Alignments for a candidate for ilvI in Cupriavidus basilensis 4G11

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Cupriavidus basilensis 4G11

Align Acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate RR42_RS29140 RR42_RS29140 thiamine pyrophosphate-binding protein

Query= curated2:Q7U5G1
         (617 letters)



>FitnessBrowser__Cup4G11:RR42_RS29140
          Length = 558

 Score =  259 bits (663), Expect = 2e-73
 Identities = 181/551 (32%), Positives = 264/551 (47%), Gaps = 27/551 (4%)

Query: 18  PQTISGAAALMDALRRHGVDTIFGYPGGAILPIYDALHIAESEGWVKHILVRHEQAGTHA 77
           P   SG   L+DALR HG   IF  PG + L + DALH    +  +  I+ +HE A  + 
Sbjct: 10  PAARSGGRVLVDALRIHGAQRIFCVPGESFLDVLDALH---DQPAIDLIVCKHEGAAANM 66

Query: 78  ADAYARATGKVGVCFGTSGPGATNLVTGIATAQMDSVPMVVITGQVPRPAIGTDAFQETD 137
           A+A  + TG+ G+CF T GPGAT+   G+  A  DS PM++  GQ+ R   G +AFQE D
Sbjct: 67  AEADGKLTGRPGICFVTRGPGATHASIGVHIAAQDSTPMLLFVGQIARGHKGREAFQEVD 126

Query: 138 IFGITLPIVKHSWVVRDPADLGSIVAQAFLIAASGRPGPVLIDIPKDVGQEQFNYVPVEP 197
              +   I K    + DPA +  +VA+AF  A SGRPGPV+I +P+DV          + 
Sbjct: 127 YGAMFGSIAKWVVEIEDPARIPELVARAFQCATSGRPGPVVISLPEDVLDGLCEV--ADT 184

Query: 198 GSVIPGGFHQPEPPLDAAVAAALDLIEQAQRPLLYVGGGAISACAHDSLRMLAERYQLPV 257
           G   P          DA  AA    +  A+RPL+  GG   SA A        +R+ LPV
Sbjct: 185 GRYRPVAAAPRAADADALAAA----LAGAERPLVIAGGANWSAQAAADFAAFVQRWNLPV 240

Query: 258 TTTLMGKGAFDENDALSVGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTG---KLDTF 314
                 +  FD  D   VG L +         V   D+++A G R  D  T     L+  
Sbjct: 241 ACAFRRQDVFDNRDPHYVGHLSLGVNPALAERVRTADVILAFGTRLGDIATDGYTLLEAP 300

Query: 315 APRARVVHFEIDPAEIGKNRKADVAVLGDLGLSLARMVEISLQRTAEPRTAAWLERINTW 374
            PR R+ H   D AE+G+  + ++A+   +    A +  ++      P    W +  +  
Sbjct: 301 QPRQRLFHLHADSAELGRVYQPELAIHAGIEPGAAMLAALT-----PPAAVRWGDWTSAA 355

Query: 375 KDRYPLTIPPAE------GAIYPQEVLLAVRDLAPDAIVTTDVGQHQMWAAQHLR-NGPR 427
           +  +   + PA+      G      V    R L  DA++T   G + +W  ++     PR
Sbjct: 356 RAAHGAFVAPAKPHPQLTGVDMGAVVAHLDRILPDDAVLTNGAGNYTVWLHRYYAYRQPR 415

Query: 428 GWISSAGLGTMGFGMPAAMGAQVAMPDRQVVCIAGDASILMNIQELGTLAAYGLPVKVVI 487
             ++    G MG+G+PAA+ A++  P R VVC AGD   LM  QEL T A YG  + VV+
Sbjct: 416 TELAPT-CGAMGYGLPAAVAAKLRDPQRTVVCFAGDGCFLMYPQELATAAQYGANLIVVV 474

Query: 488 VNNHWQGMVRQWQESFYDERYSASDMLNGMPDFIALARSFGVDGVKITDRELLHRDLAAA 547
           VNN   G +R  QE  Y  R S +D+ +  PDF+A+ARS G    ++T  E      A A
Sbjct: 475 VNNGMYGTIRMHQEKRYPGRVSGTDIPS--PDFVAMARSCGAWAERVTQTEDFAAAFARA 532

Query: 548 LQSPTPTMIDV 558
             +  P ++++
Sbjct: 533 QAAGKPAVLEL 543


Lambda     K      H
   0.320    0.136    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1013
Number of extensions: 53
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 617
Length of database: 558
Length adjustment: 37
Effective length of query: 580
Effective length of database: 521
Effective search space:   302180
Effective search space used:   302180
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis