GapMind for Amino acid biosynthesis

 

Protein N515DRAFT_2409 in Dyella japonica UNC79MFTsu3.2

Annotation: N515DRAFT_2409 dihydroxy-acid dehydratase

Length: 574 amino acids

Source: Dyella79 in FitnessBrowser

Candidate for 3 steps in Amino acid biosynthesis

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-isoleucine biosynthesis ilvD lo Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (uncharacterized) 40% 99% 359.4 L-arabonate dehydratase (EC 4.2.1.25) 73% 837.8
L-leucine biosynthesis ilvD lo Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (uncharacterized) 40% 99% 359.4 L-arabonate dehydratase (EC 4.2.1.25) 73% 837.8
L-valine biosynthesis ilvD lo Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (uncharacterized) 40% 99% 359.4 L-arabonate dehydratase (EC 4.2.1.25) 73% 837.8

Sequence Analysis Tools

View N515DRAFT_2409 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MGSPKKPLRRSQEWFGREGKQGFYYRSWLKGLGLPNDMFDGRPVIGICNTWSELTPCNSS
FRELAEHVKRGVYEAGGFPLEFPVMSLGETQMRPTAMLFRNLASMDVEESIRANPIDGVV
LLMGCDKTTPALMMGAASVNLPTIGLSGGPSLSGNWRGQPIGSGTGVIRMSEMVRAGELA
QEEFVEAEACMQRSKGSCMTMGTASTMASMVEALGLSLPENAAIPAVDSRRFRLAHLSGR
RIVEMVHEDLRMSKILTRAAFENAIRANAAIGGSTNAVIHLLALAGRLGVELALDDWDRL
GSHLPCLVNLKPSGDYLMEDFYYAGGLPAVLREIAPHLHLGALTANGRTLGDNIATAPCW
NRDVIRPIEEPLKREAGIAVLRGNLAPDGAVIKPSAASAHLLQHRGRAVVFENIDDFKAR
IDDEALDIDETCVMVLKNCGPRGYPGMAEVGNMPLPPKLLRRGITDIVRISDARMSGTAY
GTVVLHASPEAAAGGNLALVRDGDFIELDVPGRRLHLDLADDELARRRAAWKAPPPPERG
WAKLYVDHVQQAHLGADLDILVGGSGSDVAGDSH

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory