Alignments for a candidate for asnB in Herbaspirillum seropedicae SmR1

GapMind for Amino acid biosynthesis

Alignments for a candidate for asnB in Herbaspirillum seropedicae SmR1

Align candidate HSERO_RS21075 HSERO_RS21075 (asparagine synthase)
to HMM TIGR01536 (asnB: asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01536.hmm
# target sequence database:        /tmp/gapView.23568.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01536  [M=517]
Accession:   TIGR01536
Description: asn_synth_AEB: asparagine synthase (glutamine-hydrolyzing)
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                  Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                  -----------
   9.4e-127  410.0   0.0   1.3e-126  409.6   0.0    1.2  1  lcl|FitnessBrowser__HerbieS:HSERO_RS21075  HSERO_RS21075 asparagine synthas


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__HerbieS:HSERO_RS21075  HSERO_RS21075 asparagine synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  409.6   0.0  1.3e-126  1.3e-126       1     517 []       2     562 ..       2     562 .. 0.89

  Alignments for each domain:
  == domain 1  score: 409.6 bits;  conditional E-value: 1.3e-126
                                  TIGR01536   1 CgiagivdlkakakeeeeaikemletlahRGPDaegvwkde.keenailghrRLaiidlseg.aQPls 66 
                                                Cgiag ++++ +++++   +++ l+ ++ RGP+++ ++++      a+lg+ RL++idlse+  QP++
  lcl|FitnessBrowser__HerbieS:HSERO_RS21075   2 CGIAGSFWKNSDLQSN-IRMERALQIMRERGPNHQDYHRHDtAGGSALLGQTRLSVIDLSEQaSQPMY 68 
                                                ***********99996.56777799***********9999889999**************999***** PP

                                  TIGR01536  67 nek.evvivfnGEIYNheeLreeleekGyeFetksDtEViLaayeewgeelverLeGmFAfalwdekk 133
                                                + + ++ ++fnGEIYN+ eLreel ++G +F+t+sDtEV+Laa+e+wge+++ rL+GmFAfa++d + 
  lcl|FitnessBrowser__HerbieS:HSERO_RS21075  69 SPDgRYGLIFNGEIYNYIELREELARAGRNFHTQSDTEVLLAAWEAWGEAALRRLTGMFAFAVFDFQL 136
                                                ******************************************************************** PP

                                  TIGR01536 134 gelflaRDrlGikPLYyaseqgkllfaSEiKallalkeikaeldkealaelltlq.lvptektlfkev 200
                                                ++  l+RD +GikPL+yas+++ ++faS  +a+ al    ++l+ + + ++l +  ++  ++t+f e 
  lcl|FitnessBrowser__HerbieS:HSERO_RS21075 137 RTCTLVRDAFGIKPLFYASTGEGFYFASDTRAMRALAPCSPALNWQRAYDYLVHGeYDFGNETFFSEF 204
                                                *************************************99*****99999999999999********** PP

                                  TIGR01536 201 kelepakal....dgeeklee..ywevekee.vkeseeelveelrelledavkkrlvadvpvgvllSG 261
                                                  l+p++      d+++++    +w+ +  +  ++s+ee+  +lrell ++vk +l++dvp+g+ lSG
  lcl|FitnessBrowser__HerbieS:HSERO_RS21075 205 SSLSPGHMIevplDQARTMLPrcWWKPSIIKdSTSSFEEASMQLRELLLESVKLHLRSDVPLGAALSG 272
                                                ********987764444444479****998878999******************************** PP

                                  TIGR01536 262 GlDSslvaaiakkeak.sevktFsigfedskdldeskaarkvadelgtehkevliseeevlkeleevi 328
                                                G+DSs++++ +++  +  +++tFs     +++++e+++   v +++g   ++v++++ee++++le +i
  lcl|FitnessBrowser__HerbieS:HSERO_RS21075 273 GIDSSVIVCAMRQIEPdLPIHTFSFVAS-ESAVSEEHWVDLVNKHVGAIAHKVHVTPEELAHDLESLI 339
                                                ************9998899******999.99************************************* PP

                                  TIGR01536 329 laleeptairasiplyllsklarekgvkVvLsGeGaDElfgGYeyfreakaeeale............ 384
                                                 ++ ep+  ++++ +y ++kla+++gv+V L+G+GaDE++gGY+ +  + +   l             
  lcl|FitnessBrowser__HerbieS:HSERO_RS21075 340 DTQGEPFGSTSIYAQYRVFKLAQQNGVTVTLDGQGADEMMGGYNGYPGQRMLSLLDkgayvqaltflr 407
                                                ************************************************99999888777666666555 PP

                                  TIGR01536 385 ....lpeaselaekkl..................llqaklakeselkellkakleeelkekeelkkel 430
                                                                                    ++ l++ + ++++  +  +  l+e     ++ 
  lcl|FitnessBrowser__HerbieS:HSERO_RS21075 408 nwaaW----------PgrspieglkrlvgagssgQFNSILRRLNGMQNIPDWIRPGPLREAGVRLSYP 465
                                                55440..........03333444444555666656666666666666666666666666655555555 PP

                                  TIGR01536 431 kee........seleellrldlelllsdllrakDrvsmahslEvRvPflDkelvelalsippelklr. 489
                                                +++        + ++ l ++ +e+ l +llr  Dr sm  s+E+RvPfl   lve++l+ p e+ ++ 
  lcl|FitnessBrowser__HerbieS:HSERO_RS21075 466 QAQpiapdhgrRMMAFLAQSLTERGLLGLLRHGDRNSMRFSVESRVPFLTTGLVEFMLRQPEEYLVSp 533
                                                555777867755555555666667777888888*********************************** PP

                                  TIGR01536 490 dgkeKvlLreaaeellPeeileR.kKeaf 517
                                                +g++K lLr  +++++P+++l+R +K +f
  lcl|FitnessBrowser__HerbieS:HSERO_RS21075 534 SGETKALLRASMRGIVPDAVLDRrDKIGF 562
                                                ***********************999888 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (517 nodes)
Target sequences:                          1  (627 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02
# Mc/sec: 13.55
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis