Align 3-dehydroquinate synthase (EC 4.2.3.4) (characterized)
to candidate 199482 SO0287 3-dehydroquinate synthase (NCBI ptt file)
Query= BRENDA::P07639 (362 letters) >FitnessBrowser__MR1:199482 Length = 359 Score = 429 bits (1104), Expect = e-125 Identities = 216/358 (60%), Positives = 271/358 (75%), Gaps = 2/358 (0%) Query: 2 ERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNETLAPLYLDKVRGVLEQAG 61 ++I V LGERSYPI I L ++ + +++++VTNET+APLYL +V+ + G Sbjct: 3 QQIQVDLGERSYPIYIGQSLMSDSETLSRYLLKKRILIVTNETVAPLYLKQVQDTMASFG 62 Query: 62 VNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVALGGGVVGDLTGFAAASYQRG 121 + SVIL DGEQ+K L LD++FTALLQ+ +GRD+ LVALGGGV+GD+TGFAAA YQRG Sbjct: 63 -EISSVILSDGEQFKDLTHLDSIFTALLQRNYGRDSVLVALGGGVIGDMTGFAAACYQRG 121 Query: 122 VRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQPASVVVDLDCLKTLPPRELASG 181 + FIQ+PTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP V++D +CL+TLP RE A+G Sbjct: 122 IDFIQIPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQPQIVIIDTECLQTLPAREFAAG 181 Query: 182 LAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYCIRRCCELKAEVVAADERETGLRA 241 +AEVIKYGI+ D FF WLE N+ AL LD A+ Y I RCCE+KA+VV+ DE E G+RA Sbjct: 182 MAEVIKYGIMWDADFFQWLENNVQALKSLDTQALVYAISRCCEIKADVVSQDETEQGVRA 241 Query: 242 LLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAARTSERLGQFSSAETQRIITLLKRAG 301 LLNLGHTFGHAIEAEMGYGNWLHGEAVAAG V+AA+T++ LG + RI+ LL+ Sbjct: 242 LLNLGHTFGHAIEAEMGYGNWLHGEAVAAGTVLAAQTAKSLGLIDESIVCRIVQLLQAFD 301 Query: 302 LPVNGPREMSAQAYLPHMLRDKKVLAGEMRLILPLAIGKSEVRSGVSHELVLNAIADC 359 LPV P M ++++ HM RDKKVL G++RL+LP AIG++EV S V E L + C Sbjct: 302 LPVKAPESMDFESFIQHMRRDKKVLGGQIRLVLPTAIGRAEVFSQVP-ESTLEQVICC 358 Lambda K H 0.320 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 328 Number of extensions: 5 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 362 Length of database: 359 Length adjustment: 29 Effective length of query: 333 Effective length of database: 330 Effective search space: 109890 Effective search space used: 109890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate 199482 SO0287 (3-dehydroquinate synthase (NCBI ptt file))
to HMM TIGR01357 (aroB: 3-dehydroquinate synthase (EC 4.2.3.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01357.hmm # target sequence database: /tmp/gapView.18515.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01357 [M=344] Accession: TIGR01357 Description: aroB: 3-dehydroquinate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.5e-126 406.6 0.0 5.1e-126 406.5 0.0 1.0 1 lcl|FitnessBrowser__MR1:199482 SO0287 3-dehydroquinate synthase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__MR1:199482 SO0287 3-dehydroquinate synthase (NCBI ptt file) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 406.5 0.0 5.1e-126 5.1e-126 1 342 [. 14 355 .. 14 357 .. 0.96 Alignments for each domain: == domain 1 score: 406.5 bits; conditional E-value: 5.1e-126 TIGR01357 1 ykvkvgegllkklveelae..kasklvvitdeeveklvaekleealkslgvevlvlvvpdgeesKsletvaklldqlle 77 y++++g++l++ e+l++ +++++++t+e+v+ l+ ++++++ +s e+ ++++dge+ K+l+++ ++ ++ll+ lcl|FitnessBrowser__MR1:199482 14 YPIYIGQSLMSDS-ETLSRylLKKRILIVTNETVAPLYLKQVQDTMAS-FGEISSVILSDGEQFKDLTHLDSIFTALLQ 90 6789999998854.5555546679**********************99.779999************************ PP TIGR01357 78 eklerksvlvaiGGGvvgDlaGFvAatylRGirlvqvPTtllamvDssvGGKtginlplgkNliGafyqPkaVlidlkv 156 ++ r+svlva+GGGv+gD++GF+Aa y+RGi+++q+PTtll++vDssvGGKt++n+plgkN+iGafyqP+ V+id+++ lcl|FitnessBrowser__MR1:199482 91 RNYGRDSVLVALGGGVIGDMTGFAAACYQRGIDFIQIPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQPQIVIIDTEC 169 ******************************************************************************* PP TIGR01357 157 letlperelreGmaEviKhgliadaelfeelekneklllklaelealeelikrsievKaevVeeDekesglRalLNfGH 235 l+tlp re+++GmaEviK+g+++da++f++le+n ++l++l +++al +i+r++e+Ka+vV++De+e+g+RalLN+GH lcl|FitnessBrowser__MR1:199482 170 LQTLPAREFAAGMAEVIKYGIMWDADFFQWLENNVQALKSL-DTQALVYAISRCCEIKADVVSQDETEQGVRALLNLGH 247 ***********************************988765.66*********************************** PP TIGR01357 236 tlgHaiEallkyk.lsHGeaVaiGmvveaklseklgllkaellerlvallkklglptklkkklsveellkallkDKKne 313 t+gHaiEa+++y+ + HGeaVa G v++a+++++lgl++++++ r+v+ll+++ lp+k ++++++e++++++ +DKK lcl|FitnessBrowser__MR1:199482 248 TFGHAIEAEMGYGnWLHGEAVAAGTVLAAQTAKSLGLIDESIVCRIVQLLQAFDLPVKAPESMDFESFIQHMRRDKKVL 326 ******************************************************************************* PP TIGR01357 314 gskiklvlleeiGkaalasevteeellea 342 g++i+lvl+++iG+a++ s+v+e++l ++ lcl|FitnessBrowser__MR1:199482 327 GGQIRLVLPTAIGRAEVFSQVPESTLEQV 355 *********************99888775 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (344 nodes) Target sequences: 1 (359 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 9.25 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory