Alignments for a candidate for gatB in Dinoroseobacter shibae DFL-12

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatB in Dinoroseobacter shibae DFL-12

Align Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.- (characterized)
to candidate 3607799 Dshi_1207 glutamyl-tRNA(Gln) amidotransferase, B subunit (RefSeq)

Query= SwissProt::Q9RF06
         (475 letters)



>FitnessBrowser__Dino:3607799
          Length = 505

 Score =  395 bits (1016), Expect = e-114
 Identities = 208/483 (43%), Positives = 303/483 (62%), Gaps = 14/483 (2%)

Query: 3   FETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAMRA 62
           +E VIGLEVH ++ T++K+FS +   FGAEPNSN   +D   PG+LPV+N+  V  A+R 
Sbjct: 21  WELVIGLEVHAQVATNAKLFSGASTLFGAEPNSNVAFVDAGMPGMLPVINEECVAQAVRT 80

Query: 63  AMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGENGYIDIEV-DGETKRIGITRL 121
            + L   I   S FDRKNYFYPD P+ YQISQ   PI   G + +E+ DG  + + I R+
Sbjct: 81  GLGLKAAINLTSAFDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVELGDGTARVVRIERI 140

Query: 122 HMEEDAGKSTHKGE--YSLVDLNRQGTPLIEIVSEPDIRSPKEAYAYLEKLRSIIQYTGV 179
           H+E+DAGKS H  +   S VDLNR G  L+EIVS PDIR P+EA AY+ KLR I++Y G 
Sbjct: 141 HLEQDAGKSIHDMDPHMSFVDLNRTGVALMEIVSRPDIRGPEEAAAYVSKLRQIMRYLGT 200

Query: 180 SDVKMEEGSLRCDANISL-RPYGQEKF---------GTKAELKNLNSFNYVRKGLEYEEK 229
            D  M+ G+LR D N+S+ RP   EK+         GT+ E+KN+NS  ++++ +EYE +
Sbjct: 201 CDGNMQNGNLRADVNVSVCRPGDYEKYQASQDFSHLGTRCEIKNMNSLRFIQQAIEYEAR 260

Query: 230 RQEEELLSGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKERVR 289
           RQ   +  GG + QETR +D   G+T  MR KE + DYRYFP+PD++PL I+ AW + + 
Sbjct: 261 RQIAIIEDGGSVTQETRLYDPDKGETRSMRSKEEAHDYRYFPDPDLLPLEIEQAWVDDIA 320

Query: 290 QTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVN 349
             +PELPD +KA+++ E GL  YDA VLT     + +FE+      D KL +NW++  + 
Sbjct: 321 AALPELPDAKKARFITEFGLSDYDASVLTADTTNAAYFEAVAGEAGDGKLAANWVINELF 380

Query: 350 EYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNGL 409
             L K   ++ ++ ++P  LAG+IKLI+   +S KIAK +F  +  +GG+  +I+E  G+
Sbjct: 381 GRLKKEDHDITESPVSPAQLAGIIKLIKADAISGKIAKDLFEIVYTEGGDPAEIVEARGM 440

Query: 410 VQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLK 469
            Q++D   +   V+E +  N   V   +    K  G+ VGQ+MKA+ G+ANP+ VNQ++ 
Sbjct: 441 KQVTDTGAIEAAVDEIIAANPAQVAKAQENP-KLAGWFVGQVMKATGGKANPKAVNQIVA 499

Query: 470 QEL 472
            +L
Sbjct: 500 AKL 502


Lambda     K      H
   0.313    0.133    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 638
Number of extensions: 28
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 475
Length of database: 505
Length adjustment: 34
Effective length of query: 441
Effective length of database: 471
Effective search space:   207711
Effective search space used:   207711
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)

Align candidate 3607799 Dshi_1207 (glutamyl-tRNA(Gln) amidotransferase, B subunit (RefSeq))
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00133.hmm
# target sequence database:        /tmp/gapView.1659.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00133  [M=481]
Accession:   TIGR00133
Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
     4e-164  532.6   0.0   4.7e-164  532.4   0.0    1.0  1  lcl|FitnessBrowser__Dino:3607799  Dshi_1207 glutamyl-tRNA(Gln) ami


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Dino:3607799  Dshi_1207 glutamyl-tRNA(Gln) amidotransferase, B subunit (RefSeq)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  532.4   0.0  4.7e-164  4.7e-164       2     480 ..      19     502 ..      18     503 .. 0.95

  Alignments for each domain:
  == domain 1  score: 532.4 bits;  conditional E-value: 4.7e-164
                         TIGR00133   2 ieyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalnskivsevsv 78 
                                       +++elviGlEvH q+ t++KlF+ +s+ +   +pN+nv  v+ g+PG lPv+N+e v++A+  +l l++ i+  +s 
  lcl|FitnessBrowser__Dino:3607799  19 EDWELVIGLEVHAQVATNAKLFSGASTLFGA-EPNSNVAFVDAGMPGMLPVINEECVAQAVRTGLGLKAAIN-LTSA 93 
                                       5789*************************99.**************************************76.59** PP

                         TIGR00133  79 FdRKhYfYpDlPkgyqitqqdlPiaedGkleieleeke.keigierlhlEeDtgksqykesdkdkqslvDfNRsgvP 154
                                       FdRK+YfYpDlP+gyqi+q + Pi+ +G++ +el ++  + ++ier+hlE+D+gks +  +  +++s+vD+NR+gv 
  lcl|FitnessBrowser__Dino:3607799  94 FDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVELGDGTaRVVRIERIHLEQDAGKSIHDMD--PHMSFVDLNRTGVA 168
                                       *********************************977654999****************976..78************ PP

                         TIGR00133 155 LlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsirlkGq..........ekegtrvEiKNl 221
                                       L+EiV++Pd++ ++ea+a+++klrqi+ryl+  dg++++G++R+DvNvs+   G+          ++ gtr EiKN+
  lcl|FitnessBrowser__Dino:3607799 169 LMEIVSRPDIRGPEEAAAYVSKLRQIMRYLGTCDGNMQNGNLRADVNVSVCRPGDyekyqasqdfSHLGTRCEIKNM 245
                                       **************************************************87765222222222245689******* PP

                         TIGR00133 222 nslksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDYRYfpePdlppieideevvkekvee 298
                                       nsl+ i++aieyE  Rq++++++g +v+qetr +d  k  t s+R+Kee++DYRYfp+Pdl p+ei++ +v++ +++
  lcl|FitnessBrowser__Dino:3607799 246 NSLRFIQQAIEYEARRQIAIIEDGGSVTQETRLYDPDKGETRSMRSKEEAHDYRYFPDPDLLPLEIEQAWVDD-IAA 321
                                       ************************************************************************9.*** PP

                         TIGR00133 299 klpelPeakrirlkkeyglseedakvlvsdlelldafeevvklikepklavnWileellgeLnkkkislaeallkpe 375
                                        lpelP+ak++r+ +e+gls++da vl++d++ + +fe v+  +++ kla+nW+++el g+L+k++ +++e+ ++p 
  lcl|FitnessBrowser__Dino:3607799 322 ALPELPDAKKARFITEFGLSDYDASVLTADTTNAAYFEAVAGEAGDGKLAANWVINELFGRLKKEDHDITESPVSPA 398
                                       ***************************************************************************** PP

                         TIGR00133 376 elaeliklikegkisqksakelleellenkkdpkklieklgliqisdekelvkiveevikenpkevekyksgkekal 452
                                       +la +iklik++ is+k+ak+l+e + ++++dp +++e++g+ q++d+ ++ ++v+e+i+ np +v k +++  k+ 
  lcl|FitnessBrowser__Dino:3607799 399 QLAGIIKLIKADAISGKIAKDLFEIVYTEGGDPAEIVEARGMKQVTDTGAIEAAVDEIIAANPAQVAKAQEN-PKLA 474
                                       ******************************************************************998765.6788 PP

                         TIGR00133 453 kflvGqvmkktkgradpkevekllkell 480
                                       +++vGqvmk t g+a+pk v++++  +l
  lcl|FitnessBrowser__Dino:3607799 475 GWFVGQVMKATGGKANPKAVNQIVAAKL 502
                                       9**********************98877 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (481 nodes)
Target sequences:                          1  (505 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 10.62
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis