Aligments for a candidate for PAH in Sphingomonas koreensis DSMZ 15582

GapMind for Amino acid biosynthesis

Aligments for a candidate for PAH in Sphingomonas koreensis DSMZ 15582

Align phenylalanine 4-monooxygenase (EC 1.14.16.1) (characterized)
to candidate Ga0059261_1314 Ga0059261_1314 Phenylalanine 4-hydroxylase (EC 1.14.16.1)

Query= BRENDA::P30967
         (297 letters)



>lcl|FitnessBrowser__Korea:Ga0059261_1314 Ga0059261_1314
           Phenylalanine 4-hydroxylase (EC 1.14.16.1)
          Length = 288

 Score =  314 bits (804), Expect = 2e-90
 Identities = 154/266 (57%), Positives = 201/266 (75%), Gaps = 8/266 (3%)

Query: 24  ANDFTLPQPLDRYSAEDHATWATLYQRQCKLLPGRACDEFMEGLERLEVDADRVPDFNKL 83
           A D+T+PQ  D ++AE+H  W TL++RQ KLLPGRA   ++ GLE L++    +P+F +L
Sbjct: 16  AADWTIPQNWDAFTAEEHGVWDTLFERQIKLLPGRASKAYLHGLELLKLSDGGIPNFEEL 75

Query: 84  NQKLMAATGWKIVAVPGLIPDDVFFEHLANRRFPVTWWLREPHQLDYLQEPDVFHDLFGH 143
           +++L+  TGW++VAVPGL+PDDVFF+H+ANRRF    ++R P QLDYLQEPDVFHD+FGH
Sbjct: 76  SERLVKLTGWQVVAVPGLVPDDVFFDHMANRRFVAGNFIRRPDQLDYLQEPDVFHDVFGH 135

Query: 144 VPLLINPVFADYLEAYGKGGVKAKALGALPMLARLYWYTVEFGLINTPAGMRIYGAGILS 203
           VP+L +PVFADY+EAYG+GG +A  LGAL  L+RLYWYTVEFGLI     +RIYG+GI+S
Sbjct: 136 VPMLADPVFADYMEAYGRGGGRAMELGALKQLSRLYWYTVEFGLIEEAGDLRIYGSGIVS 195

Query: 204 SKSESIYCLDSASPNRVGFDLMRIMNTRYRIDTFQKTYFVIDSFKQLFDATA-PDFAPLY 262
           S +ES + L+S SPNR+GFDL R+M T YRID FQ+ YFVI SF+ L  AT   DFAPLY
Sbjct: 196 SSAESRFALESDSPNRIGFDLKRVMRTEYRIDDFQQNYFVIPSFETLLRATVETDFAPLY 255

Query: 263 LQLADAQPWGAGDVAPDDLVLNAGDR 288
            ++  AQP    D+   ++V  AGDR
Sbjct: 256 AEIL-AQP----DIPIAEIV--AGDR 274


Lambda     K      H
   0.323    0.140    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 310
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 297
Length of database: 288
Length adjustment: 26
Effective length of query: 271
Effective length of database: 262
Effective search space:    71002
Effective search space used:    71002
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate Ga0059261_1314 Ga0059261_1314 (Phenylalanine 4-hydroxylase (EC 1.14.16.1))
to HMM TIGR01267 (phhA: phenylalanine-4-hydroxylase (EC 1.14.16.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01267.hmm
# target sequence database:        /tmp/gapView.8056.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01267  [M=248]
Accession:   TIGR01267
Description: Phe4hydrox_mono: phenylalanine-4-hydroxylase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.3e-109  350.4   0.0   2.8e-109  350.1   0.0    1.0  1  lcl|FitnessBrowser__Korea:Ga0059261_1314  Ga0059261_1314 Phenylalanine 4-h


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Korea:Ga0059261_1314  Ga0059261_1314 Phenylalanine 4-hydroxylase (EC 1.14.16.1)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  350.1   0.0  2.8e-109  2.8e-109       1     240 [.      18     259 ..      18     264 .. 0.98

  Alignments for each domain:
  == domain 1  score: 350.1 bits;  conditional E-value: 2.8e-109
                                 TIGR01267   1 dftvaqdldryseeehavwatlidrqlkllegracdeyldGveklgldadripdleevneklraltGwk 69 
                                               d+t++q++d +++eeh vw tl++rq+kll+gra + yl+G+e l+l+   ip++ee++e+l +ltGw+
  lcl|FitnessBrowser__Korea:Ga0059261_1314  18 DWTIPQNWDAFTAEEHGVWDTLFERQIKLLPGRASKAYLHGLELLKLSDGGIPNFEELSERLVKLTGWQ 86 
                                               6799***************************************************************** PP

                                 TIGR01267  70 ivavpglipadvffehlanrrfpvttflrtpeeldylqepdvfhdlfGhvpllsnpvfadfleayGkkg 138
                                               +vavpgl+p+dvff+h+anrrf   +f+r p++ldylqepdvfhd+fGhvp+l++pvfad++eayG++g
  lcl|FitnessBrowser__Korea:Ga0059261_1314  87 VVAVPGLVPDDVFFDHMANRRFVAGNFIRRPDQLDYLQEPDVFHDVFGHVPMLADPVFADYMEAYGRGG 155
                                               ********************************************************************* PP

                                 TIGR01267 139 vkakalgaa.llarlywytvefGlvetaaglriyGaGilsskkelvyaleskeplrvafdllevmrtry 206
                                               ++a++lga  +l+rlywytvefGl+e+a++lriyG+Gi+ss  e+ +ales++p+r++fdl++vmrt+y
  lcl|FitnessBrowser__Korea:Ga0059261_1314 156 GRAMELGALkQLSRLYWYTVEFGLIEEAGDLRIYGSGIVSSSAESRFALESDSPNRIGFDLKRVMRTEY 224
                                               *******9889********************************************************** PP

                                 TIGR01267 207 ridklqkayfvlpslkrlfdaaqe.dfealvaeak 240
                                               rid +q+ yfv+ps++ l  a+ e df++l+ae  
  lcl|FitnessBrowser__Korea:Ga0059261_1314 225 RIDDFQQNYFVIPSFETLLRATVEtDFAPLYAEIL 259
                                               *****************99988777******9865 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (248 nodes)
Target sequences:                          1  (288 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 7.09
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis