Alignments for a candidate for aceA in Sphingomonas koreensis DSMZ 15582

GapMind for Amino acid biosynthesis

Alignments for a candidate for aceA in Sphingomonas koreensis DSMZ 15582

Align isocitrate lyase (EC 4.1.3.1) (characterized)
to candidate Ga0059261_1963 Ga0059261_1963 Isocitrate lyase

Query= BRENDA::Q9I0K4
         (531 letters)



>FitnessBrowser__Korea:Ga0059261_1963
          Length = 531

 Score =  846 bits (2185), Expect = 0.0
 Identities = 422/528 (79%), Positives = 460/528 (87%), Gaps = 2/528 (0%)

Query: 4   YQNEI-KAVAALKEKNGSSWSAINPEYAARMRIQNRFKTGLDIAKYTAAIMRKDMAEYDA 62
           YQ+EI +A A +  +NG  W+ I+ E  ARMR+QNRFKTGLDIAKYTA IMR+DMA YDA
Sbjct: 3   YQDEIARANAPIAAENGR-WNGIDAESVARMRLQNRFKTGLDIAKYTAGIMRRDMAAYDA 61

Query: 63  DSSVYTQSLGCWHGFIGQQKLISIKKHLKTTNKRYLYLSGWMVAALRSDFGPLPDQSMHE 122
           D S YTQSLGCWHGFI QQKLISIKKH  TT  RYLYLSGWMVAALRSDFGPLPDQSMHE
Sbjct: 62  DPSAYTQSLGCWHGFIAQQKLISIKKHFGTTKGRYLYLSGWMVAALRSDFGPLPDQSMHE 121

Query: 123 KTAVSGLIEELYTFLRQADARELDLLFTGLDAARAAGDKAKEAELLAQIDNFETHVVPII 182
           KT+V  LIEELYTFL+QADAREL  LF  LD AR  G++ + A +   IDNFETHVVPII
Sbjct: 122 KTSVPALIEELYTFLKQADARELGGLFRDLDKAREGGNEVEAARIQNAIDNFETHVVPII 181

Query: 183 ADIDAGFGNAEATYLLAKKMIEAGACCIQIENQVSDEKQCGHQDGKVTVPHIDFLAKINA 242
           ADIDAGFGNAEATYLLAKKMIEAGAC +QIENQVSDEKQCGHQDGKVTVPH DFLAK+ A
Sbjct: 182 ADIDAGFGNAEATYLLAKKMIEAGACALQIENQVSDEKQCGHQDGKVTVPHEDFLAKVRA 241

Query: 243 VRYAFLELGVDDGVIVARTDSLGAGLTKQIAVTNEPGDLGDLYNSFLDCEEISESELGNG 302
            RYAFLELGVDDG+IV RTDSLGAGLTKQIA + E GDLGD YN+FLDCEE+++     G
Sbjct: 242 CRYAFLELGVDDGIIVTRTDSLGAGLTKQIAYSKEKGDLGDQYNAFLDCEEVTDPATLRG 301

Query: 303 DVVIKREGKLLRPKRLASNLFQFRKGTGEDRCVLDCITSLQNGADLLWIETEKPHVGQIK 362
           DVVI+R+GKL+RPKRL SNLFQFR+GTG DRCVLDCITSLQ+GADLLWIETEKPH+ QI 
Sbjct: 302 DVVIERDGKLMRPKRLPSNLFQFREGTGADRCVLDCITSLQHGADLLWIETEKPHIEQIA 361

Query: 363 AMVDRIREVIPNAKLVYNNSPSFNWTLNFRQQVFDAFVAEGKDVSAYDRNKLMSVEYDDT 422
           +MVDRIREVIPNAKLVYNNSPSFNWTLNFRQQVFDA+ A GKDVSAYDR KLMSV+YD T
Sbjct: 362 SMVDRIREVIPNAKLVYNNSPSFNWTLNFRQQVFDAWEAAGKDVSAYDRAKLMSVDYDGT 421

Query: 423 ELAKVADEKIRTFQRDGSAHAGIFHHLITLPTYHTAALSTDNLAKGYFADEGMLAYVKGV 482
           +LA  ADEKIRTFQ+D +A AGIFHHLITLPTYHTAALSTDNLAK YF DE ML YVKGV
Sbjct: 422 DLAVEADEKIRTFQKDAAARAGIFHHLITLPTYHTAALSTDNLAKAYFGDEAMLGYVKGV 481

Query: 483 QRQELRQGIACVKHQNMAGSDIGDNHKEYFAGEAALKASGKDNTMNQF 530
           QR+E+RQGIACVKHQNM+GSDIGD+HKEYFAGEAALKA G  NTMNQF
Sbjct: 482 QREEIRQGIACVKHQNMSGSDIGDDHKEYFAGEAALKAGGVHNTMNQF 529


Lambda     K      H
   0.318    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 922
Number of extensions: 21
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 531
Length of database: 531
Length adjustment: 35
Effective length of query: 496
Effective length of database: 496
Effective search space:   246016
Effective search space used:   246016
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis