Alignments for a candidate for ilvI in Klebsiella michiganensis M5al

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Klebsiella michiganensis M5al

Align Acetolactate synthase isozyme 2 large subunit; AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit; EC 2.2.1.6 (characterized)
to candidate BWI76_RS27815 BWI76_RS27815 acetolactate synthase catalytic subunit

Query= SwissProt::P0DP90
         (548 letters)



>FitnessBrowser__Koxy:BWI76_RS27815
          Length = 562

 Score =  505 bits (1301), Expect = e-147
 Identities = 269/552 (48%), Positives = 359/552 (65%), Gaps = 16/552 (2%)

Query: 3   GAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGG-VEHLLCRHEQGAAMAAIGYARAT 61
           GAQ VVH L  QG+ TV G PGG+I+P+YDAL     + H+L RHEQGA   A G AR  
Sbjct: 15  GAQLVVHLLERQGITTVSGIPGGSILPIYDALSQSTQIRHILARHEQGAGFIAQGMARTE 74

Query: 62  GKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLAC 121
           GK  VC+A SGPGATNLIT +ADA LDSIP+V ITGQV A  IGTDAFQEVD  G+S+  
Sbjct: 75  GKPAVCMACSGPGATNLITAIADARLDSIPLVCITGQVPASMIGTDAFQEVDTYGISIPI 134

Query: 122 TKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKDIQLASGDL----EPWFTTVEN 177
           TKH++LV+++ ELP++M++AF +A SGRPGPV +DIPKD+Q A+ +L    EP       
Sbjct: 135 TKHNYLVRNIAELPQVMSDAFRIAQSGRPGPVWIDIPKDVQAATIELDALPEPGARMAAP 194

Query: 178 EVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLAATKMPATCTLKGLGAV 237
           E  F  A V +A  M+  AQ+P+LY+GGGV    A   +R+      +P T TL  LG +
Sbjct: 195 E--FDSASVREAAAMINAAQRPVLYLGGGV--INAPEHIRQLAEKANLPTTQTLMALGML 250

Query: 238 EADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDP 297
              +P  LGMLGMHG ++ NF +QE DLL+ +GARFDDR  GK   F P+A +IH+DID 
Sbjct: 251 PKAHPLSLGMLGMHGARSTNFILQEADLLVVLGARFDDRAIGKTEQFCPNAKIIHVDIDR 310

Query: 298 AEMNKLRQAHVALQGD----LNALLPALQ-QPLNQYDWQQHCAQLRDEHSWRYDHPGDAI 352
           +E+ K++QAHVA+QGD    L  L+P ++ QP +   W Q  A L+ E         D +
Sbjct: 311 SELGKIKQAHVAIQGDVGEVLEQLIPQVEAQPRSA--WLQLVADLQREFPCTIPQEQDPL 368

Query: 353 YAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGLGTMGFGLPAAVG 412
               L+  ++     + +VTTDVGQHQMW AQ     RP  ++TS GLGTMGFGLPAAVG
Sbjct: 369 SHYGLINAVAACVDDEAIVTTDVGQHQMWVAQAYPLNRPRQWLTSGGLGTMGFGLPAAVG 428

Query: 413 AQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQER 472
           A +A P   V+C SGDGS MMN+QE+ T    QL +KI+L++N+ LG+V Q Q LF+++ 
Sbjct: 429 AALANPQRKVICFSGDGSLMMNIQEMATAAENQLDVKIILMNNEALGLVYQQQSLFYKQG 488

Query: 473 YSETTLTDNPDFLMLASAFGIHGQHITRKDQVEAALDTMLNSDGPYLLHVSIDELENVWP 532
               T     +F+ +AS FG+    +  +   +AAL  +++  GP L+HV ID  E V+P
Sbjct: 489 VFAATYPGMVNFMQIASGFGLQTCDLNNEADPQAALQAIIDRPGPALIHVRIDAEEKVYP 548

Query: 533 LVPPGASNSEML 544
           +VPPGA+N+EM+
Sbjct: 549 MVPPGAANTEMV 560


Lambda     K      H
   0.320    0.135    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 828
Number of extensions: 32
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 548
Length of database: 562
Length adjustment: 36
Effective length of query: 512
Effective length of database: 526
Effective search space:   269312
Effective search space used:   269312
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

Align candidate BWI76_RS27815 BWI76_RS27815 (acetolactate synthase catalytic subunit)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00118.hmm
# target sequence database:        /tmp/gapView.7872.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00118  [M=557]
Accession:   TIGR00118
Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                               -----------
   1.4e-205  669.8   0.8   1.6e-205  669.6   0.8    1.0  1  lcl|FitnessBrowser__Koxy:BWI76_RS27815  BWI76_RS27815 acetolactate synth


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Koxy:BWI76_RS27815  BWI76_RS27815 acetolactate synthase catalytic subunit
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  669.6   0.8  1.6e-205  1.6e-205       2     555 ..      14     561 ..      13     562 .] 0.97

  Alignments for each domain:
  == domain 1  score: 669.6 bits;  conditional E-value: 1.6e-205
                               TIGR00118   2 kgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvlats 71 
                                             +ga+++v+ l+++g+ tv G+PGG++lpiydal  + +++hil+rheq+a  +a+G+ar+ Gk+ v++a s
  lcl|FitnessBrowser__Koxy:BWI76_RS27815  14 TGAQLVVHLLERQGITTVSGIPGGSILPIYDALSqSTQIRHILARHEQGAGFIAQGMARTEGKPAVCMACS 84 
                                             79*******************************97889********************************* PP

                               TIGR00118  72 GPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpeilkeaf 142
                                             GPGatnl+t+ia+a lds+Plv +tGqv++s+iG+dafqe+d  Gi++p+tkh++lv++ ++lp+++ +af
  lcl|FitnessBrowser__Koxy:BWI76_RS27815  85 GPGATNLITAIADARLDSIPLVCITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRNIAELPQVMSDAF 155
                                             *********************************************************************** PP

                               TIGR00118 143 eiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvllvGgGvii 213
                                              ia++GrPGPv +d+Pkdv++a+iel+   +   +  +p+   + + +++a+ +i++a++Pvl+ GgGvi 
  lcl|FitnessBrowser__Koxy:BWI76_RS27815 156 RIAQSGRPGPVWIDIPKDVQAATIELDALPEPGARMAAPEF--DSASVREAAAMINAAQRPVLYLGGGVI- 223
                                             *************************9998776666666655..56789*********************9. PP

                               TIGR00118 214 aeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGarfddrvtgn 284
                                              +a+e++++lae++++p t+tl+ lG +p+ hpl+lgmlGmhG++ +n+ ++eadll+ +Garfddr  g+
  lcl|FitnessBrowser__Koxy:BWI76_RS27815 224 -NAPEHIRQLAEKANLPTTQTLMALGMLPKAHPLSLGMLGMHGARSTNFILQEADLLVVLGARFDDRAIGK 293
                                             .57999***************************************************************** PP

                               TIGR00118 285 lakfapeakiihididPaeigknvkvdipivGdakkvleellkklkeeekkekeWlekieewkkeyilkld 355
                                              ++f+p+akiih+did +e+gk+ +++++i Gd+ +vle+l+ +++++ ++   Wl+ ++++++e + ++ 
  lcl|FitnessBrowser__Koxy:BWI76_RS27815 294 TEQFCPNAKIIHVDIDRSELGKIKQAHVAIQGDVGEVLEQLIPQVEAQPRSA--WLQLVADLQREFPCTIP 362
                                             **********************************************999998..***************** PP

                               TIGR00118 356 eeeesikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfitsgGlGtmGfGlPaalGakvak 426
                                             +e++++    +i+++   ++deaivttdvGqhqmw+aq y+ ++pr+++tsgGlGtmGfGlPaa+Ga +a+
  lcl|FitnessBrowser__Koxy:BWI76_RS27815 363 QEQDPLSHYGLINAVAACVDDEAIVTTDVGQHQMWVAQAYPLNRPRQWLTSGGLGTMGFGLPAAVGAALAN 433
                                             *********************************************************************** PP

                               TIGR00118 427 peetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWqelfyeerysetklaselpdfvkl 497
                                             p+ +v++ +Gdgs++mn+qe++t++e +++vki+++nne lG+v+q q+lfy++   +++   ++ +f+++
  lcl|FitnessBrowser__Koxy:BWI76_RS27815 434 PQRKVICFSGDGSLMMNIQEMATAAENQLDVKIILMNNEALGLVYQQQSLFYKQGVFAATYP-GMVNFMQI 503
                                             *****************************************************999888877.59****** PP

                               TIGR00118 498 aeayGvkgiriekpeeleeklkealeskepvlldvevdkeeevlPmvapGagldelve 555
                                             a ++G+++  ++++++ +++l+ ++   +p+l+ v++d ee+v+Pmv+pGa+++e+v+
  lcl|FitnessBrowser__Koxy:BWI76_RS27815 504 ASGFGLQTCDLNNEADPQAALQAIIDRPGPALIHVRIDAEEKVYPMVPPGAANTEMVG 561
                                             ********************************************************96 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (557 nodes)
Target sequences:                          1  (562 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02
# Mc/sec: 12.52
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis