Alignments for a candidate for thrB in Cupriavidus basilensis 4G11

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrB in Cupriavidus basilensis 4G11

Align Homoserine kinase; HK; HSK; EC 2.7.1.39 (characterized)
to candidate RR42_RS15065 RR42_RS15065 serine kinase

Query= SwissProt::P29364
         (316 letters)



>FitnessBrowser__Cup4G11:RR42_RS15065
          Length = 327

 Score =  222 bits (566), Expect = 8e-63
 Identities = 135/327 (41%), Positives = 183/327 (55%), Gaps = 14/327 (4%)

Query: 1   MSVFTPLERSTLEAFLAPYDLGRLRDFRGIAEGSENSNFFVSLEHG----EFVLTLVERG 56
           M+VFT +    + ++L  Y+LG +RD RGIA G ENSNFF+++E      E+VLT+ ER 
Sbjct: 1   MAVFTTVSPDEIASWLLDYNLGEMRDLRGIASGIENSNFFLTMEQDGVTREYVLTIFERL 60

Query: 57  PVQDLPFFIELLDVLHEDGLPVPYALRTRDGEALRRLEGKPALLQPRLAGRHERQPNAHH 116
             + LP+++ L+  L    + VP  +  RDGE LR L+GKPA +  RL G  +  P    
Sbjct: 61  TFEQLPYYLHLMAHLAAHDIRVPAPIPGRDGEILRALKGKPATIVTRLPGGSQLAPGVGQ 120

Query: 117 CQEVGDLLGHLHAATRGRILERPSDRGLPWMLEQGANLAPRLPEQARALLAPALAEIAAL 176
           C +VGD+L  +H A +    ++P+ R LPW  +    + P L    +ALL   LA   A 
Sbjct: 121 CAQVGDMLARMHLAGQSYPRQQPNLRSLPWWQQTEGEILPFLDAGQQALLREELAHQTAF 180

Query: 177 --DAERPALPRANLHADLFRDNVLF-DGP----HLAGLIDFYNACSGWMLYDLAITLNDW 229
              A+  ALP    H DLFRDN LF DGP     L G  DFY A +   L+DLA+T+NDW
Sbjct: 181 FGGADYAALPGGPCHCDLFRDNALFEDGPAGQEKLGGFFDFYFAGNDKWLFDLAVTVNDW 240

Query: 230 CSN-TDGSLDPARARALLAAYANRRPFTALEAEHWPSMLRVACVRFWLSRLIAAEAFAGQ 288
           C +   G+LD  R +ALL AY   RP   +EA HW  MLR   +RFW+SRL         
Sbjct: 241 CIDLASGALDADRGQALLRAYHAVRPLGPIEARHWRDMLRAGALRFWISRLWDFYLPREA 300

Query: 289 DVL-IHDPAEFEIRLAQRQNVEIHLPF 314
           D+L  HDP  FE R+ +R+     LP+
Sbjct: 301 DMLQPHDPTHFE-RILRRRIEAAALPW 326


Lambda     K      H
   0.324    0.140    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 321
Number of extensions: 18
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 316
Length of database: 327
Length adjustment: 28
Effective length of query: 288
Effective length of database: 299
Effective search space:    86112
Effective search space used:    86112
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 48 (23.1 bits)

Align candidate RR42_RS15065 RR42_RS15065 (serine kinase)
to HMM TIGR00938 (thrB: homoserine kinase (EC 2.7.1.39))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00938.hmm
# target sequence database:        /tmp/gapView.7243.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00938  [M=307]
Accession:   TIGR00938
Description: thrB_alt: homoserine kinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    5.3e-98  314.1   0.0      6e-98  313.9   0.0    1.0  1  lcl|FitnessBrowser__Cup4G11:RR42_RS15065  RR42_RS15065 serine kinase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Cup4G11:RR42_RS15065  RR42_RS15065 serine kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  313.9   0.0     6e-98     6e-98       1     306 [.       1     316 [.       1     317 [. 0.95

  Alignments for each domain:
  == domain 1  score: 313.9 bits;  conditional E-value: 6e-98
                                 TIGR00938   1 mavytsvsdeeleafLegydlGellslkGiaeGvensnyllttdk....gryvLtlyekrvkaeeLPff 65 
                                               mav+t+vs +e+ ++L  y+lGe+ +l+Gia+G+ensn++lt ++    + yvLt++e+ +  e+LP++
  lcl|FitnessBrowser__Cup4G11:RR42_RS15065   1 MAVFTTVSPDEIASWLLDYNLGEMRDLRGIASGIENSNFFLTMEQdgvtREYVLTIFERLT-FEQLPYY 68 
                                               9*****************************************985444479*********9.******* PP

                                 TIGR00938  66 lellthLaerglpvakpvksrdGralseLaGkPaalvefLkGssvakPtaercrevgevlaklhlagad 134
                                               l+l+ hLa++++ v++p++ rdG+ l  L+GkPa++v  L+G s   P + +c++vg++la++hlag++
  lcl|FitnessBrowser__Cup4G11:RR42_RS15065  69 LHLMAHLAAHDIRVPAPIPGRDGEILRALKGKPATIVTRLPGGSQLAPGVGQCAQVGDMLARMHLAGQS 137
                                               ********************************************************************* PP

                                 TIGR00938 135 fkeerkndlrleaWsilaakkfkvleqleeelaalldkeldalkkflp....rdLPrgvihadlfkdnv 199
                                               ++ ++ n lr   W+ +++ +  +l++l+++++all++el++ + f++      LP g  h dlf+dn 
  lcl|FitnessBrowser__Cup4G11:RR42_RS15065 138 YPRQQPN-LRSLPWWQQTEGE--ILPFLDAGQQALLREELAHQTAFFGgadyAALPGGPCHCDLFRDNA 203
                                               *******.**********998..*******************999976333367*************** PP

                                 TIGR00938 200 lld....g.dklkgvidfyfaCedallydlaiavndWcfe.addkldaaaakallkgyeavrpLseeek 262
                                               l++    g +kl+g+ dfyfa +d +l+dla++vndWc++ a + lda++ +all +y+avrpL ++e 
  lcl|FitnessBrowser__Cup4G11:RR42_RS15065 204 LFEdgpaGqEKLGGFFDFYFAGNDKWLFDLAVTVNDWCIDlASGALDADRGQALLRAYHAVRPLGPIEA 272
                                               **844432246****************************96789************************* PP

                                 TIGR00938 263 aafpvllrgaalrfllsrlldlvftqagelvvakdPaeferkLk 306
                                                 +  +lr++alrf++srl+d++ ++  ++ ++ dP++fer+L+
  lcl|FitnessBrowser__Cup4G11:RR42_RS15065 273 RHWRDMLRAGALRFWISRLWDFYLPREADMLQPHDPTHFERILR 316
                                               ******************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (307 nodes)
Target sequences:                          1  (327 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 10.64
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis