Alignments for a candidate for argI in Dinoroseobacter shibae DFL-12

GapMind for Amino acid biosynthesis

Alignments for a candidate for argI in Dinoroseobacter shibae DFL-12

Align Ornithine carbamoyltransferase, catabolic; OTCase; EC 2.1.3.3 (characterized)
to candidate 3607012 Dshi_0433 ornithine carbamoyltransferase (RefSeq)

Query= SwissProt::P08308
         (336 letters)



>FitnessBrowser__Dino:3607012
          Length = 333

 Score =  439 bits (1129), Expect = e-128
 Identities = 213/333 (63%), Positives = 268/333 (80%), Gaps = 2/333 (0%)

Query: 1   MAFNMHNRNLLSLMHHSTRELRYLLDLSRDLKRAKYTGTEQQHLKRKNIALIFEKTSTRT 60
           MAFN+ NR+ L+L   +  E+ +LL LS DLK AKY GTE   L+ K IALIFEK STRT
Sbjct: 1   MAFNLKNRHFLTLRGFTPEEIGFLLKLSADLKSAKYAGTEVPTLRGKEIALIFEKDSTRT 60

Query: 61  RCAFEVAAYDQGANVTYIDPNSSQIGHKESMKDTARVLGRMYDAIEYRGFKQEIVEELAK 120
           R  FEVAA+DQGA VTY+ P+ + +G KE++KDTARVLGR+YDAIEYRGF QEIV+ELA 
Sbjct: 61  RVGFEVAAHDQGATVTYLGPSGTHLGKKETVKDTARVLGRVYDAIEYRGFGQEIVQELAD 120

Query: 121 FAGVPVFNGLTDEYHPTQMLADVLTMREHSDKPLHDISYAYLGDARNNMGNSLLLIGAKL 180
           +AGVPV+NGLTDE+HPTQ+LAD LTM+EH +KPL +++Y ++GDA NNMG+SLL+ GAK+
Sbjct: 121 WAGVPVYNGLTDEFHPTQILADFLTMQEHCEKPLREVAYCFMGDAGNNMGDSLLIGGAKM 180

Query: 181 GMDVRIAAPKALWPHDEFVAQCKKFAEESGAKLTLTEDPKEAVKGVDFVHTDVWVSMGEP 240
           GMDVR+ AP++LWP      + +  AE +GA++TLTED  +AVKGVDFV+TDVWVSMGEP
Sbjct: 181 GMDVRLCAPRSLWPVQSIQDEARAIAEHTGARVTLTEDVDKAVKGVDFVYTDVWVSMGEP 240

Query: 241 VEAWGERIKELLPYQVNMEIMKATGNPRAKFMHCLPAFHNSETKVGKQIAEQYPNLANGI 300
           VE W ERI  L+PYQVN ++M  TGNPR +FMHCLPAFHN+ET+VG  I +++      +
Sbjct: 241 VEKWAERIDLLMPYQVNADVMARTGNPRVRFMHCLPAFHNAETEVGCDIQDKFG--LEAM 298

Query: 301 EVTEDVFESPYNIAFEQAENRMHTIKAILVSTL 333
           EVTE+VFESP +I F+QAENRMHTIKA+LV+TL
Sbjct: 299 EVTEEVFESPASIVFDQAENRMHTIKAVLVATL 331


Lambda     K      H
   0.318    0.133    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 396
Number of extensions: 10
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 336
Length of database: 333
Length adjustment: 28
Effective length of query: 308
Effective length of database: 305
Effective search space:    93940
Effective search space used:    93940
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate 3607012 Dshi_0433 (ornithine carbamoyltransferase (RefSeq))
to HMM TIGR00658 (argF: ornithine carbamoyltransferase (EC 2.1.3.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00658.hmm
# target sequence database:        /tmp/gapView.21726.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00658  [M=304]
Accession:   TIGR00658
Description: orni_carb_tr: ornithine carbamoyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
   2.3e-130  420.2   0.0   2.6e-130  420.0   0.0    1.0  1  lcl|FitnessBrowser__Dino:3607012  Dshi_0433 ornithine carbamoyltra


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Dino:3607012  Dshi_0433 ornithine carbamoyltransferase (RefSeq)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  420.0   0.0  2.6e-130  2.6e-130       1     303 [.       8     331 ..       8     332 .. 0.99

  Alignments for each domain:
  == domain 1  score: 420.0 bits;  conditional E-value: 2.6e-130
                         TIGR00658   1 rhllslldlseeelkellelakklkkekkkgkeekklkgktlaliFekrstRtRvsfevaayelGaqvlylnkeelq 77 
                                       rh+l+l  +++ee+  ll+l+++lk++k++g+e  +l+gk++aliFek+stRtRv fevaa ++Ga v+yl+++ ++
  lcl|FitnessBrowser__Dino:3607012   8 RHFLTLRGFTPEEIGFLLKLSADLKSAKYAGTEVPTLRGKEIALIFEKDSTRTRVGFEVAAHDQGATVTYLGPSGTH 84 
                                       8**************************************************************************** PP

                         TIGR00658  78 lgrkesikDtarvlsryvdaivvRvykhedveelakyasvPvingLtdlehPcqilaDlltikeklg.klkevklvy 153
                                       lg+ke++kDtarvl+r++dai +R++ +e+v+ela+ a+vPv+ngLtd++hP+qilaD+lt++e+++  l ev + +
  lcl|FitnessBrowser__Dino:3607012  85 LGKKETVKDTARVLGRVYDAIEYRGFGQEIVQELADWAGVPVYNGLTDEFHPTQILADFLTMQEHCEkPLREVAYCF 161
                                       *******************************************************************99******** PP

                         TIGR00658 154 vGDa.nnvanslllaaaklGldvvvatPeglepeaeivkkakkiakenggkleltedpkkavkdadviytDvwvsmG 229
                                       +GDa nn+++sll+++ak+G+dv++++P+ l+p ++i ++a++ia+++g++++lted++kavk++d++ytDvwvsmG
  lcl|FitnessBrowser__Dino:3607012 162 MGDAgNNMGDSLLIGGAKMGMDVRLCAPRSLWPVQSIQDEARAIAEHTGARVTLTEDVDKAVKGVDFVYTDVWVSMG 238
                                       ***99************************************************************************ PP

                         TIGR00658 230 e.eekkeerlkllkpyqvneellela.kpevkflhCLPavr.................Geevtdevlegeasivfde 287
                                       e  ek++er++ll pyqvn ++++ + +p v f+hCLPa++                  +evt+ev+e++asivfd+
  lcl|FitnessBrowser__Dino:3607012 239 EpVEKWAERIDLLMPYQVNADVMARTgNPRVRFMHCLPAFHnaetevgcdiqdkfgleAMEVTEEVFESPASIVFDQ 315
                                       *999**********************9****************************9999****************** PP

                         TIGR00658 288 aenRlhaqkavlkall 303
                                       aenR+h++kavl+a+l
  lcl|FitnessBrowser__Dino:3607012 316 AENRMHTIKAVLVATL 331
                                       *************998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (333 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 11.66
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis