Alignments for a candidate for ilvI in Cupriavidus basilensis 4G11

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Cupriavidus basilensis 4G11

Align acetolactate synthase (EC 2.2.1.6) (characterized)
to candidate RR42_RS02175 RR42_RS02175 acetolactate synthase

Query= BRENDA::P9WG39
         (547 letters)



>FitnessBrowser__Cup4G11:RR42_RS02175
          Length = 541

 Score =  253 bits (646), Expect = 1e-71
 Identities = 177/539 (32%), Positives = 259/539 (48%), Gaps = 26/539 (4%)

Query: 8   AQTMHAGRLIARRLKASGIDTVFTLSGGHLFSIYDGCREEGIRLIDTRHEQTAAFAAEGW 67
           A++      +A  L  +G+  +FTLSG H+  ++D C E  I LI TRHE      A+ W
Sbjct: 2   AKSHRGADALAGTLAKAGVRRIFTLSGNHIMPVFDACLEAEIALIHTRHEAATVHMADAW 61

Query: 68  SKVTRVPGVAALTAGPGITNGMSAMAAAQQNQSPLVVLGGRAPALRWGMGSLQEIDHVPF 127
           S++T   G+A +T GPG  N +SA+  AQ +++PLV+L G AP  + G G+ QE+     
Sbjct: 62  SRITGEVGMAMVTGGPGHANAVSALYTAQMSEAPLVLLSGHAPNSQRGFGAFQEMRQAEM 121

Query: 128 VAPVARFAATAQSAENAGLLVDQALQAAVSAPSGVAFVDFPMDHAFSMSSDNGRPGALTE 187
            APV + +  + SA+     +  A++ A S   G   +  P D   +  +  G P ++  
Sbjct: 122 AAPVTKASWASASADTVAADIALAMRIAQSGRPGPVHLSLPTDALENAVTRAGEPASIAA 181

Query: 188 LPAGPTPAGDALDRAAGLLSTAQRPVIMAGTNVWWGHAEAALLRLVEERHIPVLMNGMAR 247
               P             L+ A+RP+I+AG         +    L     IPV+     R
Sbjct: 182 PQEAPAFDARTASEVLAALAAAKRPLILAGPAWLTASGRSRTAELEARTGIPVVGMESPR 241

Query: 248 GVVPADHRLAFSRARSKALGEADVALIVGVPMDFRLGFG--GVFGSTTQLIVADRVEPAR 305
           G    D  L    A  + L +AD  L++G  +DF L FG    F +    +  D  +   
Sbjct: 242 G--NNDPSLG---AFGQMLAQADCILLLGKRLDFTLKFGRPPAFAADCNFLQIDADDAEL 296

Query: 306 EHPRPVAAGL--------YGDLTATLSALAGSGGTDHQGWIEELATAETMARDLEKAELV 357
           E  R  A            G   A L A A +G   H  W+E++  A     +  K    
Sbjct: 297 ERSRRAAGARVLLARRADVGSAIAQLLACA-TGREQHCAWLEDVKAAIRYRPEAWKT--A 353

Query: 358 DDRIPLHPMRVYAELAALLER--DALVVIDAGDFGSYAGRMIDSYLPGCWLDSGPFGCLG 415
             R  LHP +V + L  +LE   +A++V D G+FG +A   + +      + +GP G +G
Sbjct: 354 SSRGALHPAQVCSPLQPILESHPEAVLVSDGGEFGQWAQACLQA---PHRVINGPAGAIG 410

Query: 416 SGPGYALAAKLARPQRQVVLLQGDGAFGFSGMEWDTLVRHNVAVVSVIGNNGIWGLEKHP 475
           S    ALAA+LA+P   V+ + GDG  GF   E+DT VR+ +  V+V+GN+  W  E   
Sbjct: 411 SALPMALAARLAKPDAPVIAMMGDGTTGFHIAEFDTAVRYGLPFVAVVGNDARWNAEYQI 470

Query: 476 MEALYGYS--VVAELRPGTRYDEVVRALGGHGELVSVPAELRPALERAFASGLPAVVNV 532
               YG    V  EL P TRYD+V  A GG+GE V  P +L PA++RA ASG PA VNV
Sbjct: 471 QLNSYGKDRLVGCELLP-TRYDQVCAAFGGYGEFVQAPEQLLPAVQRAIASGRPACVNV 528


Lambda     K      H
   0.319    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 760
Number of extensions: 43
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 547
Length of database: 541
Length adjustment: 35
Effective length of query: 512
Effective length of database: 506
Effective search space:   259072
Effective search space used:   259072
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis