Align argininosuccinate lyase (EC 4.3.2.1) (characterized)
to candidate BWI76_RS00855 BWI76_RS00855 argininosuccinate lyase
Query= ecocyc::ARGSUCCINLYA-MONOMER (457 letters) >FitnessBrowser__Koxy:BWI76_RS00855 Length = 457 Score = 868 bits (2242), Expect = 0.0 Identities = 438/456 (96%), Positives = 448/456 (98%) Query: 1 MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKALVTVGVLTAEEQAQLEE 60 MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKALVTVGVLTA+EQ QLEE Sbjct: 1 MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKALVTVGVLTADEQQQLEE 60 Query: 61 ALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKKLHTGRSRNDQVATDLKLW 120 ALN+LLEDVRA PQQILESDAEDIHSWVEGKLIDKVGQLGKKLHTGRSRNDQVATDLKLW Sbjct: 61 ALNILLEDVRANPQQILESDAEDIHSWVEGKLIDKVGQLGKKLHTGRSRNDQVATDLKLW 120 Query: 121 CKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRAQPVTFAHWCLAYVEMLARDE 180 CKDTV ELL+ANRQLQSALVETA+NNQDAVMPGYTHLQRAQPVTFAHWCLAYVEMLARDE Sbjct: 121 CKDTVVELLSANRQLQSALVETAENNQDAVMPGYTHLQRAQPVTFAHWCLAYVEMLARDE 180 Query: 181 SRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGFASATRNSLDSVSDRDHVLELLS 240 SRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGFASATRNSLDSVSDRDHVLELLS Sbjct: 181 SRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGFASATRNSLDSVSDRDHVLELLS 240 Query: 241 AAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSGSSLMPQKKNPDALELIRGKCGRVQ 300 A+IGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSGSSLMPQKKNPDALELIRGKCGRVQ Sbjct: 241 DASIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSGSSLMPQKKNPDALELIRGKCGRVQ 300 Query: 301 GALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDTWLDCLHMAALVLDGIQVKRPRCQEAA 360 GALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDTWLDCLHMAALVLDGIQVKRPRC+EAA Sbjct: 301 GALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDTWLDCLHMAALVLDGIQVKRPRCEEAA 360 Query: 361 QQGYANATELADYLVAKGVPFREAHHIVGEAVVEAIRQGKPLEDLPLSELQKFSQVIDED 420 QQGYANATELADYLVAKGVPFREAHHIVGEAVVEAI QGKPLEDLPL++LQKFS+VI ED Sbjct: 361 QQGYANATELADYLVAKGVPFREAHHIVGEAVVEAIAQGKPLEDLPLADLQKFSRVIAED 420 Query: 421 VYPILSLQSCLDKRAAKGGVSPQQVAQAIAFAQARL 456 VYPILSLQSCLDKRAAKGGVSPQQVAQAIA A+ RL Sbjct: 421 VYPILSLQSCLDKRAAKGGVSPQQVAQAIADAKQRL 456 Lambda K H 0.318 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 775 Number of extensions: 19 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 457 Length of database: 457 Length adjustment: 33 Effective length of query: 424 Effective length of database: 424 Effective search space: 179776 Effective search space used: 179776 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate BWI76_RS00855 BWI76_RS00855 (argininosuccinate lyase)
to HMM TIGR00838 (argH: argininosuccinate lyase (EC 4.3.2.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00838.hmm # target sequence database: /tmp/gapView.27091.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00838 [M=455] Accession: TIGR00838 Description: argH: argininosuccinate lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.5e-218 711.0 0.0 4e-218 710.9 0.0 1.0 1 lcl|FitnessBrowser__Koxy:BWI76_RS00855 BWI76_RS00855 argininosuccinate Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Koxy:BWI76_RS00855 BWI76_RS00855 argininosuccinate lyase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 710.9 0.0 4e-218 4e-218 1 454 [. 3 456 .. 3 457 .] 1.00 Alignments for each domain: == domain 1 score: 710.9 bits; conditional E-value: 4e-218 TIGR00838 1 lwggRlkkeldkavaefnaslsfDkelaeaDiegsiahtkaLakagilteeeakklieaLeelkeevkeg. 70 lwggR+++++d+++++fn+sl+fD++lae+Di gs+a++kaL+++g+lt++e+++l+eaL+ l e+v+++ lcl|FitnessBrowser__Koxy:BWI76_RS00855 3 LWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKALVTVGVLTADEQQQLEEALNILLEDVRANp 73 7********************************************************************98 PP TIGR00838 71 klelevdaeDiHlavErelidkvgedvgkklhtgrsRnDqvatdlrlylrdkvkelaealkdllkalvekA 141 ++ le+daeDiH++vE +lidkvg ++gkklhtgrsRnDqvatdl+l+++d+v el++a ++l++alve+A lcl|FitnessBrowser__Koxy:BWI76_RS00855 74 QQILESDAEDIHSWVEGKLIDKVG-QLGKKLHTGRSRNDQVATDLKLWCKDTVVELLSANRQLQSALVETA 143 9999********************.********************************************** PP TIGR00838 142 ekevetlmpgytHLqrAqPitlaHhllayaemlerDleRlldalkRvnksPlGsgAlagtsfeidrellae 212 e++++++mpgytHLqrAqP+t+aH++lay+eml+rD +Rl+dalkR+++sPlG+gAlagt++eidre+la lcl|FitnessBrowser__Koxy:BWI76_RS00855 144 ENNQDAVMPGYTHLQRAQPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAG 214 *********************************************************************** PP TIGR00838 213 lLgFdavvensldavsdRDfiiEllsaaallmvhlsrlaEelilfsseEfgfvelsdevssgssimPqKKn 283 +LgF+++++nsld+vsdRD+++Ells+a++ mvhlsr+aE+li+f++ E+gfvelsd+v+sgss+mPqKKn lcl|FitnessBrowser__Koxy:BWI76_RS00855 215 WLGFASATRNSLDSVSDRDHVLELLSDASIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSGSSLMPQKKN 285 *********************************************************************** PP TIGR00838 284 pDvaEliRgktgrviGnltglltilKalPlaYnkDlqEdkealfdalktveellevvtgllkelkvnkerl 354 pD++EliRgk+grv+G ltg++++lK+lPlaYnkD+qEdke+lfdal+t+ ++l++++++l+ ++v++ r+ lcl|FitnessBrowser__Koxy:BWI76_RS00855 286 PDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDTWLDCLHMAALVLDGIQVKRPRC 356 *********************************************************************** PP TIGR00838 355 eeaakknfalatdlAdylvrkGvPFReaheivGevvakaiekGkkleeltleelqklsekleedvlevldl 425 eeaa++++a+at+lAdylv+kGvPFReah+ivGe+v++ai++Gk le+l+l+ lqk+s + edv+ +l+l lcl|FitnessBrowser__Koxy:BWI76_RS00855 357 EEAAQQGYANATELADYLVAKGVPFREAHHIVGEAVVEAIAQGKPLEDLPLADLQKFSRVIAEDVYPILSL 427 *********************************************************************** PP TIGR00838 426 eeavekrdakGGtakeevekaieeakael 454 +++++kr+akGG+++++v++ai+ ak++l lcl|FitnessBrowser__Koxy:BWI76_RS00855 428 QSCLDKRAAKGGVSPQQVAQAIADAKQRL 456 **************************986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (455 nodes) Target sequences: 1 (457 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 12.07 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory