Align 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7) (characterized)
to candidate HSERO_RS16395 HSERO_RS16395 dihydrodipicolinate synthase
Query= BRENDA::Q9I4W3 (292 letters) >FitnessBrowser__HerbieS:HSERO_RS16395 Length = 292 Score = 352 bits (902), Expect = e-102 Identities = 172/291 (59%), Positives = 219/291 (75%) Query: 1 MIAGSMVALVTPFDAQGRLDWDSLAKLVDFHLQEGTNAIVAVGTTGESATLDVEEHIQVI 60 MI GS+VA+VTP G LD+D L KL+D+H+ EGT++IV VGTTGES T+ +EEH ++I Sbjct: 1 MIKGSIVAIVTPMHQDGSLDYDGLRKLIDWHIAEGTDSIVIVGTTGESPTVSMEEHCELI 60 Query: 61 RRVVDQVKGRIPVIAGTGANSTREAVALTEAAKSGGADACLLVTPYYNKPTQEGMYQHFR 120 + V+ RIP+IAGTG NST EA+ LTE AK GADA L V PYYN+PTQEGMY HF+ Sbjct: 61 KVAVEHTAKRIPIIAGTGGNSTSEAIELTEFAKKVGADASLQVVPYYNRPTQEGMYLHFK 120 Query: 121 HIAEAVAIPQILYNVPGRTSCDMLPETVERLSKVPNIIGIKEATGDLQRAKEVIERVGKD 180 IAE+V +P ILYNVPGRT DM ET+ RL++V I+G+K+ATG++ R ++I K Sbjct: 121 KIAESVDLPVILYNVPGRTVADMSNETILRLAQVKGIVGVKDATGNIARGTDLIRLAPKS 180 Query: 181 FLVYSGDDATAVELMLLGGKGNISVTANVAPRAMSDLCAAAMRGDAAAARAINDRLMPLH 240 F VYSGDDATA+ LML GG GNISVTANVAPR M +LC AAM G+ A A +N++L+PLH Sbjct: 181 FAVYSGDDATAIALMLYGGAGNISVTANVAPRDMHELCVAAMSGNIARAVELNNKLLPLH 240 Query: 241 KALFIESNPIPVKWALHEMGLIPEGIRLPLTWLSPRCHEPLRQAMRQTGVL 291 LF+E NP+P+KWA+ EMGLIP G+RLPL L H+ +R A+R++GVL Sbjct: 241 NKLFVEPNPVPLKWAMQEMGLIPSGMRLPLAPLGAAYHDTVRAALRESGVL 291 Lambda K H 0.319 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 292 Number of extensions: 13 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 292 Length of database: 292 Length adjustment: 26 Effective length of query: 266 Effective length of database: 266 Effective search space: 70756 Effective search space used: 70756 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
Align candidate HSERO_RS16395 HSERO_RS16395 (dihydrodipicolinate synthase)
to HMM TIGR00674 (dapA: 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00674.hmm # target sequence database: /tmp/gapView.11346.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00674 [M=286] Accession: TIGR00674 Description: dapA: 4-hydroxy-tetrahydrodipicolinate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.1e-115 369.9 0.0 3.4e-115 369.8 0.0 1.0 1 lcl|FitnessBrowser__HerbieS:HSERO_RS16395 HSERO_RS16395 dihydrodipicolinat Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__HerbieS:HSERO_RS16395 HSERO_RS16395 dihydrodipicolinate synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 369.8 0.0 3.4e-115 3.4e-115 1 284 [. 4 286 .. 4 288 .. 0.99 Alignments for each domain: == domain 1 score: 369.8 bits; conditional E-value: 3.4e-115 TIGR00674 1 gvltAliTPfkedgsvdfaalekliesqiekgvdaivvvGtTGEsatLsleEkkkvievavelvknrv 68 g+++A++TP+++dgs+d++ l kli+ +i++g+d+iv+vGtTGEs+t s+eE+ ++i+vave +++r+ lcl|FitnessBrowser__HerbieS:HSERO_RS16395 4 GSIVAIVTPMHQDGSLDYDGLRKLIDWHIAEGTDSIVIVGTTGESPTVSMEEHCELIKVAVEHTAKRI 71 589***************************************************************** PP TIGR00674 69 pviaGtgsnateeaieltkeaeklgvdgvlvvtPyYnkPtqeGlykhfkaiaeevelPiilYnvPsRt 136 p+iaGtg+n+t+eaielt++a+k+g+d+ l+v+PyYn+PtqeG+y hfk+iae+v+lP+ilYnvP+Rt lcl|FitnessBrowser__HerbieS:HSERO_RS16395 72 PIIAGTGGNSTSEAIELTEFAKKVGADASLQVVPYYNRPTQEGMYLHFKKIAESVDLPVILYNVPGRT 139 ******************************************************************** PP TIGR00674 137 gvslepetvkrLaeeveivaiKeasgdlervseikaeakedfkvlsGdDaltleilalGakGviSVas 204 +++++ et++rLa+ + iv++K+a+g+++r + +++ a+++f+v+sGdDa++ ++++ G++G iSV++ lcl|FitnessBrowser__HerbieS:HSERO_RS16395 140 VADMSNETILRLAQVKGIVGVKDATGNIARGTDLIRLAPKSFAVYSGDDATAIALMLYGGAGNISVTA 207 ***************9**************************************************** PP TIGR00674 205 nvapkelkemvkaalegdteeareihqkllklfkalfietNPipvKtalallgliekdelRlPLtels 272 nvap++++e++ aa+ g++++a e+++kll+l+++lf+e+NP+p+K+a++ +gli + +RlPL++l lcl|FitnessBrowser__HerbieS:HSERO_RS16395 208 NVAPRDMHELCVAAMSGNIARAVELNNKLLPLHNKLFVEPNPVPLKWAMQEMGLIPS-GMRLPLAPLG 274 *********************************************************.*********9 PP TIGR00674 273 eekkeklkevlk 284 +++++++l+ lcl|FitnessBrowser__HerbieS:HSERO_RS16395 275 AAYHDTVRAALR 286 999999999887 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (286 nodes) Target sequences: 1 (292 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 7.42 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory