Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate BWI76_RS21480 BWI76_RS21480 phospho-2-dehydro-3-deoxyheptonate aldolase
Query= BRENDA::P00888 (356 letters) >FitnessBrowser__Koxy:BWI76_RS21480 Length = 356 Score = 655 bits (1691), Expect = 0.0 Identities = 325/356 (91%), Positives = 341/356 (95%) Query: 1 MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKSISDIIAGRDPRLLVVCG 60 MQKDALNNVHITDEQVL+TP+QLKA FPLS++QEAQIA SR++ISDIIAGRDPRLLVVCG Sbjct: 1 MQKDALNNVHITDEQVLITPDQLKAEFPLSVEQEAQIAHSRQTISDIIAGRDPRLLVVCG 60 Query: 61 PCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDV 120 PCSIHDPETA+EYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDV Sbjct: 61 PCSIHDPETAIEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDV 120 Query: 121 EAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGL 180 E GL+IAR LL+ELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGL Sbjct: 121 EGGLKIARHLLVELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGL 180 Query: 181 SMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVILRGGKAP 240 SMPVGFKNGTDGSLATAINAMRAAA PHRFVGINQAGQV LLQTQGNP+GHVILRGGKAP Sbjct: 181 SMPVGFKNGTDGSLATAINAMRAAAMPHRFVGINQAGQVCLLQTQGNPNGHVILRGGKAP 240 Query: 241 NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGL 300 NY P DVA+CEKEM QAGL+PSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGL Sbjct: 241 NYGPEDVAKCEKEMTQAGLKPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGL 300 Query: 301 MIESNIHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA 356 MIESNIHEGNQSSEQPR +MKYGVSVTDACISWE TDALLRE+ +DL G L AR+A Sbjct: 301 MIESNIHEGNQSSEQPRCDMKYGVSVTDACISWETTDALLRELDKDLRGHLAARLA 356 Lambda K H 0.316 0.131 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 593 Number of extensions: 10 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 356 Length of database: 356 Length adjustment: 29 Effective length of query: 327 Effective length of database: 327 Effective search space: 106929 Effective search space used: 106929 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 49 (23.5 bits)
Align candidate BWI76_RS21480 BWI76_RS21480 (phospho-2-dehydro-3-deoxyheptonate aldolase)
to HMM TIGR00034 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00034.hmm # target sequence database: /tmp/gapView.22727.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00034 [M=342] Accession: TIGR00034 Description: aroFGH: 3-deoxy-7-phosphoheptulonate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.8e-176 569.6 0.0 1e-175 569.4 0.0 1.0 1 lcl|FitnessBrowser__Koxy:BWI76_RS21480 BWI76_RS21480 phospho-2-dehydro- Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Koxy:BWI76_RS21480 BWI76_RS21480 phospho-2-dehydro-3-deoxyheptonate aldolase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 569.4 0.0 1e-175 1e-175 5 340 .. 8 349 .. 4 351 .. 0.96 Alignments for each domain: == domain 1 score: 569.4 bits; conditional E-value: 1e-175 TIGR00034 5 ivki...delltPeelkakfpltekaaekvaksrkeiadilaGkddrllvviGPcsihdpeaaleyakrlk 72 +v+i + l+tP++lka+fpl+ ++++++a+sr++i+di+aG+d+rllvv+GPcsihdpe+a+eya+r+k lcl|FitnessBrowser__Koxy:BWI76_RS21480 8 NVHItdeQVLITPDQLKAEFPLSVEQEAQIAHSRQTISDIIAGRDPRLLVVCGPCSIHDPETAIEYARRFK 78 44444315577************************************************************ PP TIGR00034 73 klaeklkddleivmrvyfekPrttvGWkGlindPdlnesfdvnkGlriarkllldlvelglplatelldti 143 +la++++d+l++vmrvyfekPrttvGWkGlindP++++sfdv+ Gl+iar+ll++lv++glplate+ld++ lcl|FitnessBrowser__Koxy:BWI76_RS21480 79 ALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEGGLKIARHLLVELVNMGLPLATEALDPN 149 *********************************************************************** PP TIGR00034 144 spqyladllswgaiGarttesqvhrelasglslpvgfkngtdGslkvaidairaaaaehlflsvtkaGqva 214 spqyl+dl+sw+aiGarttesq+hre+asgls+pvgfkngtdGsl++ai+a+raaa +h+f+++++aGqv+ lcl|FitnessBrowser__Koxy:BWI76_RS21480 150 SPQYLGDLFSWSAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAMPHRFVGINQAGQVC 220 *********************************************************************** PP TIGR00034 215 ivetkGnedghiilrGGkkpnydaedvaevkeelekaglkeelmidfshgnsnkdykrqlevaesvveqia 285 +++t+Gn++gh+ilrGGk+pny +edva++++e+++aglk++lm+d+shgnsnkdy+rq++vaesvv+qi+ lcl|FitnessBrowser__Koxy:BWI76_RS21480 221 LLQTQGNPNGHVILRGGKAPNYGPEDVAKCEKEMTQAGLKPSLMVDCSHGNSNKDYRRQPAVAESVVAQIK 291 *********************************************************************** PP TIGR00034 286 eGekaiiGvmiesnleeGnqslke...elkyGksvtdacigwedteallrklaeavke 340 +G+++iiG+miesn++eGnqs+++ ++kyG+svtdaci+we+t+allr+l ++++ lcl|FitnessBrowser__Koxy:BWI76_RS21480 292 DGNRSIIGLMIESNIHEGNQSSEQprcDMKYGVSVTDACISWETTDALLRELDKDLRG 349 *********************96522269************************99986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (342 nodes) Target sequences: 1 (356 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 10.32 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory