Alignments for a candidate for asd in Klebsiella michiganensis M5al

GapMind for Amino acid biosynthesis

Alignments for a candidate for asd in Klebsiella michiganensis M5al

Align aspartate-semialdehyde dehydrogenase (characterized)
to candidate BWI76_RS26220 BWI76_RS26220 aspartate-semialdehyde dehydrogenase

Query= CharProtDB::CH_024631
         (367 letters)



>FitnessBrowser__Koxy:BWI76_RS26220
          Length = 368

 Score =  687 bits (1772), Expect = 0.0
 Identities = 343/368 (93%), Positives = 357/368 (97%), Gaps = 1/368 (0%)

Query: 1   MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAAPSFGGTTG-TLQDAFD 59
           MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQ APSFGG+TG TLQDAFD
Sbjct: 1   MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQPAPSFGGSTGGTLQDAFD 60

Query: 60  LEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLRMKDDAIIILDPVNQDVI 119
           LEALKALDIIVTCQGGDYTN+IYPKLRESGWQGYWIDAASSLRMKDDAIIILDPVNQDVI
Sbjct: 61  LEALKALDIIVTCQGGDYTNDIYPKLRESGWQGYWIDAASSLRMKDDAIIILDPVNQDVI 120

Query: 120 TDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVATYQAASGGGARHMRELLTQM 179
           T GLNNG++TFVGGNCTVSLMLMSLGGLFA DLV+WVSVATYQAASGGGARHMRELLTQM
Sbjct: 121 TAGLNNGVKTFVGGNCTVSLMLMSLGGLFAQDLVEWVSVATYQAASGGGARHMRELLTQM 180

Query: 180 GHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNFGVPLAGSLIPWIDKQLDNGQS 239
           G L+ HVA ELA P+SAIL+IERKVT+LTRSGEL VDNFGVPLAGSLIPWIDKQLDNGQS
Sbjct: 181 GQLHSHVAAELADPASAILEIERKVTSLTRSGELAVDNFGVPLAGSLIPWIDKQLDNGQS 240

Query: 240 REEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQAFTIKLKKDVSIPTVEELLAAHN 299
           REEWKGQAETNKIL T+S IPVDGLCVRVGALRCHSQAFTIKLKK+VSIPTVEELLAAHN
Sbjct: 241 REEWKGQAETNKILATASPIPVDGLCVRVGALRCHSQAFTIKLKKEVSIPTVEELLAAHN 300

Query: 300 PWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRKLNMGPEFLSAFTVGDQLLWGAAEPL 359
           PWAKVVPNDR+ITMRELTPAAVTGTLTTPVGRLRKLNMGPE+LSAFTVGDQLLWGAAEPL
Sbjct: 301 PWAKVVPNDRDITMRELTPAAVTGTLTTPVGRLRKLNMGPEYLSAFTVGDQLLWGAAEPL 360

Query: 360 RRMLRQLA 367
           RRML+QLA
Sbjct: 361 RRMLKQLA 368


Lambda     K      H
   0.319    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 582
Number of extensions: 14
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 367
Length of database: 368
Length adjustment: 30
Effective length of query: 337
Effective length of database: 338
Effective search space:   113906
Effective search space used:   113906
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate BWI76_RS26220 BWI76_RS26220 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR01745 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01745.hmm
# target sequence database:        /tmp/gapView.16052.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01745  [M=366]
Accession:   TIGR01745
Description: asd_gamma: aspartate-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                               -----------
   4.9e-222  722.4   0.0   5.5e-222  722.2   0.0    1.0  1  lcl|FitnessBrowser__Koxy:BWI76_RS26220  BWI76_RS26220 aspartate-semialde


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Koxy:BWI76_RS26220  BWI76_RS26220 aspartate-semialdehyde dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  722.2   0.0  5.5e-222  5.5e-222       1     365 [.       2     367 ..       2     368 .] 0.99

  Alignments for each domain:
  == domain 1  score: 722.2 bits;  conditional E-value: 5.5e-222
                               TIGR01745   1 kkvglvgwrgmvgsvllkrmqeekdfdaikpvffstsqlgqkapslakisa.iledaydidalkeldiiit 70 
                                             k+vg++gwrgmvgsvl++rm+ee+dfdai+pvffstsqlgq aps+++ ++ +l+da+d++alk+ldii+t
  lcl|FitnessBrowser__Koxy:BWI76_RS26220   2 KNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQPAPSFGGSTGgTLQDAFDLEALKALDIIVT 72 
                                             69********************************************998877******************* PP

                               TIGR01745  71 cqggdytkeiypklrkagwkgywidaasslrmkddaviildpvnldvikdavnkgirtfvggnctvslllm 141
                                             cqggdyt++iypklr++gw+gywidaasslrmkdda+iildpvn+dvi+ ++n+g++tfvggnctvsl+lm
  lcl|FitnessBrowser__Koxy:BWI76_RS26220  73 CQGGDYTNDIYPKLRESGWQGYWIDAASSLRMKDDAIIILDPVNQDVITAGLNNGVKTFVGGNCTVSLMLM 143
                                             *********************************************************************** PP

                               TIGR01745 142 slgglfrdelvewvsvatyqaasgggarhmrellkqmgvlykeveeelakpssaileierkvtklsrseel 212
                                             slgglf+++lvewvsvatyqaasgggarhmrell+qmg l+ +v+ ela p+saileierkvt l+rs+el
  lcl|FitnessBrowser__Koxy:BWI76_RS26220 144 SLGGLFAQDLVEWVSVATYQAASGGGARHMRELLTQMGQLHSHVAAELADPASAILEIERKVTSLTRSGEL 214
                                             *********************************************************************** PP

                               TIGR01745 213 pvenfsvplagslipwidkqldngqsreewkgqaetnkilgtkdtilvdglcvrigalrchsqaltiklkk 283
                                              v+nf+vplagslipwidkqldngqsreewkgqaetnkil+t++ i+vdglcvr+galrchsqa+tiklkk
  lcl|FitnessBrowser__Koxy:BWI76_RS26220 215 AVDNFGVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILATASPIPVDGLCVRVGALRCHSQAFTIKLKK 285
                                             *********************************************************************** PP

                               TIGR01745 284 dvsleeieeiirahnkwvkvvpnereitlreltpaavtgtldipvgrlrklnmgkeylsaftvgdqllwga 354
                                             +vs++++ee+++ahn+w+kvvpn+r+it+reltpaavtgtl++pvgrlrklnmg+eylsaftvgdqllwga
  lcl|FitnessBrowser__Koxy:BWI76_RS26220 286 EVSIPTVEELLAAHNPWAKVVPNDRDITMRELTPAAVTGTLTTPVGRLRKLNMGPEYLSAFTVGDQLLWGA 356
                                             *********************************************************************** PP

                               TIGR01745 355 aeplrrmlril 365
                                             aeplrrml++l
  lcl|FitnessBrowser__Koxy:BWI76_RS26220 357 AEPLRRMLKQL 367
                                             *********98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (366 nodes)
Target sequences:                          1  (368 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 7.67
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis