Alignments for a candidate for cysK in Klebsiella michiganensis M5al

GapMind for Amino acid biosynthesis

Alignments for a candidate for cysK in Klebsiella michiganensis M5al

Align cysteine synthase (EC 2.5.1.47) (characterized)
to candidate BWI76_RS20640 BWI76_RS20640 cysteine synthase A

Query= BRENDA::P0ABK5
         (323 letters)



>FitnessBrowser__Koxy:BWI76_RS20640
          Length = 323

 Score =  611 bits (1575), Expect = e-180
 Identities = 313/323 (96%), Positives = 319/323 (98%)

Query: 1   MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGVLK 60
           MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGVLK
Sbjct: 1   MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGVLK 60

Query: 61  PGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGM 120
           PGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGM
Sbjct: 61  PGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGM 120

Query: 121 KGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPEIWEDTDGQVDVFIAGVGTGG 180
           KGAIQKAEEIVASNPEK+LLLQQFSNPANPEIHEKTTGPEIWEDTDGQVDVFI+GVGTGG
Sbjct: 121 KGAIQKAEEIVASNPEKFLLLQQFSNPANPEIHEKTTGPEIWEDTDGQVDVFISGVGTGG 180

Query: 181 TLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGEEIKPGPHKIQGIGAGFIPANLD 240
           TLTGVSRYIKGTKGK DLI+VAVEPTDSPVIAQALAG E+KPGPHKIQGIGAGFIP NLD
Sbjct: 181 TLTGVSRYIKGTKGKKDLITVAVEPTDSPVIAQALAGVELKPGPHKIQGIGAGFIPGNLD 240

Query: 241 LKLVDKVIGITNEEAISTARRLMEEEGILAGISSGAAVAAALKLQEDESFTNKNIVVILP 300
           LKLVDKV+ ITNEEAISTARRLMEEEGILAGISSGAAVAAALKLQ+DESFTNKNIVVILP
Sbjct: 241 LKLVDKVVAITNEEAISTARRLMEEEGILAGISSGAAVAAALKLQQDESFTNKNIVVILP 300

Query: 301 SSGERYLSTALFADLFTEKELQQ 323
           SSGERYLSTALFADLFTEKELQQ
Sbjct: 301 SSGERYLSTALFADLFTEKELQQ 323


Lambda     K      H
   0.313    0.133    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 491
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 323
Length of database: 323
Length adjustment: 28
Effective length of query: 295
Effective length of database: 295
Effective search space:    87025
Effective search space used:    87025
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate BWI76_RS20640 BWI76_RS20640 (cysteine synthase A)
to HMM TIGR01139 (cysK: cysteine synthase A (EC 2.5.1.47))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01139.hmm
# target sequence database:        /tmp/gapView.21982.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01139  [M=298]
Accession:   TIGR01139
Description: cysK: cysteine synthase A
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                               -----------
   8.3e-150  484.0   0.9   9.5e-150  483.8   0.9    1.0  1  lcl|FitnessBrowser__Koxy:BWI76_RS20640  BWI76_RS20640 cysteine synthase 


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Koxy:BWI76_RS20640  BWI76_RS20640 cysteine synthase A
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  483.8   0.9  9.5e-150  9.5e-150       2     298 .]       9     312 ..       8     312 .. 0.98

  Alignments for each domain:
  == domain 1  score: 483.8 bits;  conditional E-value: 9.5e-150
                               TIGR01139   2 seliGntPlvrLnlaeeakaevlvkleslnPsssvkdrialamiedaekegllkkgktiveatsGntGial 72 
                                             s +iG+tPlvrLn +++ ++++l+k+es+nPs+svk+ri+++mi+daek+g+lk+g ++ve+tsGntGial
  lcl|FitnessBrowser__Koxy:BWI76_RS20640   9 SLTIGHTPLVRLN-RIG-NGRILAKVESRNPSFSVKCRIGANMIWDAEKRGVLKPGVELVEPTSGNTGIAL 77 
                                             569**********.666.89*************************************************** PP

                               TIGR01139  73 amvaaargykliltmpetmslerrkllkayGaelvLtdgaegmkgaiekaeelveetpnkylllkqfenpa 143
                                             a+vaaargykl+ltmpetms+errkllka+Ga+lvLt+ga+gmkgai+kaee+v+++p+k+lll+qf+npa
  lcl|FitnessBrowser__Koxy:BWI76_RS20640  78 AYVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKFLLLQQFSNPA 148
                                             *********************************************************************** PP

                               TIGR01139 144 npeihrkttapeilkdldgkldafvagvGtGGtitGvgevlkekkp..dikvvavePaespvlsgg..... 207
                                             npeih+ktt+pei++d+dg++d+f++gvGtGGt+tGv++++k +k+  d+  vaveP++spv++++     
  lcl|FitnessBrowser__Koxy:BWI76_RS20640 149 NPEIHEKTTGPEIWEDTDGQVDVFISGVGTGGTLTGVSRYIKGTKGkkDLITVAVEPTDSPVIAQAlagve 219
                                             ******************************************9987679****************999*** PP

                               TIGR01139 208 .kpgphkiqGigagfiPkvLdkevidevikvsdeeaietarrlakeeGilvGissGaavaaalkvakkle. 276
                                              kpgphkiqGigagfiP +Ld +++d+v+++++eeai+tarrl++eeGil+GissGaavaaalk++++++ 
  lcl|FitnessBrowser__Koxy:BWI76_RS20640 220 lKPGPHKIQGIGAGFIPGNLDLKLVDKVVAITNEEAISTARRLMEEEGILAGISSGAAVAAALKLQQDESf 290
                                             *********************************************************************** PP

                               TIGR01139 277 kdkkivvilpdtgerYlstaLf 298
                                             ++k+ivvilp++gerYlstaLf
  lcl|FitnessBrowser__Koxy:BWI76_RS20640 291 TNKNIVVILPSSGERYLSTALF 312
                                             *********************9 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (298 nodes)
Target sequences:                          1  (323 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 11.76
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis