GapMind for Amino acid biosynthesis

 

Alignments for a candidate for leuD in Herbaspirillum seropedicae SmR1

Align 3-isopropylmalate dehydratase small subunit; EC 4.2.1.33; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase (uncharacterized)
to candidate HSERO_RS11425 HSERO_RS11425 aconitate hydratase

Query= curated2:C1CVE0
         (181 letters)



>FitnessBrowser__HerbieS:HSERO_RS11425
          Length = 873

 Score = 53.5 bits (127), Expect = 1e-11
 Identities = 38/90 (42%), Positives = 49/90 (54%), Gaps = 8/90 (8%)

Query: 43  FAKRVQPGDIIVAGADFGCGSSREHAVWALRGAGVGAVIAPNFARIYYRNSINNGFLALE 102
           + +R QP  II+AGAD+G GSSR+ A   +R AGV A++A  F RI+  N I  G L LE
Sbjct: 728 YMERKQPL-IIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLE 786

Query: 103 CDGIVEAFQDGDEANLDLKGGTITNLRTGQ 132
                  F+ G+  N     GT T    GQ
Sbjct: 787 -------FKAGENRNTYAIDGTETYDVIGQ 809


Lambda     K      H
   0.319    0.136    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 374
Number of extensions: 20
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 181
Length of database: 873
Length adjustment: 30
Effective length of query: 151
Effective length of database: 843
Effective search space:   127293
Effective search space used:   127293
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory