GapMind for Amino acid biosynthesis

 

Aligments for a candidate for proB in Sphingomonas koreensis DSMZ 15582

Align δ1-pyrroline-5-carboxylate synthetase (EC 1.2.1.41; EC 2.7.2.11) (characterized)
to candidate Ga0059261_2506 Ga0059261_2506 glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41)

Query= metacyc::AT2G39800-MONOMER
         (717 letters)



>lcl|FitnessBrowser__Korea:Ga0059261_2506 Ga0059261_2506
           glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41)
          Length = 422

 Score =  279 bits (713), Expect = 2e-79
 Identities = 159/408 (38%), Positives = 230/408 (56%), Gaps = 9/408 (2%)

Query: 297 DMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMV 356
           ++   AR ++  L  L +  + + L   A A+ A+   I A N  DVA+ +  GL  +++
Sbjct: 15  ELGARARAAAVTLAGLPAPAKAQGLRAAAAAIRADADAILAANARDVAAGEANGLSGALL 74

Query: 357 ARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRP 416
            RL + P ++   AA V  +A + DP+G V+  +E  +GL L +   P+GV+ I++ESRP
Sbjct: 75  DRLRLDPARLEGAAAGVEAVAALADPVGEVISTSERPNGLQLSRVRVPIGVIGIIYESRP 134

Query: 417 DALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPET-VGGKLIGLV--TSREE 473
           +     A+L + SGN  +L+GG EA  SN  +H      + +  +    + LV  T R  
Sbjct: 135 NVTADAAALCVMSGNAAILRGGSEAVHSNRAIHAAFACGLADAGLPEDAVQLVPTTDRAA 194

Query: 474 IPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVS 533
           +  +LK D  IDLV+PRG   LV +++   ++PVL H DGI H Y+D A D DMA+ +  
Sbjct: 195 VGAMLKADGAIDLVVPRGGKSLVARVQEEARVPVLAHLDGINHTYIDGAADPDMARDLAV 254

Query: 534 DAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKI---LNIPEA 590
           +AK+     C A ETLL+       A    ++ AL   G  L G      I   +    A
Sbjct: 255 NAKMRRTGICGATETLLIDTAFADPA---PILSALADTGCELRGDADIRAIDPRVVAANA 311

Query: 591 RSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFH 650
             ++ EY     +V+ V+ V GA+ HI  HGS HTD IVT D   AE FL  VDSA V  
Sbjct: 312 EDWDTEYLDSILSVKRVDGVVGAMAHIAAHGSHHTDAIVTGDAATAERFLNGVDSAIVMW 371

Query: 651 NASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQ 698
           NAST+F+DG  FGLGAE+G++TGR+HARGPV +EGL T +W++RG GQ
Sbjct: 372 NASTQFADGGEFGLGAEIGIATGRLHARGPVALEGLTTYKWVVRGTGQ 419


Lambda     K      H
   0.318    0.135    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 597
Number of extensions: 24
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 717
Length of database: 422
Length adjustment: 36
Effective length of query: 681
Effective length of database: 386
Effective search space:   262866
Effective search space used:   262866
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory