Align Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 (characterized)
to candidate HSERO_RS00990 HSERO_RS00990 aspartyl-tRNA synthetase
Query= SwissProt::Q51422 (591 letters) >FitnessBrowser__HerbieS:HSERO_RS00990 Length = 601 Score = 759 bits (1961), Expect = 0.0 Identities = 385/597 (64%), Positives = 459/597 (76%), Gaps = 9/597 (1%) Query: 2 MRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFAKA 61 MR+ YCG E+L GQ V+LCGWVHRRRDHGGVIF+D+RDREGL QVV DPDRA+ F A Sbjct: 3 MRTQYCGLTTEALLGQTVSLCGWVHRRRDHGGVIFIDLRDREGLVQVVCDPDRADVFKNA 62 Query: 62 DRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELEVLNQAETPPFPLDEYSDVGE 121 + VR+EF ++ITG VR RPEG N N+ SG IEVL +ELEVLN + TPPF LD+ S + E Sbjct: 63 ESVRNEFCLRITGLVRNRPEGTTNANLKSGKIEVLCHELEVLNPSVTPPFQLDDDS-LSE 121 Query: 122 ETRLRYRFIDLRRPEMAAKLKLRARITSSIRRYLDDNGFLDVETPILGRPTPEGARDYLV 181 TRL +R +DLRRP+M L+LR ++ +R++LD GF+D+ETP+L + TPEGARDYLV Sbjct: 122 TTRLTHRVLDLRRPQMQNNLRLRYKVAMEVRKFLDAQGFIDIETPMLTKSTPEGARDYLV 181 Query: 182 PSRTYPGHFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSF 241 PSR G FFALPQSPQLFKQLLMVA FDRYYQI KCFRDEDLRADRQPEFTQID ETSF Sbjct: 182 PSRVNAGEFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETSF 241 Query: 242 LDESDIIGITEKMVRQLFKEVLDVEF-DEFPHMPFEEAMRRYGSDKPDLRIPLELVDVAD 300 L+E +I + E M+R +FK LD++ + FP M F AM +YGSDKPD+R+ LE ++ D Sbjct: 242 LNEQEIRDLFEGMIRLVFKNALDIDLPNPFPVMDFATAMGKYGSDKPDMRVKLEFTELTD 301 Query: 301 QLKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKGLAYIKVNER 360 +K+VEFKVFSG AN GRV LRVPG A MPRS+ID YT+FV IYGAKGLAYIKVNE+ Sbjct: 302 VMKDVEFKVFSGAANSEGGRVVGLRVPGGAEMPRSEIDAYTQFVAIYGAKGLAYIKVNEK 361 Query: 361 AKGVEGLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADKAKIVCDALGALRIKVGHD- 419 AKG +GLQSPIVK I +A LN IL++ GA DGD++FFGADKAK+V DA+GALR+K+GH Sbjct: 362 AKGRDGLQSPIVKNIHDAALNAILEKTGAQDGDLIFFGADKAKVVNDAIGALRVKIGHSE 421 Query: 420 ----LKLLTREWAPMWVVDFPMFEENDDGS-LSALHHPFTSPKCTPAE-LEANPGAALSR 473 L W P+WVVDFPMFE ++DG ALHHPFTSPK + + NPGAA+++ Sbjct: 422 FGKKNGLFDDAWRPLWVVDFPMFEYDEDGQRWVALHHPFTSPKDGHEDFISTNPGAAIAK 481 Query: 474 AYDMVLNGTELGGGSIRIHDKSMQQAVFRVLGIDEAEQEEKFGFLLDALKYGAPPHGGLA 533 AYDMVLNG ELGGGS+RIH +Q VFR L ID+ E + KFGFLLDAL+YGAPPHGG+A Sbjct: 482 AYDMVLNGWELGGGSVRIHRADVQSKVFRALKIDDEEAKLKFGFLLDALQYGAPPHGGIA 541 Query: 534 FGLDRLVMLMTGASSIREVIAFPKTQSAGDVMTQAPGSVDGKALRELHIRLREQPKA 590 FGLDRLV +M GA SIR+VIAFPKTQ A ++T AP VD K LRELHIRLR A Sbjct: 542 FGLDRLVTMMAGAESIRDVIAFPKTQRAQCLLTHAPSEVDEKQLRELHIRLRNTEPA 598 Lambda K H 0.321 0.140 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1108 Number of extensions: 48 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 591 Length of database: 601 Length adjustment: 37 Effective length of query: 554 Effective length of database: 564 Effective search space: 312456 Effective search space used: 312456 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory