Align phosphoglycerate dehydrogenase; EC 1.1.1.95 (characterized)
to candidate 3609936 Dshi_3318 D-3-phosphoglycerate dehydrogenase (RefSeq)
Query= CharProtDB::CH_004924 (525 letters) >FitnessBrowser__Dino:3609936 Length = 531 Score = 413 bits (1062), Expect = e-120 Identities = 218/513 (42%), Positives = 320/513 (62%), Gaps = 9/513 (1%) Query: 3 RVLVSDKMSNDGLQPL----IESDFIEIVQKNVADAEDELHTFDALLVRSATKVTEDLFN 58 +VLVSDK+S +Q IE DF + K+ + + +D L +RSATKVTE L Sbjct: 4 KVLVSDKLSETAVQIFRDRGIEVDFEPSLGKDKDKLAEVIGKYDGLAIRSATKVTEKLLA 63 Query: 59 KMTSLKIVGRAGVGVDNIDIDEATKHGVIVINAPNGNTISTAEHTFAMISSLMRHIPQAN 118 +LK+VGRAG+G DN+D A+K GVIV+N P GN I+TAEH AM+ ++ R IP+A+ Sbjct: 64 HADNLKVVGRAGIGTDNVDKVAASKKGVIVMNTPFGNMITTAEHAIAMMFAVARQIPEAS 123 Query: 119 ISVKSREWNRTAYVGSELYGKTLGIVGLGRIGSEIAQRRGAFGMTVHVFDPFLTEERAKK 178 S + +W ++ ++G EL KTLG++G G IG + +R M V +DPFL+EERA K Sbjct: 124 ASTHAGKWEKSKFMGVELTAKTLGVIGAGNIGGIVCERALGLKMKVIAYDPFLSEERADK 183 Query: 179 IGVNSRTFEEVLESADIITVHTPLTKETKGLLNKETIAKTKKGVRLINCARGGIIDEAAL 238 +GV + +L AD IT+H PLT +T+ +L++E + KTKKGVR+INCARGG++DE AL Sbjct: 184 LGVEKVDLDTLLARADFITLHVPLTDQTRNILSREALEKTKKGVRIINCARGGLVDEQAL 243 Query: 239 LEALENGHVAGAALDVFEVEPPVDNKLVDHPLVIATPHLGASTKEAQLNVAAQVSEEVLQ 298 + L++GHVAGAA DVF EP +N L P V+ TPHLGAST EAQ NVA QV+E++ Sbjct: 244 ADLLKSGHVAGAAFDVFAEEPATENPLFGLPNVVCTPHLGASTTEAQENVALQVAEQMSN 303 Query: 299 FAKGLPVMSAINLPAMTKDEFAKIKPYHQIAGKIGSLVSQCMKEPVQDVAIQYEGTIAKL 358 + V +A+N+P++T +E + P+ ++AG +G+ V Q EP+ + I Y GT+AK+ Sbjct: 304 YLLSGAVENALNMPSVTAEEAKTMGPWVKLAGHLGTFVGQLTDEPIDSIEILYNGTVAKM 363 Query: 359 ETSFITKALLSGFLKPRVDSTVNEVNAGGVAKERGISFSEKISSSESGYDNCISVKVTGD 418 + A+++G ++ + VN V+A +AKERGI S +D + + V D Sbjct: 364 NLEALNCAVIAGIMQ-ATNPDVNMVSAPVIAKERGIGLSTTTQDKSGVFDGYVKLTVKTD 422 Query: 419 RSTFTVTATYIPHFGERIVEINGFNIDFYPTGHLVYIQHQDTTGVIGRVGRILGDNDINI 478 + T ++ T R ++I G NID H++Y ++DT G+IG +G ILG+N +NI Sbjct: 423 QRTRSIAGTVFSDGKPRFIQIKGINIDAEIGEHMLYTTNKDTPGMIGTLGGILGENQVNI 482 Query: 479 ATMQVGRKEKGGEAIMMLSFD----RHLEDKIV 507 A +GR E G+AI ML D R ++DK++ Sbjct: 483 ANFTLGRNETNGDAIAMLYLDAPLPRDVQDKLI 515 Lambda K H 0.317 0.134 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 639 Number of extensions: 26 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 1 Length of query: 525 Length of database: 531 Length adjustment: 35 Effective length of query: 490 Effective length of database: 496 Effective search space: 243040 Effective search space used: 243040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
Align candidate 3609936 Dshi_3318 (D-3-phosphoglycerate dehydrogenase (RefSeq))
to HMM TIGR01327 (serA: phosphoglycerate dehydrogenase (EC 1.1.1.95))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01327.hmm # target sequence database: /tmp/gapView.24824.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01327 [M=525] Accession: TIGR01327 Description: PGDH: phosphoglycerate dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.5e-203 663.2 3.8 1.7e-203 663.1 3.8 1.0 1 lcl|FitnessBrowser__Dino:3609936 Dshi_3318 D-3-phosphoglycerate d Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Dino:3609936 Dshi_3318 D-3-phosphoglycerate dehydrogenase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 663.1 3.8 1.7e-203 1.7e-203 1 520 [. 4 523 .. 4 528 .. 0.98 Alignments for each domain: == domain 1 score: 663.1 bits; conditional E-value: 1.7e-203 TIGR01327 1 kvlvadklseegiellkekelevdvktgl..skeellekikdydalivRSatkvteelleaaekLkvigRaGvGvDN 75 kvlv+dklse+++++++++++evd + l +k++l e+i +yd+l +RSatkvte+ll++a++Lkv+gRaG+G DN lcl|FitnessBrowser__Dino:3609936 4 KVLVSDKLSETAVQIFRDRGIEVDFEPSLgkDKDKLAEVIGKYDGLAIRSATKVTEKLLAHADNLKVVGRAGIGTDN 80 7***********************9987622677899**************************************** PP TIGR01327 76 idieaatkkGilvvNaPegntisaaElalalllalaRkipqadksvkekkWerkkflGtElygktlGviGlGriGse 152 +d aa+kkG++v+N+P gn i++aE+a+a+++a+aR+ip+a +s++++kWe++kf+G+El++ktlGviG+G+iG + lcl|FitnessBrowser__Dino:3609936 81 VDKVAASKKGVIVMNTPFGNMITTAEHAIAMMFAVARQIPEASASTHAGKWEKSKFMGVELTAKTLGVIGAGNIGGI 157 ***************************************************************************** PP TIGR01327 153 vakrakalgmkvlayDPyiseekaeklgvellkdldellaeaDvitvHvPltketkgligkeelakmKkgviivNca 229 v +ra +l+mkv+ayDP++see+a+klgve + dld+lla+aD+it+HvPlt++t++++++e+l+k+Kkgv+i+Nca lcl|FitnessBrowser__Dino:3609936 158 VCERALGLKMKVIAYDPFLSEERADKLGVE-KVDLDTLLARADFITLHVPLTDQTRNILSREALEKTKKGVRIINCA 233 ******************************.556******************************************* PP TIGR01327 230 RGGiidEkALvealeegkvraaalDvfekEPptdnklleldnvvvtpHlgAsteEaqenvavevaeevlealkgevv 306 RGG++dE+AL+++l++g+v++aa+Dvf++EP+t+n+l+ l+nvv tpHlgAst+Eaqenva++vae+++++l +v lcl|FitnessBrowser__Dino:3609936 234 RGGLVDEQALADLLKSGHVAGAAFDVFAEEPATENPLFGLPNVVCTPHLGASTTEAQENVALQVAEQMSNYLLSGAV 310 ***************************************************************************** PP TIGR01327 307 esavNlpaldaeeleklkpyldlaeklGklasqllkeavkkvevtleGelaeeeselltsallkgllkevleeevnl 383 e+a+N+p+++aee++++ p+++la +lG++++ql++e ++++e+ ++G++a+++ e+l+ a+++g++++ ++ +vn+ lcl|FitnessBrowser__Dino:3609936 311 ENALNMPSVTAEEAKTMGPWVKLAGHLGTFVGQLTDEPIDSIEILYNGTVAKMNLEALNCAVIAGIMQA-TNPDVNM 386 ******************************************************************975.678**** PP TIGR01327 384 vnAkavakergitveeskeeesedyknllevkveadkgevsvagtvleekepriveidgfevdlepegilliiknkD 460 v+A+++akergi ++ +++++s ++ +++++v++d+ ++s+agtv+++ +pr ++i+g+++d e +++l+++nkD lcl|FitnessBrowser__Dino:3609936 387 VSAPVIAKERGIGLSTTTQDKSGVFDGYVKLTVKTDQRTRSIAGTVFSDGKPRFIQIKGINIDAEIGEHMLYTTNKD 463 ***************************************************************************** PP TIGR01327 461 kpGvigkvgsllgeagiNiasmqlgrkekggealmllslDeevseevleeikevpeiksv 520 +pG+ig +g +lge+++Nia+++lgr+e++g+a+++l lD +++ +v +++ + ++v lcl|FitnessBrowser__Dino:3609936 464 TPGMIGTLGGILGENQVNIANFTLGRNETNGDAIAMLYLDAPLPRDVQDKLIATGLFQQV 523 ************************************************999877666665 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (525 nodes) Target sequences: 1 (531 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.02s 00:00:00.04 Elapsed: 00:00:00.03 # Mc/sec: 8.39 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory