Alignments for a candidate for prs in Dyella japonica UNC79MFTsu3.2

GapMind for Amino acid biosynthesis

Alignments for a candidate for prs in Dyella japonica UNC79MFTsu3.2

Align Ribose-phosphate pyrophosphokinase; RPPK; EC 2.7.6.1; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase (uncharacterized)
to candidate N515DRAFT_2463 N515DRAFT_2463 ribose-phosphate pyrophosphokinase

Query= curated2:Q8TUT6
         (291 letters)



>FitnessBrowser__Dyella79:N515DRAFT_2463
          Length = 293

 Score =  127 bits (319), Expect = 3e-34
 Identities = 90/290 (31%), Positives = 143/290 (49%), Gaps = 8/290 (2%)

Query: 2   IVLATSEGVKLGRRLAEELDAELAPVEEDRFPDGEQIVRV-PPELDGTVVVVHSMSPPQD 60
           +V A     +L   LA         +E  RFPDGE +VRV  P +    ++V ++  P D
Sbjct: 4   VVYAMPGNQELADALAHRRRMARLALELHRFPDGEGLVRVEEPPVGAEAIIVCTLDRP-D 62

Query: 61  ENLVKAIITLDAARENGAEEVIAIVPYMAYSRQDRRFEPGEPVSFRAVARAVSANADALI 120
           E  +  ++  D  RE GA  V  + PY+AY RQD RF PGE VS     R ++ + D  +
Sbjct: 63  EKTLPLLMAADTLRELGAGSVGLVAPYLAYLRQDARFRPGEAVSSGIFGRLLAEHFDWQV 122

Query: 121 TVD--LHEPGTLKYFDVP-AENVSAAEELGKYLAERFEGEDLVVIGPDEGARELAREVAS 177
           T+D  LH   +L    +P    +SAA  L  +L ER E    ++IGPDE +      VA+
Sbjct: 123 TIDPHLHRLRSLTEAGMPQGRAISAAPALAGWLRERVELP--LLIGPDEESAPWVESVAA 180

Query: 178 ICGVEYDHLEKKRLSGDEVEIHPKEL-DVEGRTVVLVDDMIDTGGTMVEAARALRDQGAG 236
           +         K+R    +V++   +L +  G   VL+DD+I +G T++++   LR  G  
Sbjct: 181 LMSAPAVVATKRRFGDRKVQVQLPDLSNWPGHNPVLLDDIISSGHTLLKSIEGLRALGMP 240

Query: 237 TLYAACTHALLTRNAATRLLASGFEDIIATDTVPNPFEKVSVAPPVAEAV 286
             +    H L   NA  RL  +G   ++ T++VP    ++ ++  +A A+
Sbjct: 241 APWCVAVHGLFADNALERLRVAGVAGVVTTNSVPGETARIDLSELLAAAL 290


Lambda     K      H
   0.314    0.134    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 178
Number of extensions: 8
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 291
Length of database: 293
Length adjustment: 26
Effective length of query: 265
Effective length of database: 267
Effective search space:    70755
Effective search space used:    70755
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 48 (23.1 bits)

Align candidate N515DRAFT_2463 N515DRAFT_2463 (ribose-phosphate pyrophosphokinase)
to HMM TIGR01251 (prs: ribose-phosphate diphosphokinase (EC 2.7.6.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01251.hmm
# target sequence database:        /tmp/gapView.31276.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01251  [M=309]
Accession:   TIGR01251
Description: ribP_PPkin: ribose-phosphate diphosphokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                    Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                    -----------
    4.7e-52  162.8   0.0    5.3e-52  162.6   0.0    1.0  1  lcl|FitnessBrowser__Dyella79:N515DRAFT_2463  N515DRAFT_2463 ribose-phosphate 


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Dyella79:N515DRAFT_2463  N515DRAFT_2463 ribose-phosphate pyrophosphokinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  162.6   0.0   5.3e-52   5.3e-52       5     295 ..       7     290 ..       3     293 .] 0.93

  Alignments for each domain:
  == domain 1  score: 162.6 bits;  conditional E-value: 5.3e-52
                                    TIGR01251   5 sgssskelaekvaknlglelgdvevkkFadgElyvrieesvrgkdvfiivqstsapvndalmelll 70 
                                                  + + ++ela+++a++  +  +  e ++F+dgE  vr+ee   g + +i+   t    +++ + ll+
  lcl|FitnessBrowser__Dyella79:N515DRAFT_2463   7 AMPGNQELADALAHRRRMARLALELHRFPDGEGLVRVEEPPVGAEAIIV--CTLDRPDEKTLPLLM 70 
                                                  56789**************************************999999..677789********* PP

                                    TIGR01251  71 lidalkrasaksvtaviPyygYaRqdkkaksrepisaklvaklleeaGadrvltvdlHseqiqgfF 136
                                                  ++d+l++ +a+sv +v+Py++Y Rqd +++++e++s+ ++++ll+e   d  +t+d H + +++  
  lcl|FitnessBrowser__Dyella79:N515DRAFT_2463  71 AADTLRELGAGSVGLVAPYLAYLRQDARFRPGEAVSSGIFGRLLAEH-FDWQVTIDPHLHRLRSLT 135
                                                  ***********************************************.************999999 PP

                                    TIGR01251 137 d...vpvenlsaspklieelkkkelknlvvvsPDkGaverakkvakklglelaiieKeRdskenev 199
                                                  +    + + +sa+p l+ +l+++ ++ ++++ PD+ ++ ++++va+ + ++ ++  K+R  ++ +v
  lcl|FitnessBrowser__Dyella79:N515DRAFT_2463 136 EagmPQGRAISAAPALAGWLRER-VELPLLIGPDEESAPWVESVAALMSAPAVVATKRRFGDR-KV 199
                                                  843124477999*********97.9999********************************555.77 PP

                                    TIGR01251 200 evt.nllgdvegkdvvivDDiisTggTlvkaaelLkekGAkkvivaathgvfsgdAlerlaeagve 264
                                                  +v+ + l +  g + v++DDiis+g+Tl+k++e L+  G     ++a+hg+f+++Alerl+ agv 
  lcl|FitnessBrowser__Dyella79:N515DRAFT_2463 200 QVQlPDLSNWPGHNPVLLDDIISSGHTLLKSIEGLRALGMPAPWCVAVHGLFADNALERLRVAGVA 265
                                                  7762556799******************************************************** PP

                                    TIGR01251 265 evivtntilveekklpkvseisvapliaeai 295
                                                   v+ tn+++       +  +i++++l+a a+
  lcl|FitnessBrowser__Dyella79:N515DRAFT_2463 266 GVVTTNSVPG------ETARIDLSELLAAAL 290
                                                  **********......889999999999887 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (309 nodes)
Target sequences:                          1  (293 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.31
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis