Alignments for a candidate for hisH in Dyella japonica UNC79MFTsu3.2

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisH in Dyella japonica UNC79MFTsu3.2

Align IGP synthase glutamine amidotransferase subunit; EC 2.4.2.- (characterized)
to candidate N515DRAFT_2934 N515DRAFT_2934 glutamine amidotransferase

Query= CharProtDB::CH_024511
         (196 letters)



>FitnessBrowser__Dyella79:N515DRAFT_2934
          Length = 196

 Score =  177 bits (450), Expect = 8e-50
 Identities = 91/197 (46%), Positives = 129/197 (65%), Gaps = 2/197 (1%)

Query: 1   MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFLPGVGTAQAAMDQVRER 60
           M VV++D G  N+ SV+ A+ R G +  ++ D + +  AD++ LPGVG A   M ++RE 
Sbjct: 1   MAVVLVDAGGTNIGSVRYALQRLGVDAALTSDANAIRQADRVILPGVGAAGPGMARLREL 60

Query: 61  ELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDEDVPKMTDF-GLPLPHMGWN 119
            L +LI++ TQPVLG+CLGMQLL  RSEE    + L +I     +  +  GL +PHMGWN
Sbjct: 61  GLVELIRSLTQPVLGVCLGMQLLFERSEEG-ATECLAVIPGAASRFAEAPGLRVPHMGWN 119

Query: 120 RVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYGEPFTAAVQKDNFYGVQFHP 179
           R+  +  + L  G+   A+ YFVHSYA+P N  T+A  ++G  F+A V + NF+G+QFHP
Sbjct: 120 RLRVERAHPLVAGLGTDAWAYFVHSYAVPANDSTLATADFGGAFSAVVARGNFHGMQFHP 179

Query: 180 ERSGAAGAKLLKNFLEM 196
           ERS   GA+LLKNFLE+
Sbjct: 180 ERSAGVGARLLKNFLEL 196


Lambda     K      H
   0.322    0.140    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 129
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 196
Length of database: 196
Length adjustment: 20
Effective length of query: 176
Effective length of database: 176
Effective search space:    30976
Effective search space used:    30976
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 45 (21.9 bits)

Align candidate N515DRAFT_2934 N515DRAFT_2934 (glutamine amidotransferase)
to HMM TIGR01855 (hisH: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (EC 2.4.2.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01855.hmm
# target sequence database:        /tmp/gapView.6816.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01855  [M=198]
Accession:   TIGR01855
Description: IMP_synth_hisH: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                    Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                    -----------
    1.1e-68  217.0   0.0    1.2e-68  216.8   0.0    1.0  1  lcl|FitnessBrowser__Dyella79:N515DRAFT_2934  N515DRAFT_2934 glutamine amidotr


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Dyella79:N515DRAFT_2934  N515DRAFT_2934 glutamine amidotransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  216.8   0.0   1.2e-68   1.2e-68       1     197 [.       3     195 ..       3     196 .] 0.97

  Alignments for each domain:
  == domain 1  score: 216.8 bits;  conditional E-value: 1.2e-68
                                    TIGR01855   1 ivvidygvgNlksvkkalervgaesevvkdskelekadklvlPGVGafkeamkklrelelellaek 66 
                                                  +v++d+g  N+ sv+ al+r+g+ + +++d++++++ad+++lPGVGa+  +m++lrel+   l+e 
  lcl|FitnessBrowser__Dyella79:N515DRAFT_2934   3 VVLVDAGGTNIGSVRYALQRLGVDAALTSDANAIRQADRVILPGVGAAGPGMARLRELG---LVEL 65 
                                                  799*******************************************************9...4477 PP

                                    TIGR01855  67 vvkkkkpvlgiClGmQllfekseEgkevkglglikgkvkkleaek..kvPhiGWnevevvkesell 130
                                                  +++ ++pvlg+ClGmQllfe+seEg ++++l++i+g+ +++ ++   +vPh+GWn++ v ++++l+
  lcl|FitnessBrowser__Dyella79:N515DRAFT_2934  66 IRSLTQPVLGVCLGMQLLFERSEEG-ATECLAVIPGAASRFAEAPglRVPHMGWNRLRVERAHPLV 130
                                                  8999********************6.68**************99899******************* PP

                                    TIGR01855 131 kgleeearvYfvHsYaveleeeeavlakadygekfvaavekdnivgvQFHPEkSgktGlkllknfl 196
                                                  +gl ++a +YfvHsYav+ ++   +la+ad+g  f a+v+++n+ g+QFHPE+S+ +G++llknfl
  lcl|FitnessBrowser__Dyella79:N515DRAFT_2934 131 AGLGTDAWAYFVHSYAVPAND--STLATADFGGAFSAVVARGNFHGMQFHPERSAGVGARLLKNFL 194
                                                  ******************997..8*****************************************9 PP

                                    TIGR01855 197 e 197
                                                  e
  lcl|FitnessBrowser__Dyella79:N515DRAFT_2934 195 E 195
                                                  8 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (198 nodes)
Target sequences:                          1  (196 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 4.99
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis