Alignments for a candidate for aceA in Shewanella oneidensis MR-1

GapMind for Amino acid biosynthesis

Alignments for a candidate for aceA in Shewanella oneidensis MR-1

Align Bifunctional glyoxylate cycle protein; Gex-3-interacting protein 7; EC 4.1.3.1; EC 2.3.3.9 (characterized)
to candidate 200654 SO1483 malate synthase A (NCBI ptt file)

Query= SwissProt::Q10663
         (968 letters)



>FitnessBrowser__MR1:200654
          Length = 549

 Score =  483 bits (1243), Expect = e-140
 Identities = 260/529 (49%), Positives = 326/529 (61%), Gaps = 9/529 (1%)

Query: 443 LSLTAQNVAGDEKILTPDALRFLHDLNTEFNPRRLRLLSKRNQVQADINNSLWFPDFNKE 502
           L+L    + G E I T  A+  L  L  EF      LL+KR   QA I+     PDF  E
Sbjct: 24  LALVGNTIPGQEVIFTEGAMALLESLCREFGAEVPTLLAKRKDRQARIDKGA-LPDFLPE 82

Query: 503 TEVLRSDQGWKGAEIPRDLQDRRVEITGPTDRKMVINAMNSGANVFMADFEDSNSPTWRN 562
           T  +R D  WK   IP DL DRRVEITGP +RKMVINA+N+ A VFMADFEDS +P+W+ 
Sbjct: 83  TRAIR-DGAWKIRGIPNDLLDRRVEITGPVERKMVINALNANAKVFMADFEDSLAPSWQK 141

Query: 563 QLEGQINLYDAVRNNISYTHPTTKKEYTLNEKHAVLKVRPRGWHLPEKHVLIHNQPTSGS 622
            +EGQINL DAVR  I YT P T K Y L    AVL  R RG HL EKHV  + Q   G 
Sbjct: 142 VVEGQINLRDAVRGEIEYTAPETGKHYKLGPNPAVLICRVRGLHLKEKHVEFNQQSIPGG 201

Query: 623 LFDFGLFVFHNAKALIAQGSGPYFYLPKLQSAEEAQLWADVFKYTEDKLGLARGTIKCTV 682
           LFDF ++ +HN + L+A+GSGPYFY+PKL+S  EA+ WA VF + E++  L  GTIKCT 
Sbjct: 202 LFDFAMYFYHNYRQLLAKGSGPYFYIPKLESHIEARWWAKVFAFVEERFCLQAGTIKCTC 261

Query: 683 LIEHLLASFQLHEIIHALKDNIVGLNCGRWDYIFSYIKTFQNHRKFLLPDRFQIGMTAPF 742
           LIE L A F++ EI++ L+ NIV LNCGRWDYIFSYIKT + H   +LPDR  + M  PF
Sbjct: 262 LIETLPAVFEMDEILYELRSNIVALNCGRWDYIFSYIKTLKRHGDRVLPDRQAVTMDTPF 321

Query: 743 MRNYSLEVIKACHLRGIHAMGGMAAQIPIKHDQVANDKAFALVRADKEREATDGHDGTWV 802
           +  YS  +IK CH RG  AMGGMAA IP K D   N+     VR DKE EA +GHDGTWV
Sbjct: 322 LSAYSRLLIKTCHKRGALAMGGMAAFIPAK-DPAQNEAVLQRVRKDKELEARNGHDGTWV 380

Query: 803 AHPGLVPLAKRVFDQMMPKPNQISKNLTR---ANCTKEDLTVIPEGTRTEAGFRHNISVT 859
           AHPGL   A  +F++ + + +Q   ++TR   A     +L    +G RTE G R NI + 
Sbjct: 381 AHPGLADTAMGIFNEYIGQDHQNQLHITRDVDAPILAAELLKTCDGERTEQGMRLNIRIA 440

Query: 860 LGYLDSWLRGTGCVPLYNLMEDAATAEISRAQLWQWLHHDAKLEDGRTIDAGLVKQTIAA 919
           L YL++W+ G GCVP+Y LMEDAATAEISRA +WQW+ H   L +G+ +   L K  +  
Sbjct: 441 LQYLEAWISGNGCVPIYGLMEDAATAEISRASIWQWIQHGKSLSNGKLVTKQLFKDMLVE 500

Query: 920 ETERRLIRAGS---VVNRIPEAADLLEKFVTEEKMSDFLTTDAYDRLVS 965
           E        GS      +  +AA LLE   T +++ DFLT   Y+ L +
Sbjct: 501 ELANVKKEVGSDRFTHGKFTQAAVLLEDITTSDELVDFLTLPGYEMLTA 549


Lambda     K      H
   0.319    0.134    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1170
Number of extensions: 41
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 968
Length of database: 549
Length adjustment: 40
Effective length of query: 928
Effective length of database: 509
Effective search space:   472352
Effective search space used:   472352
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis