Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate N515DRAFT_3260 N515DRAFT_3260 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase
Query= BRENDA::P29976 (525 letters) >FitnessBrowser__Dyella79:N515DRAFT_3260 Length = 472 Score = 411 bits (1057), Expect = e-119 Identities = 203/443 (45%), Positives = 290/443 (65%), Gaps = 5/443 (1%) Query: 75 WTPESWKLKKALQLPDYPNANELESVLKTIEAFPPIVFAGEARNLEERLADAAVGKAFLL 134 WTP SW+L++A Q P Y + +LE+V + I PP+V +GE + L+ LADA G+ FLL Sbjct: 14 WTPASWRLREAQQQPCYDDRAQLENVARRIGKLPPLVTSGEIQELKRALADAQEGRRFLL 73 Query: 135 QGGDCAESFKEFNATNIRDTFRVLLQMSIVLTFGGQVPVIKVGRMAGQFAKPRSDAFEEK 194 GGDCAESF + +A I + +VL QMS++L G + PVI+VGR AGQ+AKPRS E Sbjct: 74 HGGDCAESFGDCDADTIVNRLKVLFQMSLILGHGLKQPVIRVGRFAGQYAKPRSSDRETV 133 Query: 195 DGVKLPSYKGDNINGDTFDEKSRIPDPNRMIRAYTQSAATLNLLRAFATGGYAAIQRVTQ 254 G LPS++GD +N FD +R PDP RM +AY SA TLN RA GG+ ++ Sbjct: 134 GGETLPSFRGDIVNAAAFDAAARRPDPERMFQAYAFSALTLNFTRALFEGGFGDLRHPEY 193 Query: 255 WNLDFVEQSEQADRYQELANRVDEALGFMSACG--LGTDHPLMTTTDFYTSHECLLLPYE 312 W LD+++QS +A Y+ + + F+ G +GT+ + FYTSHE LLLP+E Sbjct: 194 WQLDWIDQSPRAIEYRREVEEIANSWRFLHTLGGQVGTER---VSAGFYTSHEALLLPFE 250 Query: 313 QSLTRLDSTSGLYYDCSAHMVWCGERTRQLDGAHVEFLRGIANPLGIKVSNKMDPFELVK 372 +++TR +++ S H+ W G RT +DGAH+E+ RG+ NP+ +KV P +L++ Sbjct: 251 EAVTRRVPHRRGWFNLSTHLPWIGMRTSDIDGAHIEYFRGLQNPVAMKVGPSTRPADLLR 310 Query: 373 LVEILNPNNKPGRITVIVRMGAENMRVKLPHLIRAVRRSGQIVTWVCDPMHGNTIKAPCG 432 L+E+LNP+ +PGR+T+I RMG +++ LP L+ AVRR+G+ V WV DPMHGNT P G Sbjct: 311 LIEVLNPHEEPGRMTLITRMGCKHIAEALPPLLAAVRRAGRHVLWVVDPMHGNTETTPTG 370 Query: 433 LKTRAFDSILAEVRAFLDVHEQEGSHAGGIHLEMTGQNVTECIGGSRTVTYDDLSSRYHT 492 +KTR FD+I +E+ A +H EG+ GG+HLEMTG++VTEC+GG+R++ DLS Y T Sbjct: 371 IKTRHFDNIRSELEAAFQIHGAEGTRLGGVHLEMTGEDVTECLGGARSLQEQDLSRAYKT 430 Query: 493 HCDPRLNASQSLELAFIVAERLR 515 DPRLN Q++ELA ++ + R Sbjct: 431 TVDPRLNYEQAMELAMLITKVAR 453 Lambda K H 0.318 0.132 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 594 Number of extensions: 23 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 525 Length of database: 472 Length adjustment: 34 Effective length of query: 491 Effective length of database: 438 Effective search space: 215058 Effective search space used: 215058 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate N515DRAFT_3260 N515DRAFT_3260 (3-deoxy-D-arabinoheptulosonate-7-phosphate synthase)
to HMM TIGR01358 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01358.hmm # target sequence database: /tmp/gapView.19732.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01358 [M=443] Accession: TIGR01358 Description: DAHP_synth_II: 3-deoxy-7-phosphoheptulonate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.2e-194 630.7 0.0 5.9e-194 630.5 0.0 1.0 1 lcl|FitnessBrowser__Dyella79:N515DRAFT_3260 N515DRAFT_3260 3-deoxy-D-arabino Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Dyella79:N515DRAFT_3260 N515DRAFT_3260 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 630.5 0.0 5.9e-194 5.9e-194 1 441 [. 14 454 .. 14 456 .. 0.99 Alignments for each domain: == domain 1 score: 630.5 bits; conditional E-value: 5.9e-194 TIGR01358 1 wsleswrskpaaqlPeyPdaealeavldtleslPPlvlageilklkeklaevakGeafllqgGdca 66 w+++swr ++a+q+P y d ++le+v + +lPPlv++gei++lk+ la++++G++fll gGdca lcl|FitnessBrowser__Dyella79:N515DRAFT_3260 14 WTPASWRLREAQQQPCYDDRAQLENVARRIGKLPPLVTSGEIQELKRALADAQEGRRFLLHGGDCA 79 89**************************************************************** PP TIGR01358 67 esfkeveadnirdklrvllqmavvltygaslPvvkvgriaGqyakPrsepieakdgvtlpsyrGdv 132 esf +++ad+i ++l+vl+qm+++l +g + Pv++vgr+aGqyakPrs++ e+ +g tlps+rGd+ lcl|FitnessBrowser__Dyella79:N515DRAFT_3260 80 ESFGDCDADTIVNRLKVLFQMSLILGHGLKQPVIRVGRFAGQYAKPRSSDRETVGGETLPSFRGDI 145 ****************************************************************** PP TIGR01358 133 ingaafdeaarvpdperlvrayaksaatlnllraltsgGyadlkkvheWnlefvkkspagaryekl 198 +n+aafd+aar+pdper+ +aya sa tln+ ral +gG+ dl++ ++W+l+++ +sp + +y++ lcl|FitnessBrowser__Dyella79:N515DRAFT_3260 146 VNAAAFDAAARRPDPERMFQAYAFSALTLNFTRALFEGGFGDLRHPEYWQLDWIDQSPRAIEYRRE 211 ****************************************************************** PP TIGR01358 199 aeeidealrfmsavgvaeaealkrvelytsheallldyeealtrvdskegqlfdlsahllWiGert 264 +eei ++ rf++++g + ++ + +ytshealll++eea+tr +++++++f+ls+hl WiG+rt lcl|FitnessBrowser__Dyella79:N515DRAFT_3260 212 VEEIANSWRFLHTLGGQVGTERVSAGFYTSHEALLLPFEEAVTRRVPHRRGWFNLSTHLPWIGMRT 277 *****************99999999***************************************** PP TIGR01358 265 rqldgahveflrgvknPigikvgpsmeadellklievldPenePGrltlisrlGaekiaeklPell 330 +dgah+e+ rg++nP+++kvgps+++ +ll+lievl+P++ePGr+tli+r+G ++iae lP+ll lcl|FitnessBrowser__Dyella79:N515DRAFT_3260 278 SDIDGAHIEYFRGLQNPVAMKVGPSTRPADLLRLIEVLNPHEEPGRMTLITRMGCKHIAEALPPLL 343 ****************************************************************** PP TIGR01358 331 eavkaaGravvWvtdpmhGntleaasGyktrrfddilsevkeffevhkaeGthpGGvhleltGedv 396 av++aGr+v+Wv dpmhGnt+++++G ktr+fd+i+se+++ f++h aeGt++GGvhle+tGedv lcl|FitnessBrowser__Dyella79:N515DRAFT_3260 344 AAVRRAGRHVLWVVDPMHGNTETTPTGIKTRHFDNIRSELEAAFQIHGAEGTRLGGVHLEMTGEDV 409 ****************************************************************** PP TIGR01358 397 teclGGareitetdlasryetacdPrlnaeqslelaflvaeklre 441 teclGGar+++e+dl+++y+t++dPrln+eq++ela+l+++ r+ lcl|FitnessBrowser__Dyella79:N515DRAFT_3260 410 TECLGGARSLQEQDLSRAYKTTVDPRLNYEQAMELAMLITKVARA 454 ***************************************998875 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (443 nodes) Target sequences: 1 (472 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01 # Mc/sec: 12.57 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory