GapMind for Amino acid biosynthesis


Alignments for a candidate for lysU in Cupriavidus basilensis 4G11

Align Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC (characterized)
to candidate RR42_RS24180 RR42_RS24180 3-isopropylmalate dehydratase

Query= SwissProt::Q58667
         (170 letters)

          Length = 216

 Score = 64.7 bits (156), Expect = 1e-15
 Identities = 46/144 (31%), Positives = 68/144 (47%), Gaps = 31/144 (21%)

           +VDTDAIIP  +L++           D +        G+D            N P+  + 

             +++A  NFGCGSSRE A  A+   G  AVIA SFA IF+ N    GL+P++    ++ 

Query: 112 ---------DGDIVEIDLDKEEIV 126
                     G  + IDLDK+ ++

Lambda     K      H
   0.318    0.139    0.399 

Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 111
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 170
Length of database: 216
Length adjustment: 20
Effective length of query: 150
Effective length of database: 196
Effective search space:    29400
Effective search space used:    29400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 44 (21.6 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory