Align Isocitrate lyase (EC 4.1.3.1) (characterized)
to candidate RR42_RS25845 RR42_RS25845 isocitrate lyase
Query= reanno::psRCH2:GFF1971 (531 letters) >FitnessBrowser__Cup4G11:RR42_RS25845 Length = 526 Score = 881 bits (2276), Expect = 0.0 Identities = 436/530 (82%), Positives = 475/530 (89%), Gaps = 4/530 (0%) Query: 1 MSAYQDDIKAVAALKEQYGSSWAAINPESVARMRAQNRFKTGLEIAQYTADIMRKDMAEY 60 M+ YQDDIKAVA LKE +GS+W AINPE ARMRAQN+FKTGL+IA+YTA IMR DMA Y Sbjct: 1 MAQYQDDIKAVAGLKENHGSAWNAINPEYAARMRAQNKFKTGLDIAKYTAKIMRADMAAY 60 Query: 61 DADSSVYTQSLGCWHGFIGQQKLISIKKHLKTTNKRYLYLSGWMVAALRSEFGPLPDQSM 120 DADSS YTQSLGCWHGFIGQQK+ISIKKH +T +RYLYLSGWMVAALRSEFGPLPDQSM Sbjct: 61 DADSSKYTQSLGCWHGFIGQQKMISIKKHFNSTERRYLYLSGWMVAALRSEFGPLPDQSM 120 Query: 121 HEKTAVSDLIEELYTFLRQADSRELDLLFTALDAAREAGDNAKAAEIQNQIDNYETHIVP 180 HEKT+VS LI ELYTFLRQAD+REL LF LDAA+ AKAA IQ +IDN+ TH+VP Sbjct: 121 HEKTSVSALIRELYTFLRQADARELGGLFRELDAAQGP---AKAA-IQEKIDNHVTHVVP 176 Query: 181 IIADIDAGFGNPEATYLLAKRMIEAGACCIQIENQVSDEKQCGHQDGKVTVPHADFLAKI 240 IIADIDAGFGN EATYLLAK+ IEAGACCIQIENQVSDEKQCGHQDGKVTVPH DFLAKI Sbjct: 177 IIADIDAGFGNAEATYLLAKQFIEAGACCIQIENQVSDEKQCGHQDGKVTVPHEDFLAKI 236 Query: 241 AAVRYAFLELGIDNGVIVARTDSLGAGLTKQIAVTKEPGDLGDLYNSFLDCEEVSEAALG 300 A+RYAFLELG+D+GVIVARTDSLGAGLTKQIAVT GDLGD YNSFLDCEE+S LG Sbjct: 237 RAIRYAFLELGVDDGVIVARTDSLGAGLTKQIAVTNTTGDLGDQYNSFLDCEELSADELG 296 Query: 301 NGDVVIKREGKLLRPKRLPSNLFQFRAGTGEARCILDCITSLQNGADLLWIETEKPHVGQ 360 NGDV+IKR+GKLLRPKRLPSNLFQFRAGTGEARC+LDC+T+LQNGADLLWIETEKPH+ Q Sbjct: 297 NGDVIIKRDGKLLRPKRLPSNLFQFRAGTGEARCVLDCVTALQNGADLLWIETEKPHIAQ 356 Query: 361 IAAMVDEIRKTIPNAKLVYNNSPSFNWTLNFRQQVFDAFVAEGKDVSAYDRAKLMSVEYD 420 I MV EIRK IPNAKLVYNNSPSFNWTLNFRQQV+DA A GKDVSAY+R +LM+VEYD Sbjct: 357 IGGMVSEIRKVIPNAKLVYNNSPSFNWTLNFRQQVYDAMKAAGKDVSAYERTQLMNVEYD 416 Query: 421 ETELAQVADEKIRTFQRDGSAHAGIFHHLITLPTYHTAALSTDNLAKGYFADQGMLAYVK 480 +TELA++ADEKIRTFQ D S AGIFHHLITLPTYHTAALSTDNLAK YF DQGML YV Sbjct: 417 QTELAKLADEKIRTFQADSSREAGIFHHLITLPTYHTAALSTDNLAKEYFGDQGMLGYVA 476 Query: 481 GVQRQELRQGIACVKHQNMAGSDIGDNHKEYFAGEAALKASGKDNTMNQF 530 GVQR+E+RQGIACVKHQNM+GSDIGD+HKEYF+GEAALKA+GKDNTMNQF Sbjct: 477 GVQRKEIRQGIACVKHQNMSGSDIGDDHKEYFSGEAALKAAGKDNTMNQF 526 Lambda K H 0.318 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 957 Number of extensions: 35 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 531 Length of database: 526 Length adjustment: 35 Effective length of query: 496 Effective length of database: 491 Effective search space: 243536 Effective search space used: 243536 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory