Alignments for a candidate for ilvE in Klebsiella michiganensis M5al

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvE in Klebsiella michiganensis M5al

Align valine-pyruvate transaminase (EC 2.6.1.66) (characterized)
to candidate BWI76_RS27065 BWI76_RS27065 valine--pyruvate transaminase

Query= BRENDA::P09053
         (417 letters)



>FitnessBrowser__Koxy:BWI76_RS27065
          Length = 419

 Score =  784 bits (2025), Expect = 0.0
 Identities = 376/415 (90%), Positives = 397/415 (95%)

Query: 1   MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIPEMQDYFQTLLTDMLES 60
           MTFSLFGDKFTRHSGIT LMEDLNDGLRTPGAIMLGGGNPAQIPEM DYF +LL DML S
Sbjct: 1   MTFSLFGDKFTRHSGITRLMEDLNDGLRTPGAIMLGGGNPAQIPEMNDYFHSLLADMLNS 60

Query: 61  GKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIALTNGSQSAFFYLFNLFAGR 120
           GKA DALCNYDGPQGK+ELLTLLA MLR++LGW+IEPQNIALTNGSQSAFFYLFNLFAGR
Sbjct: 61  GKALDALCNYDGPQGKSELLTLLAQMLRDELGWEIEPQNIALTNGSQSAFFYLFNLFAGR 120

Query: 121 RADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELLPEGQFKYHVDFEHLHIGEET 180
           RADG  +KVLFPL PEYIGYAD+GLEEDLFV+ RPNIELLPEGQFKYHVDFEHL + +ET
Sbjct: 121 RADGTTRKVLFPLTPEYIGYADSGLEEDLFVATRPNIELLPEGQFKYHVDFEHLQVTDET 180

Query: 181 GMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVIDNAYGVPFPGIIFSEARPLWNPN 240
           GMICVSRPTNPTGNVITD+EL+KLDALANQHGIPLVIDNAYGVPFPGIIFS+ARPLWNPN
Sbjct: 181 GMICVSRPTNPTGNVITDDELIKLDALANQHGIPLVIDNAYGVPFPGIIFSDARPLWNPN 240

Query: 241 IVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGIISLAPGGIGPAMMCEMIKRNDLLR 300
           IVLCMSLSKLGLPGSRCGIIIANEKIITAI+NMNGIISLAPGG+GPAMMCEMIKRNDLLR
Sbjct: 241 IVLCMSLSKLGLPGSRCGIIIANEKIITAISNMNGIISLAPGGMGPAMMCEMIKRNDLLR 300

Query: 301 LSETVIKPFYYQRVQETIAIIRRYLPENRCLIHKPEGAIFLWLWFKDLPITTKQLYQRLK 360
           LSETVIKPFYYQRVQETIAIIRRYLPE+RCLIHKPEGAIFLWLWFKDLPI+T+ LYQRLK
Sbjct: 301 LSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHKPEGAIFLWLWFKDLPISTELLYQRLK 360

Query: 361 ARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYVPEPEKIEAGVKILAEEIERAWAE 415
            RGVLMVPG  FFPGLDKPWPHTHQCMRMNYVP+PEKIEAGV+ILAEE+ERAW E
Sbjct: 361 KRGVLMVPGDYFFPGLDKPWPHTHQCMRMNYVPDPEKIEAGVRILAEEVERAWRE 415


Lambda     K      H
   0.322    0.141    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 758
Number of extensions: 18
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 417
Length of database: 419
Length adjustment: 32
Effective length of query: 385
Effective length of database: 387
Effective search space:   148995
Effective search space used:   148995
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis