQKFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDVEKN 303 DP PCDYAI A ++F+ IP FGICLGHQ++ALA+GAKT+KMKFGHHG NHPVKD++ Sbjct: 243 DPEPCDYAIRATREFIARGIPTFGICLGHQIMALAAGAKTLKMKFGHHGANHPVKDLDDG 302 Query: 304 VVMITAQNHGFAVDEATLPANLRVTHKSLFDGTLQGIHRTDKPAFSFQGHPEASPGPHDA 363 V+IT+QNHGFAVD TLPAN+R TH SLFDG+LQG TDKPAF FQGHPEASPGPHD Sbjct: 303 RVVITSQNHGFAVDADTLPANVRATHVSLFDGSLQGFALTDKPAFCFQGHPEASPGPHDV 362 Query: 364 APLFDHFIELIEQYRK 379 A LFD F +++ R+ Sbjct: 363 AYLFDRFTKMMADARQ 378 Lambda K H 0.318 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 521 Number of extensions: 13 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 382 Length of database: 378 Length adjustment: 30 Effective length of query: 352 Effective length of database: 348 Effective search space: 122496 Effective search space used: 122496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate RR42_RS13520 RR42_RS13520 (carbamoyl phosphate synthase small subunit)
to HMM TIGR01368 (carA: carbamoyl-phosphate synthase, small subunit (EC 6.3.5.5))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01368.hmm # target sequence database: /tmp/gapView.22438.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01368 [M=361] Accession: TIGR01368 Description: CPSaseIIsmall: carbamoyl-phosphate synthase, small subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.3e-149 482.7 0.0 3.7e-149 482.5 0.0 1.0 1 lcl|FitnessBrowser__Cup4G11:RR42_RS13520 RR42_RS13520 carbamoyl phosphate Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Cup4G11:RR42_RS13520 RR42_RS13520 carbamoyl phosphate synthase small subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 482.5 0.0 3.7e-149 3.7e-149 1 360 [. 8 374 .. 8 375 .. 0.97 Alignments for each domain: == domain 1 score: 482.5 bits; conditional E-value: 3.7e-149 TIGR01368 1 atlvledGtvfegksfgaekevvGevvFnTsmtGYqEiltDpsYkgqivvltyplignygvneedaesk 69 a+l+l+dGtvf+g+s+ga+++++GevvFnT++tGYqEiltDpsY++qiv+ltyp+ign+gvn+ed+e++ lcl|FitnessBrowser__Cup4G11:RR42_RS13520 8 AILALADGTVFRGYSIGASGHTIGEVVFNTALTGYQEILTDPSYSRQIVTLTYPHIGNVGVNTEDVEAT 76 689****************************************************************** PP TIGR01368 70 kikvkglvvkelskevsnyrakesLeeflkeegivaiegvDTRalvkklRekgsmkavisteksekeel 138 k++++gl++k+l +sn+r+++sL ++lk+e++vai+g+DTR+l++ lRekg+++++i + + + +++ lcl|FitnessBrowser__Cup4G11:RR42_RS13520 77 KVHAAGLIIKDLPLLASNFRKEHSLGHYLKQEKVVAIAGIDTRKLTRILREKGAQNGCILAGEDNVQKA 145 ********************************************************************* PP TIGR01368 139 vekakespkvkevnlvkevstkeayeleq......k..akkegkklrvvvidlGvKenilreLvkrgve 199 ++ a++ p +++++l+k vs+ke+ye++q + k e+ + +vv++d+GvK nilr+L++rg++ lcl|FitnessBrowser__Cup4G11:RR42_RS13520 146 IDLARSFPGLAGMDLAKVVSVKEPYEWNQtewvlgQgyGKLESPRFHVVAFDYGVKFNILRMLAARGCK 214 ****************************966655526567788889*********************** PP TIGR01368 200 vtvvpadtsaeeikklnpdgillsnGPGdPaaveeaietvkklleakiPifGIclGhqllalalgakty 268 vtv+pa++sae+ +lnpdg++lsnGPGdP+ +++ai+ +++++ + iP+fGIclGhq++ala+gakt+ lcl|FitnessBrowser__Cup4G11:RR42_RS13520 215 VTVLPAQASAEDALALNPDGVFLSNGPGDPEPCDYAIRATREFIARGIPTFGICLGHQIMALAAGAKTL 283 ********************************************************************* PP TIGR01368 269 klkfGhrGaNhpvkdlktgrveitsqNHgyavdeeslkeeelevthvnlnDgtveglehkelpvfsvQy 337 k+kfGh+GaNhpvkdl++grv itsqNHg+avd ++l+++ +++thv+l+Dg+++g + +++p+f Q lcl|FitnessBrowser__Cup4G11:RR42_RS13520 284 KMKFGHHGANHPVKDLDDGRVVITSQNHGFAVDADTLPAN-VRATHVSLFDGSLQGFALTDKPAFCFQG 351 ************************************8866.**************************** PP TIGR01368 338 HPeaspGphdteylFdefvelik 360 HPeaspGphd +ylFd+f+++++ lcl|FitnessBrowser__Cup4G11:RR42_RS13520 352 HPEASPGPHDVAYLFDRFTKMMA 374 *******************9986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (361 nodes) Target sequences: 1 (378 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.84 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory