Alignments for a candidate for gltX in Herbaspirillum seropedicae SmR1

GapMind for Amino acid biosynthesis

Alignments for a candidate for gltX in Herbaspirillum seropedicae SmR1

Align glutamyl-tRNA(Glx) synthetase (EC 6.1.1.24) (characterized)
to candidate HSERO_RS15460 HSERO_RS15460 glutamyl-tRNA synthetase

Query= reanno::Caulo:CCNA_01982
         (470 letters)



>FitnessBrowser__HerbieS:HSERO_RS15460
          Length = 466

 Score =  374 bits (959), Expect = e-108
 Identities = 202/469 (43%), Positives = 277/469 (59%), Gaps = 11/469 (2%)

Query: 5   TPTGVVTRFAPSPTGFLHIGGARTALFNWLYARHTGGKFLIRVEDTDRERSTEAAVAAIF 64
           T T V TRFAPSPTG+LH+GGARTALF+W YAR  GG F++R+EDTD ERST  AV AI 
Sbjct: 2   TATQVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAII 61

Query: 65  EGLDWLGLKSDDEVIFQHTRAPRHVEVVHELLAKGRAYRCWMSIEELEVAREKARAEGRA 124
           EG+ WLGL+ D+   +Q  R  R+ EV+ ++L +G AY C+ + EE+E  RE+ RA G  
Sbjct: 62  EGMQWLGLEHDEGPFYQMKRMDRYREVIAQMLEQGTAYYCYCTPEEVEAMRERQRAAGEK 121

Query: 125 IR--SPWRDAPEGDLSA-----PHVIRFKGPLDGETLVNDLVKGPVTFKNIELDDLVLLR 177
            R    WR  P   L A       V+RFK P DGE   ND+VKGP+T  N ELDDLV+ R
Sbjct: 122 PRYDGTWRPEPGKTLPAIPEGRKPVVRFKNPQDGEVSWNDVVKGPITISNRELDDLVIAR 181

Query: 178 ADGAPTYNLAVVVDDHDMGVTHVIRGDDHLNNAARQTLIYQAMDWAVPAFAHIPLIHGPD 237
            DG PTYN  VVVDD DM +THVIRGDDH+NN  RQ  I +A+   +P + H+P+I G D
Sbjct: 182 PDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKALGGTLPEYGHVPMILGAD 241

Query: 238 GAKLSKRHGAQAVGEFADLGYIPEGMRNYLARLGWGHGDDEVFTDEQAISWFDVADVVKA 297
           G KLSKRHGA +V ++   GY+PE M NYLARLGW HGDDE+F+ +Q  +WFD+  + KA
Sbjct: 242 GEKLSKRHGAVSVMDYPAQGYLPEAMLNYLARLGWSHGDDEIFSMQQFCAWFDLDHLTKA 301

Query: 298 PARLDWAKLNHINAQHLRKADDARLTALALAAAETRGEPLPADAAERIARTVPEVKEGAK 357
           PA+ +  KLN +N  +++ AD+ARL  L     E +G     + A  +A  +  +K+ A 
Sbjct: 302 PAQFNPEKLNWLNNHYIKLADNARLAGLVRPLLEAQGAVF--EGAPDLAAVIALLKDRAN 359

Query: 358 TILELVDHCAFALKTRPLALEEKTQKQLTEETVERLKRLRDQLAAAPDFDAATLETVLKS 417
           T+ E+    A     +P        + +TE     L     ++A   ++    +  ++K 
Sbjct: 360 TVNEIA-AAALMFYRQPAPDAALVAQHITEAIKPALADFAQRVATV-EWTKEAIAPMIKE 417

Query: 418 FAESEGVGFGKFGPALRGVLTGGAQAPDLNKTMAALSRDEAIGRLDDAL 466
              + G+   +    LR ++ G  Q P ++  +    R+    RL   L
Sbjct: 418 VLAAHGIKMPQLAMPLRLIVAGQLQTPAIDAVLQLFGREAVQARLSGYL 466


Lambda     K      H
   0.319    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 678
Number of extensions: 35
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 470
Length of database: 466
Length adjustment: 33
Effective length of query: 437
Effective length of database: 433
Effective search space:   189221
Effective search space used:   189221
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis