Alignments for a candidate for prs in Sphingomonas koreensis DSMZ 15582

GapMind for Amino acid biosynthesis

Alignments for a candidate for prs in Sphingomonas koreensis DSMZ 15582

Align ribose-phosphate diphosphokinase (EC 2.7.6.1) (characterized)
to candidate Ga0059261_2274 Ga0059261_2274 ribose-phosphate pyrophosphokinase

Query= BRENDA::P0A719
         (315 letters)



>FitnessBrowser__Korea:Ga0059261_2274
          Length = 311

 Score =  331 bits (848), Expect = 2e-95
 Identities = 171/312 (54%), Positives = 231/312 (74%), Gaps = 2/312 (0%)

Query: 4   MKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPT 63
           MKL AGN+   LAQ IA+ L   L  A V RF+D E+ V+I ENVRG D+F++QST  P 
Sbjct: 1   MKLMAGNSNLPLAQAIASYLEIPLTQANVRRFADEEIFVEILENVRGEDVFVLQSTAYPA 60

Query: 64  NDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVD 123
           NDNLMEL++M+DALRRASA RITAV+PYFGYARQDR+    R PI+AK+VA+ +++ G +
Sbjct: 61  NDNLMELLIMIDALRRASAKRITAVLPYFGYARQDRKP-GPRTPISAKLVANLITTAGAN 119

Query: 124 RVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLD-NPIVVSPDIGGVVRARAIAK 182
           RVL+VDLHA QIQGFFD+P DN+F +P++ ED+     D N +VVSPD+GGVVRARA+AK
Sbjct: 120 RVLSVDLHAGQIQGFFDIPTDNLFAAPVMSEDIKARFSDKNLMVVSPDVGGVVRARALAK 179

Query: 183 LLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERGAK 242
            L +  +AI+DKRR +A  S+VM+IIGDV GR C+L+DD++D+ GTLC AA ALK  GA+
Sbjct: 180 RLENAPLAIVDKRREKAGESEVMNIIGDVEGRFCILIDDIVDSAGTLCNAAAALKAAGAE 239

Query: 243 RVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAIRR 302
            V AY TH + SG A   +  S + E+V+ D+I     +     +R LT++ ++AEAIRR
Sbjct: 240 GVVAYCTHGVLSGGAVARVDGSELSELVITDSIGNHAIVGEAQRIRHLTIAPLIAEAIRR 299

Query: 303 ISNEESISAMFE 314
           I++E S+S++F+
Sbjct: 300 IADESSVSSLFD 311


Lambda     K      H
   0.321    0.137    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 301
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 315
Length of database: 311
Length adjustment: 27
Effective length of query: 288
Effective length of database: 284
Effective search space:    81792
Effective search space used:    81792
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate Ga0059261_2274 Ga0059261_2274 (ribose-phosphate pyrophosphokinase)
to HMM TIGR01251 (prs: ribose-phosphate diphosphokinase (EC 2.7.6.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01251.hmm
# target sequence database:        /tmp/gapView.2799.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01251  [M=309]
Accession:   TIGR01251
Description: ribP_PPkin: ribose-phosphate diphosphokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
     8e-118  378.8   0.1   8.9e-118  378.6   0.1    1.0  1  lcl|FitnessBrowser__Korea:Ga0059261_2274  Ga0059261_2274 ribose-phosphate 


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Korea:Ga0059261_2274  Ga0059261_2274 ribose-phosphate pyrophosphokinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  378.6   0.1  8.9e-118  8.9e-118       1     309 []       1     311 []       1     311 [] 0.98

  Alignments for each domain:
  == domain 1  score: 378.6 bits;  conditional E-value: 8.9e-118
                                 TIGR01251   1 lkilsgssskelaekvaknlglelgdvevkkFadgElyvrieesvrgkdvfiivqstsapvndalmell 69 
                                               +k+++g+s+  la+++a +l+++l++++v++Fad+E+ v+i e+vrg+dvf++ qst  p nd+lmell
  lcl|FitnessBrowser__Korea:Ga0059261_2274   1 MKLMAGNSNLPLAQAIASYLEIPLTQANVRRFADEEIFVEILENVRGEDVFVL-QSTAYPANDNLMELL 68 
                                               7899*************************************************.99999********** PP

                                 TIGR01251  70 llidalkrasaksvtaviPyygYaRqdkkaksrepisaklvaklleeaGadrvltvdlHseqiqgfFdv 138
                                               ++idal+rasak++tav+Py+gYaRqd+k  +r+pisaklva+l+++aGa+rvl vdlH+ qiqgfFd+
  lcl|FitnessBrowser__Korea:Ga0059261_2274  69 IMIDALRRASAKRITAVLPYFGYARQDRKPGPRTPISAKLVANLITTAGANRVLSVDLHAGQIQGFFDI 137
                                               ********************************************************************* PP

                                 TIGR01251 139 pvenlsaspklieelkkke.lknlvvvsPDkGaverakkvakklg.lelaiieKeRdskenevevtnll 205
                                               p +nl a+p++ e++k++  +knl+vvsPD G+v ra+++ak+l+ ++lai++K+R+ k+ e ev+n++
  lcl|FitnessBrowser__Korea:Ga0059261_2274 138 PTDNLFAAPVMSEDIKARFsDKNLMVVSPDVGGVVRARALAKRLEnAPLAIVDKRRE-KAGESEVMNII 205
                                               *****************999************************99***********.777******** PP

                                 TIGR01251 206 gdvegkdvvivDDiisTggTlvkaaelLkekGAkkvivaathgvfsgdAlerlaeagveevivtntilv 274
                                               gdveg+ ++++DDi++++gTl++aa +Lk +GA+ v++++thgv+sg A+ r++ + ++e+++t++i  
  lcl|FitnessBrowser__Korea:Ga0059261_2274 206 GDVEGRFCILIDDIVDSAGTLCNAAAALKAAGAEGVVAYCTHGVLSGGAVARVDGSELSELVITDSIGN 274
                                               *******************************************************************99 PP

                                 TIGR01251 275 ee..kklpkvseisvapliaeaiarihenesvsslfd 309
                                               +    + ++++++++apliaeai+ri++++svsslfd
  lcl|FitnessBrowser__Korea:Ga0059261_2274 275 HAivGEAQRIRHLTIAPLIAEAIRRIADESSVSSLFD 311
                                               5558999****************************96 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (309 nodes)
Target sequences:                          1  (311 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.54
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis