Aligments for a candidate for DAPtransferase in Herbaspirillum seropedicae SmR1

GapMind for Amino acid biosynthesis

Aligments for a candidate for DAPtransferase in Herbaspirillum seropedicae SmR1

Align LL-diaminopimelate aminotransferase (EC 2.6.1.83) (characterized)
to candidate HSERO_RS03055 HSERO_RS03055 inorganic polyphosphate/ATP-NAD kinase

Query= BRENDA::A3DDM2
         (289 letters)



>FitnessBrowser__HerbieS:HSERO_RS03055
          Length = 305

 Score =  154 bits (390), Expect = 2e-42
 Identities = 88/250 (35%), Positives = 146/250 (58%), Gaps = 5/250 (2%)

Query: 33  AIVCDDIALKLGDKESNINEDNIVDMSDVMVCLGGDGTFLKAARMTVVKGKPLLGVNLGK 92
           A   +++AL+  D   ++  + I   +DV + +GGDGT L  AR       PL+G+N G+
Sbjct: 49  AETAENVALQGYD---SLTTEQIGQHADVAIVVGGDGTMLGIARQLAPYNVPLIGINQGR 105

Query: 93  LGFLADVDKNDIENAVKRLVEDKFTVDERMMLDTVIVRDGKIIAEDIVLNDVVISRGAIS 152
           LGF+ D+ ++ +  A+  ++E K   + R +L+  + R+G  I   + LNDVV++RG+ S
Sbjct: 106 LGFITDIAQDRMIPALADMLEGKVEAESRSLLEARVYREGGEIFRALALNDVVVARGSTS 165

Query: 153 RILHLKTYINDAFMDLYPGDGLIISTPTGSTAYSLSAGGPLVEPDVDLIICTPICPHLLY 212
            ++ L+  ++  FM     DGLI++TPTGSTAY+LSAGGP++ P +  I+  PI PH L 
Sbjct: 166 GMVELRVEVDGRFMYNQRSDGLIVATPTGSTAYALSAGGPILHPSLHGIVMVPISPHSLS 225

Query: 213 SRSFITTADRVIKVVVAESSSHEAMVTVDGQNGYEVRGGDVIITKKSRIRMPMVRLNGKN 272
           +R  IT +D   ++V+   S  E     D Q+   V  GD I+ ++S  ++  +   G +
Sbjct: 226 NRP-ITLSDS-CEIVIQVVSGREVSANFDMQSLTSVLHGDRIVIRRSAHKITFLHPQGWS 283

Query: 273 FFDVLRGKIY 282
           +FD LR K++
Sbjct: 284 YFDTLREKLH 293


Lambda     K      H
   0.320    0.140    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 216
Number of extensions: 9
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 289
Length of database: 305
Length adjustment: 26
Effective length of query: 263
Effective length of database: 279
Effective search space:    73377
Effective search space used:    73377
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis