Align glutamine synthetase (EC 6.3.1.2) (characterized)
to candidate BWI76_RS10710 BWI76_RS10710 gamma-glutamylputrescine synthetase
Query= BRENDA::O33342 (457 letters) >FitnessBrowser__Koxy:BWI76_RS10710 Length = 472 Score = 167 bits (423), Expect = 7e-46 Identities = 139/436 (31%), Positives = 204/436 (46%), Gaps = 23/436 (5%) Query: 26 VIVAFTDMQGRLAGKRI--SGRHFVDDIATRGVECCSYLLAVDVDLNTVPGYAMASWDTG 83 V V TD+ G GKRI SG ++ +G + + A+D+ L V A + G Sbjct: 40 VDVLLTDLNGCFRGKRIPVSGLSKLE----KGCYFPASVFAMDI-LGNVVEEAGLGQEMG 94 Query: 84 YGDMVMTPDLSTLRLIPWLPG-TALVIADLVWADGSEVAVSPRSILRRQLDRLKARGLVA 142 D P L TL P V+ +V DG+ V PR++L R +L+ RGL Sbjct: 95 EPDRTCVPVLGTLTPSAADPEYIGQVLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFP 154 Query: 143 DVATELEFIVFDQPYRQAWASGYR------GLTPASDYNIDYAILASSRMEPLLRDIRLG 196 VA ELEF + D R+ A GY G + + Y++ + +L +I Sbjct: 155 VVAVELEFYLLD---RKRDAEGYLQPPCAPGTGDRNTQSQVYSVDNLNHFADVLNEIDEI 211 Query: 197 MAGAGLRFEAVKGECNMGQQEIGFRY-DEALVTCDNHAIYKNGAKEIADQHGKSLTFMAK 255 + + E + GQ EI + D L CD+ K + +A++H TFMAK Sbjct: 212 AQLQLIPADGAVAEASPGQFEINLHHTDNVLDACDDALALKRLVRLMAEKHKMHATFMAK 271 Query: 256 -YDEREGNSCHIHVSLRGTDGSAVFADSNGPHGMSSMFRSFVAGQLATLREFTLCYAPTI 314 Y+E G+ HIH+S++ G V AD++G S+M + +AG + + AP + Sbjct: 272 PYEEHAGSGMHIHISMQNNKGENVLADADGED--SAMLKRALAGMIDLMPASMALLAPNV 329 Query: 315 NSYKRFADSSFAPTALAWGLDNRTCALRV-VGHGQNIRVECRVPGGDVNQYLAVAALIAG 373 NSY+RF + PT +WG +NRT ALR+ G N RVE RV G D N YL +AA+ AG Sbjct: 330 NSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAG 389 Query: 374 GLYGIERGLQLPEPCVGNAYQGADVERLPVTLADAAVLFEDSALVREAFGEDVVAHYLNN 433 L+G++ L L E GN + + P+ +DA F + +RE GE Y Sbjct: 390 ILHGLDNPLPLQEEVEGNGLEQEGLP-FPIRQSDALWEFMQNDHLRERLGERFCHVYHAC 448 Query: 434 ARVELAAFNAAVTDWE 449 EL F +T+ E Sbjct: 449 KNDELLQFERLITETE 464 Lambda K H 0.321 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 529 Number of extensions: 30 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 457 Length of database: 472 Length adjustment: 33 Effective length of query: 424 Effective length of database: 439 Effective search space: 186136 Effective search space used: 186136 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory