Align glutamate--ammonia ligase; EC 6.3.1.2 (characterized)
to candidate 203487 SO4410 glutamine synthetase, type I (NCBI ptt file)
Query= CharProtDB::CH_024014 (469 letters) >FitnessBrowser__MR1:203487 Length = 469 Score = 752 bits (1941), Expect = 0.0 Identities = 364/469 (77%), Positives = 408/469 (86%) Query: 1 MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFFEEGKMFDGSSIGGWKG 60 MS E VL L E EVKFVDLRFTDTKGKEQHV+IP+HQV+A+FFE+GKMFDGSSI GWKG Sbjct: 1 MSVESVLKQLEELEVKFVDLRFTDTKGKEQHVSIPSHQVDADFFEDGKMFDGSSIAGWKG 60 Query: 61 INESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYDRDPRSIAKRAEDYLRSTG 120 INESDMVLMPD +T V+DPF ++T +IRCD+LEPGT+ GYDRDPRSIAK+AE+YLRSTG Sbjct: 61 INESDMVLMPDPTTFVLDPFTEETTALIRCDVLEPGTMTGYDRDPRSIAKKAEEYLRSTG 120 Query: 121 IADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWNSSTQYEGGNKGHRPAVKGGY 180 IADTVL GPEPEFFLFDD+RFG+ +SG V ID E AWNS T YEGGN GHRP VKGGY Sbjct: 121 IADTVLVGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWNSGTSYEGGNTGHRPFVKGGY 180 Query: 181 FPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAGQNEVATRFNTMTKKADEIQIYK 240 FPV PVDS+QD+RS MCLV+E+MG VVEAHHHEVATAGQNE+ATRFNT+TKKADEIQI K Sbjct: 181 FPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK 240 Query: 241 YVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLSKNGVNLFAGDKYAGLSEQALYYIG 300 YVVHN+AH +GKTATFMPKP+ GDNGSGMH H SLSK+GVNLFAGDKYAGLSE ALYYIG Sbjct: 241 YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLSKDGVNLFAGDKYAGLSETALYYIG 300 Query: 301 GVIKHAKAINALANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVSSPKARRIEVR 360 G+IKHA+A+NA NP+TNSYKRLVP +EAPVMLAYSARNRSASIRIPVV SPK RRIE R Sbjct: 301 GIIKHARALNAFTNPSTNSYKRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETR 360 Query: 361 FPDPAANPYLCFAALLMAGLDGIKNKIHPGEAMDKNLYDLPPEEAKEIPQVAGSLEEALN 420 FPDP ANPYL FAALLMAG+DGI+NKIHPGEAMDK+LYDLP EEA EIPQVA SLE AL Sbjct: 361 FPDPHANPYLGFAALLMAGIDGIQNKIHPGEAMDKDLYDLPAEEAAEIPQVATSLENALE 420 Query: 421 ELDLDREFLKAGGVFTDEAIDAYIALRREEDDRVRMTPHPVEFELYYSV 469 L D EFL GGVF+++ I +YIAL+ E +RV T HP+EFE+YYS+ Sbjct: 421 NLQADHEFLTRGGVFSEDFIQSYIALKSAEAERVARTTHPLEFEMYYSL 469 Lambda K H 0.318 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 771 Number of extensions: 17 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 469 Length of database: 469 Length adjustment: 33 Effective length of query: 436 Effective length of database: 436 Effective search space: 190096 Effective search space used: 190096 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate 203487 SO4410 (glutamine synthetase, type I (NCBI ptt file))
to HMM TIGR00653 (glnA: glutamine synthetase, type I (EC 6.3.1.2))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00653.hmm # target sequence database: /tmp/gapView.19357.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00653 [M=462] Accession: TIGR00653 Description: GlnA: glutamine synthetase, type I Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.6e-210 684.0 0.0 5.2e-210 683.9 0.0 1.0 1 lcl|FitnessBrowser__MR1:203487 SO4410 glutamine synthetase, typ Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__MR1:203487 SO4410 glutamine synthetase, type I (NCBI ptt file) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 683.9 0.0 5.2e-210 5.2e-210 2 462 .] 5 468 .. 4 468 .. 0.99 Alignments for each domain: == domain 1 score: 683.9 bits; conditional E-value: 5.2e-210 TIGR00653 2 evlkllkeenvkfvdlrfvDikGklkkveipvseleeealeegiaFDgssveGfksieesDlllkpdpetlvivPfrae 80 +vlk l+e +vkfvdlrf+D+kGk+++v+ip ++++++++e+g++FDgss+ G+k+i+esD++l+pdp t+v +Pf++e lcl|FitnessBrowser__MR1:203487 5 SVLKQLEELEVKFVDLRFTDTKGKEQHVSIPSHQVDADFFEDGKMFDGSSIAGWKGINESDMVLMPDPTTFVLDPFTEE 83 6889999************************************************************************ PP TIGR00653 81 kvlrvicdvyepvtkepyerdpRsiakraeeelk.tklgdevyfGpEaEFflfdkvefkeasnssflevdseegewn.. 156 +++ ++cdv ep t++ y+rdpRsiak+aee+l+ t+++d+v +GpE+EFflfd+v+f++ ++ f+++d++e++wn lcl|FitnessBrowser__MR1:203487 84 TTALIRCDVLEPGTMTGYDRDPRSIAKKAEEYLRsTGIADTVLVGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWNsg 162 *****************************************************************************99 PP TIGR00653 157 reveegnkgykikkkggYfdvepvDkakdirrelvlaleelglevevsHHEvata.qaEidikfdklvkaaDeivlyKy 234 +++e+gn+g+++ kggYf+v+pvD+ +d+r+ ++l+lee+g ve++HHEvata q+Ei+++f++l+k+aDei+++Ky lcl|FitnessBrowser__MR1:203487 163 TSYEGGNTGHRPFVKGGYFPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAgQNEIATRFNTLTKKADEIQILKY 241 99***************************************************************************** PP TIGR00653 235 vvknvakkhGktatFmpKplfgdngsGmHvhlslwkdgenlfageegyagLsetalyyigGilkHakalaAltnptvns 313 vv+n+a+ +GktatFmpKp++gdngsGmHvh+sl+kdg nlfag++ yagLsetalyyigGi+kHa+al+A+tnp++ns lcl|FitnessBrowser__MR1:203487 242 VVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLSKDGVNLFAGDK-YAGLSETALYYIGGIIKHARALNAFTNPSTNS 319 **********************************************.******************************** PP TIGR00653 314 YkRLvpGyEAPvylaysaknRsaliRiPasanpkakRiEvRspDpsanpYLafaallmAgldGiknkidpgepldknly 392 YkRLvp +EAPv+laysa+nRsa+iRiP++ +pk++RiE R+pDp anpYL faallmAg+dGi+nki+pge++dk+ly lcl|FitnessBrowser__MR1:203487 320 YKRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETRFPDPHANPYLGFAALLMAGIDGIQNKIHPGEAMDKDLY 398 ******************************************************************************* PP TIGR00653 393 elseeelkelGieqlpesLeealdelesdk..evlkevlgeelieafielkrkEveelrlkvhpvElekyld 462 +l+ ee++e i+q+++sLe+al++l++d+ ++ ++v++e++i+++i+lk +E e++ + hp+E+e+y++ lcl|FitnessBrowser__MR1:203487 399 DLPAEEAAE--IPQVATSLENALENLQADHefLTRGGVFSEDFIQSYIALKSAEAERVARTTHPLEFEMYYS 468 *********..*******************888999**********************************96 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (462 nodes) Target sequences: 1 (469 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 8.48 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory