Alignments for a candidate for lysT in Cupriavidus basilensis 4G11

GapMind for Amino acid biosynthesis

Alignments for a candidate for lysT in Cupriavidus basilensis 4G11

Align Homoaconitase large subunit; HACN; Homoaconitate hydratase; EC 4.2.1.36 (characterized)
to candidate RR42_RS01530 RR42_RS01530 isopropylmalate isomerase

Query= SwissProt::Q9ZNE0
         (418 letters)



>FitnessBrowser__Cup4G11:RR42_RS01530
          Length = 472

 Score =  224 bits (572), Expect = 3e-63
 Identities = 151/416 (36%), Positives = 213/416 (51%), Gaps = 60/416 (14%)

Query: 55  PERVSIVIDHVAPAANLEVAKAQ--------KEIREWGKRHGIRVFDVG---RGVCHQVL 103
           P +   V+DH+ P       + Q        + ++    +HGI +FD     +G+ H + 
Sbjct: 55  PGQQVAVVDHIIPTHAESPRRIQDPASALQARNLKRNCDQHGIPLFDTDDALQGIEHVIA 114

Query: 104 IEEGLAQPGWVVVGSDSHSTTYGAVGAFGTGMGATDIALAAASGRTWLRVPESVKVVFRG 163
            E G+ +PG VV+  DSH+TTYGA+GA G G+G +++    A+     RV   +++   G
Sbjct: 115 PEHGMIRPGMVVLCGDSHTTTYGALGALGFGIGTSEVEHVLATQTLVYRVAMDMRIRVDG 174

Query: 164 RLPKGVTAKDAALEMVRLLTAEGATYMAVEIHLLDGAEALTRGERMTLANLTVEAGAKAG 223
            LP G TAKD  L ++  ++A+GA    VE        AL+   RMTL N+TVEAGA+  
Sbjct: 175 VLPCGATAKDMILHIISRISAQGARGYVVEF-TGSAIAALSIEARMTLCNMTVEAGARGA 233

Query: 224 LVVPSGEILE--MYRVPD------------W--LYPDPDARYAKEVEIDLSALTPRVS-- 265
           L+ P    +E  + R  D            W  L+ D DAR+  E+  D S + P VS  
Sbjct: 234 LIAPDAAAIEYVLARAADIDATLRERALACWRALHSDADARFDSELVFDASNVAPYVSWG 293

Query: 266 ------------VPFYVDNVHEVAQ-----------------VKGKRVDQVFIGTCTNGR 296
                       VP   D + + A+                 ++G  V +VFIG+CTNGR
Sbjct: 294 TSPDQAIPIDACVPPVEDAIDDTARSAAAKALDYIGLQAGATLEGLPVQRVFIGSCTNGR 353

Query: 297 IEDLRAAAEVLRGRKVAPWVRLLVVPASSQVLEEAARDGTLLTLLEAGATIGTPGCGPCM 356
           IEDLRA A ++RGRKVA  VR +VVP S  V ++A  +G    LL+AG     PGC  C+
Sbjct: 354 IEDLRAVAAIVRGRKVAAGVRAMVVPGSGAVRQQAEDEGIAQVLLDAGFEWRKPGCSMCL 413

Query: 357 GRHMGVLAPGEVCVSTSNRNFRGRMGAPDAEIYLASPRVAAASAVAGYLTTPEELE 412
             +  VLAPGE C ST+NRNF GR G      +L SP +AAA+A+ G +T    LE
Sbjct: 414 AMNDDVLAPGERCASTTNRNFEGRQGRA-GRTHLMSPAMAAAAALTGCITDVRRLE 468


Lambda     K      H
   0.318    0.135    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 463
Number of extensions: 21
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 418
Length of database: 472
Length adjustment: 32
Effective length of query: 386
Effective length of database: 440
Effective search space:   169840
Effective search space used:   169840
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis