Alignments for a candidate for cysK in Herbaspirillum seropedicae SmR1

GapMind for Amino acid biosynthesis

Alignments for a candidate for cysK in Herbaspirillum seropedicae SmR1

Align S-sulfo-L-cysteine synthase (O-acetyl-L-serine-dependent) (EC 2.5.1.144); cysteine synthase (EC 2.5.1.47) (characterized)
to candidate HSERO_RS04915 HSERO_RS04915 cysteine synthase

Query= BRENDA::P29848
         (303 letters)



>FitnessBrowser__HerbieS:HSERO_RS04915
          Length = 300

 Score =  382 bits (980), Expect = e-111
 Identities = 189/294 (64%), Positives = 227/294 (77%), Gaps = 4/294 (1%)

Query: 3   TLEQTIGNTPLVKLQRLGPDNGSE----IWVKLEGNNPAGSVKDRAALSMIVEAEKRGEI 58
           T+E TIGNTPL+ L R+G +        I  KLEGNNPAGSVKDRAA SMI  AE RG+I
Sbjct: 5   TIESTIGNTPLILLPRIGGEEAKRRNNLILGKLEGNNPAGSVKDRAAFSMITRAEARGQI 64

Query: 59  KPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAAMRAYGAELILVTKEQG 118
           KPGD LIEATSGNTGIALAM+AA++GY+M LLMP+N+S+ERR +M AYGA+++L  K  G
Sbjct: 65  KPGDTLIEATSGNTGIALAMVAAMRGYKMILLMPENLSEERRQSMAAYGAKIVLTPKSGG 124

Query: 119 MEGARDLALAMSERGEGKLLDQFNNPDNPYAHYTTTGPEIWRQTSGRITHFVSSMGTTGT 178
           ME ARDLA  M + GEG +LDQF NPDNP AHY TTGPEIWR T G ITHFVS+MGTTGT
Sbjct: 125 MEYARDLAEQMQKNGEGLILDQFANPDNPLAHYETTGPEIWRDTKGSITHFVSAMGTTGT 184

Query: 179 ITGVSRFLREQEKPVTIVGLQPEEGSSIPGIRRWPAEYMPGIFNASLVDEVLDIHQNDAE 238
           I GV+++L+EQ   V IVG QPEEGSSIPGIR+WP  Y+P IF+ S VD++  + Q DAE
Sbjct: 185 IMGVAQYLKEQNAQVQIVGAQPEEGSSIPGIRKWPEAYLPKIFDRSRVDQIESVSQADAE 244

Query: 239 NTMRELAVREGIFCGVSSGGAVAGALRVARATPGAIVVAIICDRGDRYLSTGVF 292
           N  R+LAV EG+FCG+S+ GA   A+R++     A +V I+CDRGDRYLSTGVF
Sbjct: 245 NMARKLAVTEGVFCGISAAGACEVAVRLSHQLENATIVFIVCDRGDRYLSTGVF 298


Lambda     K      H
   0.316    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 333
Number of extensions: 14
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 303
Length of database: 300
Length adjustment: 27
Effective length of query: 276
Effective length of database: 273
Effective search space:    75348
Effective search space used:    75348
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 48 (23.1 bits)

Align candidate HSERO_RS04915 HSERO_RS04915 (cysteine synthase)
to HMM TIGR01138 (cysM: cysteine synthase B (EC 2.5.1.47))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01138.hmm
# target sequence database:        /tmp/gapView.10260.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01138  [M=290]
Accession:   TIGR01138
Description: cysM: cysteine synthase B
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                  Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                  -----------
   1.7e-139  450.3   0.2   1.9e-139  450.1   0.2    1.0  1  lcl|FitnessBrowser__HerbieS:HSERO_RS04915  HSERO_RS04915 cysteine synthase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__HerbieS:HSERO_RS04915  HSERO_RS04915 cysteine synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  450.1   0.2  1.9e-139  1.9e-139       1     290 []       5     298 ..       5     298 .. 0.99

  Alignments for each domain:
  == domain 1  score: 450.1 bits;  conditional E-value: 1.9e-139
                                  TIGR01138   1 tilklvGntplvrlkrllpe....edsevlvklegnnpaGsvkdrpalsmiveaekrGeikeGdvlie 64 
                                                ti+ ++Gntpl+ l r+++e     ++ +l klegnnpaGsvkdr+a+smi++ae rG+ik+Gd+lie
  lcl|FitnessBrowser__HerbieS:HSERO_RS04915   5 TIESTIGNTPLILLPRIGGEeakrRNNLILGKLEGNNPAGSVKDRAAFSMITRAEARGQIKPGDTLIE 72 
                                                899****************977766778999************************************* PP

                                  TIGR01138  65 atsGntGialamvaalkGykvkllmpdnvseerkaalkayGaelilvdkeeGmeGardlarelvrkge 132
                                                atsGntGialamvaa++Gyk++llmp+n seer++ + ayGa+++l+ k  Gme ardla++++++ge
  lcl|FitnessBrowser__HerbieS:HSERO_RS04915  73 ATSGNTGIALAMVAAMRGYKMILLMPENLSEERRQSMAAYGAKIVLTPKSGGMEYARDLAEQMQKNGE 140
                                                ******************************************************************** PP

                                  TIGR01138 133 eklldqfnnpdnpkahytstGieiwqqtkGrithfvsslGttGtimGvsrflkeqnpavqivGlqpae 200
                                                + +ldqf+npdnp ahy +tG+eiw+ tkG+ithfvs++GttGtimGv+++lkeqn +vqivG qp+e
  lcl|FitnessBrowser__HerbieS:HSERO_RS04915 141 GLILDQFANPDNPLAHYETTGPEIWRDTKGSITHFVSAMGTTGTIMGVAQYLKEQNAQVQIVGAQPEE 208
                                                ******************************************************************** PP

                                  TIGR01138 201 gsaieGlrrieseylpgifdaslvdrvvdveqedaediarelakkegifvGvssGgavaaalrlarel 268
                                                gs+i+G+r++++ ylp+ifd s+vd++  v q dae++ar+la+ eg+f+G+s+ ga   a+rl+++l
  lcl|FitnessBrowser__HerbieS:HSERO_RS04915 209 GSSIPGIRKWPEAYLPKIFDRSRVDQIESVSQADAENMARKLAVTEGVFCGISAAGACEVAVRLSHQL 276
                                                ******************************************************************** PP

                                  TIGR01138 269 ekavvvaiicdrGdrylstgvf 290
                                                e+a +v i+cdrGdrylstgvf
  lcl|FitnessBrowser__HerbieS:HSERO_RS04915 277 ENATIVFIVCDRGDRYLSTGVF 298
                                                *********************8 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (290 nodes)
Target sequences:                          1  (300 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 8.15
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis