Align 3-isopropylmalate dehydratase large subunit; EC 4.2.1.33 (characterized)
to candidate Ga0059261_4223 Ga0059261_4223 3-isopropylmalate dehydratase, large subunit
Query= CharProtDB::CH_024771 (466 letters) >FitnessBrowser__Korea:Ga0059261_4223 Length = 478 Score = 597 bits (1538), Expect = e-175 Identities = 297/470 (63%), Positives = 367/470 (78%), Gaps = 9/470 (1%) Query: 4 TLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFATMDH 63 TLYEK++ AHVV ++ T L+YIDRHLVHEVTSPQAF+GLRA+GR VR+P T A DH Sbjct: 7 TLYEKIWAAHVVERRDDGTCLIYIDRHLVHEVTSPQAFEGLRANGRRVRRPDLTLAVPDH 66 Query: 64 NVSTQTKDINACGEMARI-------QMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQG 116 N+ T T ++A G I Q+ L +N EFG++ + QGIVHV+GPEQG Sbjct: 67 NLPT-TPRVDAAGNRLPIADRESAQQLAALERNVAEFGIDYFGATAAEQGIVHVVGPEQG 125 Query: 117 VTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKAAP 176 TLPG T+VCGDSHT+ HGA GALAFGIGTSEVEHVLATQTL ++KTM+I V G Sbjct: 126 FTLPGTTLVCGDSHTSAHGALGALAFGIGTSEVEHVLATQTLLLSQSKTMEIRVDGTLGF 185 Query: 177 GITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPD 236 G++AKD+VLAIIGKTG+AGGTG+VVE+ GE IR LS+EGR+T+ NM+IE GA++GL+APD Sbjct: 186 GVSAKDVVLAIIGKTGAAGGTGYVVEYTGEVIRALSIEGRLTVSNMSIEGGARSGLIAPD 245 Query: 237 ETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQ 296 E TF Y+KGR APKG+ ++ AVA+WKTL TD GA +D VVTL +I+P +TWGT+P Sbjct: 246 EKTFAYLKGRPMAPKGEQWEQAVAWWKTLPTDAGAQYDRVVTLNGSDIAPSLTWGTSPED 305 Query: 297 VISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFIGSCTNSRIEDLR 356 V+ + +PDP SFADP +R +A+K+L YMGL PG + ++A++ +FIGSCTNSRIEDLR Sbjct: 306 VVPITGVVPDPESFADPAKRVAAQKSLDYMGLAPGTRMQDIAVENIFIGSCTNSRIEDLR 365 Query: 357 AAAEIAKGRKVAPGV-QALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNN 415 AAA++ KGR VA G+ QAL+VPGSG VK QAEAEGLD+IF++AGFEWR PGCSMCLAMN Sbjct: 366 AAADVVKGRHVADGIRQALIVPGSGLVKRQAEAEGLDRIFLDAGFEWREPGCSMCLAMNP 425 Query: 416 DRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNI 465 D++ GERCASTSNRNF GRQG G RTHLVSPAMAAAAAVTG D+R++ Sbjct: 426 DKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGRLTDVRDL 475 Lambda K H 0.317 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 768 Number of extensions: 26 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 466 Length of database: 478 Length adjustment: 33 Effective length of query: 433 Effective length of database: 445 Effective search space: 192685 Effective search space used: 192685 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate Ga0059261_4223 Ga0059261_4223 (3-isopropylmalate dehydratase, large subunit)
to HMM TIGR00170 (leuC: 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00170.hmm # target sequence database: /tmp/gapView.9738.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00170 [M=466] Accession: TIGR00170 Description: leuC: 3-isopropylmalate dehydratase, large subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.4e-239 778.8 0.0 9.8e-239 778.5 0.0 1.0 1 lcl|FitnessBrowser__Korea:Ga0059261_4223 Ga0059261_4223 3-isopropylmalate Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Korea:Ga0059261_4223 Ga0059261_4223 3-isopropylmalate dehydratase, large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 778.5 0.0 9.8e-239 9.8e-239 2 466 .] 5 475 .. 4 475 .. 0.99 Alignments for each domain: == domain 1 score: 778.5 bits; conditional E-value: 9.8e-239 TIGR00170 2 aktlyeklfdahvvkeaenetdllyidrhlvhevtspqafeglraagrkvrrvdktlatldhnistesr 70 + tlyek++ ahvv++ +++t l+yidrhlvhevtspqafeglra+gr+vrr+d tla dhn++t+ r lcl|FitnessBrowser__Korea:Ga0059261_4223 5 PLTLYEKIWAAHVVERRDDGTCLIYIDRHLVHEVTSPQAFEGLRANGRRVRRPDLTLAVPDHNLPTTPR 73 569****************************************************************99 PP TIGR00170 71 ......dveikeekaklqvkeleknvkefgvklfdlssaeqgivhvvgpeegltlpgktivcgdshtat 133 + i + ++ q+ +le+nv efg+++f+ + aeqgivhvvgpe+g+tlpg+t+vcgdsht++ lcl|FitnessBrowser__Korea:Ga0059261_4223 74 vdaagnRLPIADRESAQQLAALERNVAEFGIDYFGATAAEQGIVHVVGPEQGFTLPGTTLVCGDSHTSA 142 98888778899********************************************************** PP TIGR00170 134 hgafgalafgigtsevehvlatqtlkqaraktlkievegklakgitakdiilaiigkigvaggtgyvve 202 hga+galafgigtsevehvlatqtl +++kt++i+v+g+l g++akd++laiigk+g+aggtgyvve lcl|FitnessBrowser__Korea:Ga0059261_4223 143 HGALGALAFGIGTSEVEHVLATQTLLLSQSKTMEIRVDGTLGFGVSAKDVVLAIIGKTGAAGGTGYVVE 211 ********************************************************************* PP TIGR00170 203 fageairdlsmeermtvcnmaieagakagliapdettfeyvkdrkyapkgkefekavaywktlktdega 271 ++ge+ir+ls+e+r+tv nm+ie ga++gliapde tf+y+k+r++apkg+++e+ava wktl td ga lcl|FitnessBrowser__Korea:Ga0059261_4223 212 YTGEVIRALSIEGRLTVSNMSIEGGARSGLIAPDEKTFAYLKGRPMAPKGEQWEQAVAWWKTLPTDAGA 280 ********************************************************************* PP TIGR00170 272 kfdkvvtleakdispqvtwgtnpgqvlsvneevpdpksladpvekasaekalaylglepgtklkdikvd 340 ++d+vvtl+++di+p +twgt+p++v+++++ vpdp+s+adp ++ a+k+l+y+gl pgt+++di+v+ lcl|FitnessBrowser__Korea:Ga0059261_4223 281 QYDRVVTLNGSDIAPSLTWGTSPEDVVPITGVVPDPESFADPAKRVAAQKSLDYMGLAPGTRMQDIAVE 349 ********************************************************************* PP TIGR00170 341 kvfigsctnsriedlraaaevvkgkkvadnvklalvvpgsglvkkqaekegldkifleagfewreagcs 409 ++figsctnsriedlraaa+vvkg++vad++++al+vpgsglvk+qae+egld+ifl+agfewre+gcs lcl|FitnessBrowser__Korea:Ga0059261_4223 350 NIFIGSCTNSRIEDLRAAADVVKGRHVADGIRQALIVPGSGLVKRQAEAEGLDRIFLDAGFEWREPGCS 418 ********************************************************************* PP TIGR00170 410 lclgmnndvldeyercastsnrnfegrqgkgarthlvspamaaaaavagkfvdirel 466 +cl+mn+d+++++ercastsnrnf grqg garthlvspamaaaaav+g++ d+r+l lcl|FitnessBrowser__Korea:Ga0059261_4223 419 MCLAMNPDKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGRLTDVRDL 475 *******************************************************85 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (466 nodes) Target sequences: 1 (478 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 10.56 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory