Align acetolactate synthase (subunit 2/2) (EC 2.2.1.6) (characterized)
to candidate BWI76_RS06990 BWI76_RS06990 glyoxylate carboligase
Query= BRENDA::P9WG41 (618 letters) >FitnessBrowser__Koxy:BWI76_RS06990 Length = 593 Score = 344 bits (882), Expect = 7e-99 Identities = 203/558 (36%), Positives = 293/558 (52%), Gaps = 14/558 (2%) Query: 35 LQQLTGAQAVIRSLEELGVDVIFGIPGGAVLPVYDPLFDSKKLRHVLVRHEQGAGHAASG 94 + ++ A + LE+ G+ FG+PG A+ P Y + +RH+L RH +GA H A G Sbjct: 1 MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG 60 Query: 95 YAHVT-GRVGVCMATSGPGATNLVTPLADAQMDSIPVVAITGQVGRGLIGTDAFQEADIS 153 Y T G +GVC+ TSGP T+++T L A DS+P++ ITGQ R + + FQ DI Sbjct: 61 YTRATAGNIGVCLGTSGPAGTDMITALYSASADSVPILCITGQAPRARLHKEDFQAVDIE 120 Query: 154 GITMPITKHNFLVRSGDDIPRVLAEAFHIAASGRPGAVLVDIPKDVLQGQCTFSWPPRME 213 I P+TK VR +P VL +AFH+ SGRPG VL+D+P DV + F Sbjct: 121 SIAKPVTKMAVTVREAALVPGVLQQAFHLMRSGRPGPVLIDLPYDVQVAEIEFDPELYAP 180 Query: 214 LPGYKP-NTKPHSRQVREAAKLIAAARKPVLYVGGGVIRGEATEQLRELAELTGIPVVTT 272 LP YKP T+P Q+ A ++ A +PV+ GGGVI +A L++ AE+T +PV+ T Sbjct: 181 LPAYKPVATRP---QIERALAMLLMAERPVIVAGGGVINADAAALLQQFAEITHVPVIPT 237 Query: 273 LMARGAFPDSHRQNLGMPGMHGTVA-AVAALQRSDLLIALGTRFDDRVTGKLDSFAPEAK 331 LM G PD H GM G+ A L SDL+ +G RF +R TG ++ + K Sbjct: 238 LMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDLVFGIGNRFANRHTGSVEKYTAGRK 297 Query: 332 VIHADIDPAEIGKNRHADVPIVGDVKAVITELIAMLRHHHIPGTIEMADWWAYLNGVRKT 391 +IH DI+P +IG+ D+ IV D KA +T+L+ + + G + + W RK Sbjct: 298 IIHIDIEPTQIGRVLCPDLGIVSDAKAALTQLVDIAQQMQKEGRLPRRERWVAECQERKR 357 Query: 392 YPLSYGPQSDGSLSPEYVIEKLGEIAGPDAVFVAGVGQHQMWAAQFIRYEKPRSWLNSGG 451 L + + P+ V E++ + G D +V +G Q+ AAQ + K R W+N G Sbjct: 358 TLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQ 417 Query: 452 LGTMGFAIPAAMGAKIALPGTEVWAIDGDGCFQMTNQELATCAVEGIPVKVALINNGNLG 511 G +G+ +PAA+G A P V AI GD FQ +ELA A IP L+NN LG Sbjct: 418 AGPLGWTLPAALGVCAADPERNVVAISGDFDFQFLIEELAVGAQFNIPYVHVLVNNAYLG 477 Query: 512 MVRQWQSLFYAERYSQTDLAT-HSHRI----PDFVKLAEALGCVGLRCEREEDVVDVINQ 566 ++RQ Q F + Q +S + D VK+AE LGC +R + ED+ Q Sbjct: 478 LIRQSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQ 537 Query: 567 ARAIND---CPVVIDFIV 581 A+A PVV++ I+ Sbjct: 538 AKAWMKEYRVPVVVEVIL 555 Lambda K H 0.319 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 917 Number of extensions: 54 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 618 Length of database: 593 Length adjustment: 37 Effective length of query: 581 Effective length of database: 556 Effective search space: 323036 Effective search space used: 323036 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory