Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate 199942 SO0756 phospho-2-dehydro-3-deoxyheptonate aldolase, phe-sensitive (NCBI ptt file)
Query= BRENDA::C3TIE2 (350 letters) >FitnessBrowser__MR1:199942 Length = 352 Score = 530 bits (1365), Expect = e-155 Identities = 258/349 (73%), Positives = 298/349 (85%) Query: 1 MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIHKILKGNDDRLLVVIGP 60 M YQNDD+RI E+KELLPP+A+LE+FPA+ENAA TV +AR++IH IL DDRLLVVIGP Sbjct: 1 MYYQNDDVRINEVKELLPPIAILERFPASENAAATVFNARQSIHNILAREDDRLLVVIGP 60 Query: 61 CSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTVGWKGLINDPHMDNSFQIN 120 CSIHDP AA EY RL+ LRE +D+LEIVMRVYFEKPRTTVGWKGLINDP+MDNSF++N Sbjct: 61 CSIHDPKAALEYGQRLVVLRERYQDQLEIVMRVYFEKPRTTVGWKGLINDPYMDNSFKLN 120 Query: 121 DGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWGAIGARTTESQVHRELASGLS 180 DGLR ARKLL+D+NDSG+P AGEFLDMITPQY+AD+M WGAIGARTTESQVHRELASGLS Sbjct: 121 DGLRTARKLLVDLNDSGMPTAGEFLDMITPQYVADMMCWGAIGARTTESQVHRELASGLS 180 Query: 181 CPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHSAIVNTSGNGDCHIILRGGKEPN 240 CPVGFKNGTDGTIKVAIDAI AA APH FLSVTK+GHSAIV+T GN DCHIILRGG+EPN Sbjct: 181 CPVGFKNGTDGTIKVAIDAIGAANAPHHFLSVTKFGHSAIVSTKGNPDCHIILRGGREPN 240 Query: 241 YSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFKKQMDVCADVCQQIAGGEKAIIGVM 300 YSA HVA++ + L KA L +MIDFSHANSSKQ+++QM V +V +Q+A G +AI GVM Sbjct: 241 YSASHVAQISDQLKKAKLVDNIMIDFSHANSSKQYQRQMVVANEVAEQVAAGNQAIFGVM 300 Query: 301 VESHLVEGNQSLESGEPLAYGKSITDACIGWEDTDALLRQLANAVKARR 349 VESHLVEG Q L G+ L YG+S+TDACIGWED++ LL L +V RR Sbjct: 301 VESHLVEGRQDLIEGQALCYGQSVTDACIGWEDSERLLAVLNQSVVERR 349 Lambda K H 0.317 0.134 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 466 Number of extensions: 4 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 350 Length of database: 352 Length adjustment: 29 Effective length of query: 321 Effective length of database: 323 Effective search space: 103683 Effective search space used: 103683 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
Align candidate 199942 SO0756 (phospho-2-dehydro-3-deoxyheptonate aldolase, phe-sensitive (NCBI ptt file))
to HMM TIGR00034 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00034.hmm # target sequence database: /tmp/gapView.12113.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00034 [M=342] Accession: TIGR00034 Description: aroFGH: 3-deoxy-7-phosphoheptulonate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-174 566.1 0.0 1.2e-174 565.9 0.0 1.0 1 lcl|FitnessBrowser__MR1:199942 SO0756 phospho-2-dehydro-3-deoxy Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__MR1:199942 SO0756 phospho-2-dehydro-3-deoxyheptonate aldolase, phe-sensitive (NCBI ptt file) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 565.9 0.0 1.2e-174 1.2e-174 1 342 [] 6 349 .. 6 349 .. 0.99 Alignments for each domain: == domain 1 score: 565.9 bits; conditional E-value: 1.2e-174 TIGR00034 1 ddlrivkidelltPeelkakfpltekaaekvaksrkeiadilaGkddrllvviGPcsihdpeaaleyakrlkklaeklk 79 dd+ri +++ell+P ++ ++fp++e+aa++v ++r++i++ila +ddrllvviGPcsihdp+aaley +rl l+e+++ lcl|FitnessBrowser__MR1:199942 6 DDVRINEVKELLPPIAILERFPASENAAATVFNARQSIHNILAREDDRLLVVIGPCSIHDPKAALEYGQRLVVLRERYQ 84 799**************************************************************************** PP TIGR00034 80 ddleivmrvyfekPrttvGWkGlindPdlnesfdvnkGlriarkllldlvelglplatelldtispqyladllswgaiG 158 d+leivmrvyfekPrttvGWkGlindP++++sf++n+Glr arkll+dl++ g+p+a+e+ld+i+pqy+ad+++wgaiG lcl|FitnessBrowser__MR1:199942 85 DQLEIVMRVYFEKPRTTVGWKGLINDPYMDNSFKLNDGLRTARKLLVDLNDSGMPTAGEFLDMITPQYVADMMCWGAIG 163 ******************************************************************************* PP TIGR00034 159 arttesqvhrelasglslpvgfkngtdGslkvaidairaaaaehlflsvtkaGqvaivetkGnedghiilrGGkkpnyd 237 arttesqvhrelasgls+pvgfkngtdG++kvaidai aa+a+h+flsvtk G++aiv+tkGn+d+hiilrGG++pny+ lcl|FitnessBrowser__MR1:199942 164 ARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAIGAANAPHHFLSVTKFGHSAIVSTKGNPDCHIILRGGREPNYS 242 ******************************************************************************* PP TIGR00034 238 aedvaevkeelekaglkeelmidfshgnsnkdykrqlevaesvveqiaeGekaiiGvmiesnleeGnqsl..keelkyG 314 a++va++ ++l+ka+l +++midfsh+ns+k+y+rq+ va++v+eq+a G++ai+Gvm+es+l+eG+q+l +++l yG lcl|FitnessBrowser__MR1:199942 243 ASHVAQISDQLKKAKLVDNIMIDFSHANSSKQYQRQMVVANEVAEQVAAGNQAIFGVMVESHLVEGRQDLieGQALCYG 321 *********************************************************************988899**** PP TIGR00034 315 ksvtdacigwedteallrklaeavkerr 342 +svtdacigwed+e+ll+ l+++v err lcl|FitnessBrowser__MR1:199942 322 QSVTDACIGWEDSERLLAVLNQSVVERR 349 ***********************99886 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (342 nodes) Target sequences: 1 (352 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 9.41 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory