Align 3-isopropylmalate dehydratase small subunit; EC 4.2.1.33; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase (uncharacterized)
to candidate HSERO_RS14940 HSERO_RS14940 aconitate hydratase
Query= curated2:Q5JFV7 (163 letters) >FitnessBrowser__HerbieS:HSERO_RS14940 Length = 903 Score = 49.3 bits (116), Expect = 2e-10 Identities = 44/125 (35%), Positives = 61/125 (48%), Gaps = 21/125 (16%) Query: 54 VVVGGRNFGIGSSRESAALSLKAAGVAGVIAESFGRIFYRNAVNLG-LPL-LVGDTS--- 108 +V GG +G GSSR+ AA + GV VIA SF RI N V +G LPL +G+ S Sbjct: 775 MVFGGEEYGTGSSRDWAAKGTQLLGVKAVIARSFERIHRSNLVGMGVLPLQFLGNDSVQS 834 Query: 109 -GLRDGEVVEVNWRTGEVRTENG---VFHFKPLD----GFLLRI--------VEEGGILS 152 G+ E ++ GE++ + V H K + LLRI + GGIL Sbjct: 835 LGITGDETFDLKGLEGEIKPQQEATLVIHRKNGETKEVKLLLRIDTPIEVDYYKHGGILP 894 Query: 153 FIARR 157 F+ R+ Sbjct: 895 FVLRQ 899 Lambda K H 0.321 0.142 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 380 Number of extensions: 19 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 1 Length of query: 163 Length of database: 903 Length adjustment: 30 Effective length of query: 133 Effective length of database: 873 Effective search space: 116109 Effective search space used: 116109 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory