Align 3-isopropylmalate dehydrogenase; 3-IPM-DH; Beta-IPM dehydrogenase; IMDH; EC 1.1.1.85 (characterized)
to candidate RR42_RS14385 RR42_RS14385 3-isopropylmalate dehydrogenase
Query= SwissProt::Q56268 (358 letters) >FitnessBrowser__Cup4G11:RR42_RS14385 Length = 355 Score = 407 bits (1045), Expect = e-118 Identities = 218/358 (60%), Positives = 263/358 (73%), Gaps = 10/358 (2%) Query: 3 KIAIFAGDGIGPEIVAAARQVLDAVDQAAHLGLRCTEGLVGGAALDASDDPLPAASLQLA 62 KIA+ GDGIGPEI+A A +VL+A+D+ + VGGA +AS PLP A+L+LA Sbjct: 2 KIAVLPGDGIGPEIIAEAVKVLNALDEKFEM----ETAPVGGAGYEASGHPLPDATLKLA 57 Query: 63 MAADAVILGAVGGPRWDAYPPAKRPEQGLLRLRKGLDLYANLRPAQIFPQLLDASPLRPE 122 ADA++ GAVG ++D RPEQ +L LRK L L+AN RPA +P+L AS L+PE Sbjct: 58 QEADAILFGAVGDWKYDKLDRPLRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPE 117 Query: 123 LVRDVDILVVRELTGDIYFGQPRGLEV-----IDGKRRGFNTMVYDEDEIRRIAHVAFRA 177 LV +DIL+VREL GDIYFGQPRG+ G R GF+TM Y E EIRRIAHVAF+A Sbjct: 118 LVAGLDILIVRELNGDIYFGQPRGMREAPDGPFKGAREGFDTMRYSEPEIRRIAHVAFQA 177 Query: 178 AQGRRKQLCSVDKANVLETTRLWREVVTEVARDYPDVRLSHMYVDNAAMQLIRAPAQFDV 237 A R K+LCSVDKANVLET + W++VV +V ++YP+V LSHMYVDNAAMQLI+AP FDV Sbjct: 178 AAKRGKKLCSVDKANVLETFQFWKDVVIDVHKEYPEVELSHMYVDNAAMQLIKAPKSFDV 237 Query: 238 LLTGNMFGDILSDEASQLTGSIGMLPSASL-GEGRAMYEPIHGSAPDIAGQDKANPLATI 296 ++TGNMFGDILSDEA+ LTGSIGMLPSASL + +YEP HGSAPDIAG+ ANPLATI Sbjct: 238 IVTGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATI 297 Query: 297 LSVAMMLRHSLNAEPWAQRVEAAVQRVLDQGLRTADIAAPGTPVIGTKAMGAAVVNAL 354 LS AMMLR SLN A R+E AV++VL QG RT DI PG +GT+ MGAAV+ AL Sbjct: 298 LSAAMMLRFSLNRAEQADRIENAVKKVLAQGYRTGDILTPGCKQVGTREMGAAVLAAL 355 Lambda K H 0.320 0.137 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 412 Number of extensions: 19 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 358 Length of database: 355 Length adjustment: 29 Effective length of query: 329 Effective length of database: 326 Effective search space: 107254 Effective search space used: 107254 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate RR42_RS14385 RR42_RS14385 (3-isopropylmalate dehydrogenase)
to HMM TIGR00169 (leuB: 3-isopropylmalate dehydrogenase (EC 1.1.1.85))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00169.hmm # target sequence database: /tmp/gapView.8903.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00169 [M=349] Accession: TIGR00169 Description: leuB: 3-isopropylmalate dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.8e-150 486.1 0.0 3.2e-150 485.9 0.0 1.0 1 lcl|FitnessBrowser__Cup4G11:RR42_RS14385 RR42_RS14385 3-isopropylmalate d Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Cup4G11:RR42_RS14385 RR42_RS14385 3-isopropylmalate dehydrogenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 485.9 0.0 3.2e-150 3.2e-150 1 347 [. 2 349 .. 2 351 .. 0.97 Alignments for each domain: == domain 1 score: 485.9 bits; conditional E-value: 3.2e-150 TIGR00169 1 kiavLpGDgiGpevvaealkvLkaveerfelklefeealiGGaaidatgePlpeetlkackeadavLlg 69 kiavLpGDgiGpe++aea+kvL+a++e+ +e+e+a +GGa +a g+Plp++tlk ++eada+L+g lcl|FitnessBrowser__Cup4G11:RR42_RS14385 2 KIAVLPGDGIGPEIIAEAVKVLNALDEK----FEMETAPVGGAGYEASGHPLPDATLKLAQEADAILFG 66 79***********************996....567899******************************* PP TIGR00169 70 avGGpkWdnlprdvrPekgLLklrkeldlfanLrPaklfksLeklsplkeeivkgvDlvvvreLtgGiY 138 avG k+d+l r rPe+++L lrk+l+lfan+rPa +++L+ +s+lk+e+v g+D+++vreL g iY lcl|FitnessBrowser__Cup4G11:RR42_RS14385 67 AVGDWKYDKLDRPLRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVAGLDILIVRELNGDIY 135 ********************************************************************* PP TIGR00169 139 fGepkereeaee.....ekkaldtekYtkeeieriarvafelarkrrkkvtsvDkanvLessrlWrktv 202 fG+p++++ea + ++++dt++Y+++ei+ria+vaf++a+kr kk++svDkanvLe+ ++W+++v lcl|FitnessBrowser__Cup4G11:RR42_RS14385 136 FGQPRGMREAPDgpfkgAREGFDTMRYSEPEIRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDVV 204 ******99974322324789************************************************* PP TIGR00169 203 eeiakeyPdvelehlyiDnaamqLvksPeqldvvvtsnlfGDilsDeasvitGslGlLPsaslsskgla 271 +++keyP+vel+h+y+DnaamqL+k+P+++dv+vt+n+fGDilsDea+++tGs+G+LPsasl + ++ lcl|FitnessBrowser__Cup4G11:RR42_RS14385 205 IDVHKEYPEVELSHMYVDNAAMQLIKAPKSFDVIVTGNMFGDILSDEAAMLTGSIGMLPSASLDANNKG 273 ********************************************************************* PP TIGR00169 272 lfepvhgsapdiagkgianpiaailsaalllryslnleeaaeaieaavkkvleegkrtedlaseattav 340 l+ep hgsapdiagkg+anp+a+ilsaa++lr sln e+a++ie+avkkvl++g+rt d+ + ++++v lcl|FitnessBrowser__Cup4G11:RR42_RS14385 274 LYEPSHGSAPDIAGKGVANPLATILSAAMMLRFSLNRAEQADRIENAVKKVLAQGYRTGDILTPGCKQV 342 ********************************************************************* PP TIGR00169 341 stkevee 347 +t+e++ lcl|FitnessBrowser__Cup4G11:RR42_RS14385 343 GTREMGA 349 ****986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (349 nodes) Target sequences: 1 (355 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 10.91 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory