Align Putative acetolactate synthase large subunit IlvX; ALS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; AHAS (uncharacterized)
to candidate HSERO_RS19735 HSERO_RS19735 acetolactate synthase
Query= curated2:O53554 (515 letters) >FitnessBrowser__HerbieS:HSERO_RS19735 Length = 518 Score = 382 bits (982), Expect = e-110 Identities = 233/519 (44%), Positives = 301/519 (57%), Gaps = 17/519 (3%) Query: 3 GAQALINTLVDGGVDVCFANPGTSEMHFVAALDAVPRMRGMLTLFEGVATGAADGYARIA 62 GA AL L+D GVD CFANPGT+E+ V L++ P++R ++ L E V TGAADGY R+ Sbjct: 6 GAHALWAGLLDQGVDTCFANPGTTELDLVRGLESFPKLRCVVALQENVCTGAADGYGRML 65 Query: 63 GRPAAVLLHLGPGLGNGLANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDAVAGTV 122 GRPA+ LLHLGPG NG+ANLHNARRAR P+V ++GDH ++H YDAPL SDI+++A V Sbjct: 66 GRPASTLLHLGPGFANGIANLHNARRARTPIVNIIGDHTSWHLPYDAPLSSDIESLAHPV 125 Query: 123 SGWVRRTEAAADV-GADAEAAIAASRSGSQIATLILPADVCWSDGAHAAAGVPAQAAAAP 181 SGWV R AD+ A AE+ ++ +G Q +TLI PAD ++D A A+ + Sbjct: 126 SGWVHRITGTADIAAAAAESVRQSTAAGGQGSTLIFPADFQYADMAEASPAAATEKDTGQ 185 Query: 182 VD---VGPVAGVLRSGEPAMMLIGGDATRGPGL-----TAAARIVQATGARWLCETFPTC 233 D L+ + + L+GG GL A ARI G ETFP Sbjct: 186 ADDYRADQALARLQGAQRVVFLLGGGGEES-GLGARAQRAVARIAAHIGCSVYAETFPAR 244 Query: 234 LERGAGIPAVERLAYFAEGAAAQLDGVKHLVLAGARSPVSFFAYPGMPSDLVPAGCEVHV 293 ERG G+P+ +RL YF E A LD +VL GA P+++F Y G PS LV A + Sbjct: 245 AERGQGLPSFDRLPYFPEPARKVLDDADLVVLVGALPPITYFGYVGHPSALV-APERLLT 303 Query: 294 LAEPG-GAADALAALADEV-APGTVAPVAGASRPQLPTGDLTSVSAADVVGALLPERAIV 351 ++ PG AA + LAD + AP V P + P LP G+LT A V+ LPERAIV Sbjct: 304 ISLPGRPAAGRVEQLADALGAPAYVTP--QHALPPLPEGELTPQKIAAVLARELPERAIV 361 Query: 352 VDESNTCGVLLPQATAGAPAHDWLTLTGGAIGYGIPAAVGAAVAAPDRPVLCLESDGSAM 411 E TCG + A H LT TGGAIG G+P A+GAAVA PDR V+ L SDGS Sbjct: 362 SVEGGTCGYPFYAVSDAARPHTVLTNTGGAIGQGLPVALGAAVACPDRTVVGLLSDGSTQ 421 Query: 412 YTISGLWSQARENLDVTTVIYNNGAYDILRIELQRVGAGSDPGPKALDLLDISRPTMDFV 471 YTI LWS A EN V +I N Y ILR EL+R GA G +A + + P +D+V Sbjct: 422 YTIQTLWSLAHENARVVVLIAANHQYAILRNELRRDGA--PLGERAAQMTALDHPRIDWV 479 Query: 472 KIAEGMGVPARRVTTCEEFADALRAAFAEPGPHLIDVVV 510 +A+ GVPA R T ++ L+AAFA GP LI++ + Sbjct: 480 GLAQSYGVPASRAETAQQLQQQLQAAFAGTGPALIEMAL 518 Lambda K H 0.318 0.134 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 636 Number of extensions: 24 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 515 Length of database: 518 Length adjustment: 35 Effective length of query: 480 Effective length of database: 483 Effective search space: 231840 Effective search space used: 231840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory